Publications
2023
Wilder, C.S., Chiou, J., Battenhouse, A., Saha, A., Chen, Z., Kim, E., Gadallah, M.I., Tiziani, S., Georgiou, G., Stone, E., and J. DiGiovanni, “Enzymatic Depletion of L-Met Using an Engineered Human Enzyme as a Novel Therapeutic Strategy for Melanoma,” Mol Carcinogenesis (2023) PMID: 37378415 doi: 10.1002/mc.23597.
Saha, A., Zhao, S., Kindall, A., Wilder, C., Friedman, C.A., Clark, R., Georgiou, G., Stone, E., Kidane, D., and J. DiGiovanni, “Cysteine Depletion Sensitizes Prostate Cancer Cells to Agents that Enhance DNA Damage and to Immune Checkpoint Inhibition,” J. Exp. Clin. Cancer Res. 42:119 (2023) PMID: 37170264 doi: 10.1186/s13046-023-02677-2.
2022
Blazeck, J., Karamitros, S.K, Ford, K., Somody, C., Qerqez, A., Murray, K., Burkholder, N.T., Marshall, N., Sivakumar, A., Lu, W-C., Tan, B., Lamb, C., Tanno, Y., Siddiqui, M.N., Ashoura, N., Coma, S., Zhang, X., McGovern, K., Kumada, Y. Zhang, Y.J., Manfredi, M., Johnson, K.A., S., D’Arcy, Stone, E. and G. Georgiou, “Bypassing Evolutionary Dead-ends and Switching the Rate-Limiting Step of a Human Immunotherapeutic Enzyme,” Nature Catalysis 5:952-967 (2022) PMID: 36465553 doi: 10.1038/s41929-022-00856-6.
Kuraoka, M., Curtis, N.C., Watanabe, A., Tanno, H., Shin, S., Ye, K., Macdonald, E., Lavidor, O., Kong, S., Von Holle, T., Windsor, I., Ippolito,G.C., Georgiou, G., Walter, E.B., Kelsoe, G., Harrison, S.C., Moody, M.A., Bajic, G. and J. Lee, “Infant Antibody Repertoires during the First Two Years of Influenza Vaccination,” mBio 13:e0254622 (2022) PMID: 36314798 doi: 10.1128/mbio.02546-22.
Zhang, L., Hobeika, C.S., Khabibullin, D., Yu, D., Filippakis, H., Alchoueiry, M., Tang, Y., Lam, H.C., Tsvetkov, P., Georgiou, G., Lamb, C., Stone, E., Puigserver, P., Priolo, C. and E.P. Henske, “Hypersensitivity to ferroptosis in chromophobe RCC is mediated by a glutathione metabolic dependency and cystine import via solute carrier family 7 member 11” Proc Natl Acad Sci U S A 119:e2122840119 (2022) PMID: 35867762 doi: 10.1073/pnas.2122840119
Karamitros, C.S., Murray, K., Winemiller, B., Lamb, C., Stone, E.M., D’Arcy, S., Johnson, K.A., and G. Georgiou, “Leveraging Intrinsic Flexibility to Engineer Enhanced Enzyme Catalytic Activity,” Proc. Natl. Acad. Sci. USA 119:e2118979119 (2022) PMID: 35658075 doi: 10.1073/pnas.2118979119
Delidakis, G., Kim, J.E., George, K. and G. Georgiou “Improving Antibody Therapeutics byManipulating the Fc Domain: Immunological and Structural Considerations” Ann. Rev. Biomed. Engin. 6:249-74 (2022) PMID: 35363537 doi: 10.1146/annurev-bioeng-082721-024500
Pataskar, A., Champagne, J., Nagel, R., Kenski, J., Laos, M., Michaux, J., Pak, H.S., Bleijerveld, O.B., Mordente, K., Navarro, J.M., Blommaert, N., Nielsen, N.M., Lovecchio, D., Stone, E., Georgiou G., de Gooijer, M.C., van Tellingen, O., Altelaar, M., Joosten, P.R., Perrakis, A., Olweus, J., Bassani-Sternberg, M., Peeper, D.M. and R. Agami, “Tryptophan Depletion Results in Tryptophan-to-Phenylalanine Substituants.” Nature 603:721-727 (2022) PMID: 35264796 doi: 10.1038/s41586-022-04499-2.
2021
Charab W, Rosenberger MG, Shivram H, Mirazee JM, Donkor M, Shekhar SR, Gjuka D, Khoo KH, Kim JE, Iyer VR, Georgiou G., “IgG Immune Complexes Inhibit Naïve T Cell Proliferation and Suppress Effector Function in Cytotoxic T Cells,” Front Immunol.12:713704 (2021) PMID: 34447380 doi: 10.3389/fimmu.2021.713704.
Jung, J., Mundle, S.T., Ustyugova, I.V., Horton, A.P., Boutz, D.R., Pougatcheva, S., Ponraj, P., McDaniel, J.R., King, G.R., Park, D., Person, M.D., Ye, C., Tan, B., Tanno, Y., DiNapoli, J., Delagrave, S., Ross, T.M., Ippolito, G.C., Kleanthous, H., Lee, J. and G. Georgiou, “High Prevalence of Serum Monoclonal Antibodies to Non-Hemagglutinin Antigens Including Sulfated Glycans in the Elderly after Seasonal Influenza Vaccination,” J. Clinical Investigations 131:e148763 (2021) PMID: 34196304 doi: 10.1172/JCI148763.
McCarthy, K.R., Lee, J. Watanabe, A., Kuraoka, M., Robinson-McCarthy, L-R., Georgiou, G., Kelsoe, G., and Stephen C. Harrison, “A Prevalent Focused Human Antibody Response to the Influenza Hemagglutinin Head Interface,” mBio 2:e0114421 (2021) PMID: 34060327 doi: 10.1128/mBio.01144-21.
Voss WN, Hou YJ, Johnson NV, Delidakis G, Kim JE, Javanmardi K, Horton AP, Bartzoka F, Paresi CJ, Tanno Y, Chou C-W, Abbasi SA, Pickens W, George K, Boutz DR, Towers DM, McDaniel JR, Billick D, Goike J, Rowe L, Batra D, Pohl J, Lee J, Gangappa S, Sambhara S, Gadush M, Wang N, Person MD, Iverson BL, Gollihar JD, Dye J, Herbert A, Finkelstein IJ, Baric RS, McLellan JS, Georgiou G, Lavinder JJ*, Ippolito GC*. Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes. Science 372:1108-1112 (2021) PMID: 33947773 doi: 10.1126/science.abg5268.
Saha, A., Zhao, S., Chen, Z., Georgiou, G., Stone, E., Kidane, D. and J. DiGiovanni, “Combinatorial Approaches to Enhance DNA Damage following Enzyme-Mediated Depletion of L-Cys for Treatment of Pancreatic Cancer” Molecular Therapeutics,” 29:775-787. (2021) PMID: 33091613 doi: 10.1016/j.ymthe.2020.10.016
Denard, C.A., Paresi, C., Yaghi, R., McGinnis, N., Bennett, Z., Yi, L., Georgiou, G. and B.L. Iverson,
“YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants”ACS Synth Biol. 15:63-71 (2021) PMID: 33401904 doi: 10.1021/acssynbio.0c00452.
Karamitros, C.S., Murray, K., Sugiyama, Y., Kumada, Y., Johnson, K.A., Georgiou, G., D’Arcy, S., E.M. “Conformational Dynamics Contribute to Substrate Selectivity and Catalysis in Human Kynureninase” ACS Chem Biol. 18:3159-3166. (2021) PMID: 33275413 doi: 10.1021/acschembio.0c00676.
2020
Mehaffey M.R., Lee J., Jung J., Lanzillotti M.B., Escobar E.E., Morgenstern K.R., Georgiou G., Brodbelt J.S., “Mapping a Conformational Epitope of Hemagglutinin A Using Native Mass Spectrometry and Ultraviolet Photodissociation,” Anal Chem 92(17):11869-11878 (2020) PMID: 32867493 DOI: 10.1021/acs.analchem.0c02237.
Verardi, R., Lindesmith, L.C., Tsybovsky, Y., Gorman, J., Chuang, Y.-G., Edwards, K., Jensen, P.B., Mallory, M.L., Ou, L., Schön, A., Tully, E.S. Georgiou, G. Baric, R.S. and P.D. Kwong “Disulfide-Stabilization of Human Norovirus GI.1 Virus-like Particles Focuses Immune Response Toward Neutralizing Epitopes” NPJ Vaccines (2020) in press.
Lyu, A., Triplett, T.A., Nam, S.H., Hu, Z., Arasappan, D., Godfrey, H.D., Ames, R.Y., Sarang, A., Selden, H.J., Lee, C-H., Georgiou, G., Horton, T.M., Ehrlich, L.I.R. “Tumor-Associated Myeloid Cells Provide Critical Support for T-ALL”. Blood DOI: 10.1182/blood.2020007145 PMID: 32845007
Soni, C., Perez, O.A., Voss, W.N., Pucella, J.N., Serpas, L., Mehl, J., Ching, K.L., Goike, J., Georgiou, G.,
Ippolito, G.C., Sisirak, V. and Reizis, B. “Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA” Immunity 52:1022-1038 (2020) PMID: 32454024 DOI:10.1016/j.immuni.2020.04.015
Lu, W-C., Saha, A., Yan, W., Garrison, K., Lamb, C., Pandey, R., Irani, S., Lodi, A., Lu, X., Tiziani, S., Zhang., Y.J., Georgiou, G., DiGiovanni, J. and E. Stone, “Enzyme-Mediated Depletion of Serum L-Met Abrogates Prostate Cancer Growth via Multiple Mechanisms without Evidence of Systemic Toxicity, “Proc. Natl. Acad. Sci. USA 117:532-540 (2020) PMID: 32434918, DOI: 10.1073/pnas.1917362117
Badgley, M.A., Kremer, D., Maurer, H.C., DelGiorno, K.E., Lee, H-J., Purohit, H., Sagalovskiy, I., Ma, A., Kapillian, J., Firl, E.M., Decker, A.R., Sastra, S.A, Palermo, C.F., Andrade, L.R., Sajjakulnukit, P., Zhang, L., Tolstyka, Z.P., Hirschhorn, T., Lamb, C., Liu, T., Gu, W., Seeley, E.S., Stone, E., Georgiou, G., Manor, U., Iuga, A., Wahl, G. F., Stockwell, B.R., Lyssiotis, C.A. and K. P. Olive, “Cysteine depletion induces pancreatic tumor ferroptosis in mice, “ Science 368:85-89 (2020) PMID: 32241947, DOI: 10.1126/science.aaw9872
Tanno, H., McDaniel, J.R., Stevens, C.A., Voss, W.N., Li. J., Durrett, R., Lee, J., Gollihar, J., Tanno, Y., Delidakis, G., Pothukuchy, A. Ellefson, J.W., Goronzy, J.J., Maynard, J.A., Ellington, A.D., Ippolito, G.C. and G. Georgiou, “A Facile Technology for the High Throughput Sequencing of the Paired VH:VL and TCR:TCR Repertoires ,” Science Advances 6:eaay9093 (2020) PMID: 32426460, DOI: 10.1126/sciadv.aay9093.
Tanno, H., Gould, T.M., McDaniel, J.R., Cao,W., Tanno, Y., Durrett, R.E., Park, D., Cate, S.J., Hildebrand, W.H., Dekker, C.L., Tian, L., Weyand, C.M., Georgiou, G. and J.J., Goronzy, “Determinants Governing T cell Receptor α/β-Chain Pairing in Repertoire Formation of Identical Twins,” Proc. Natl. Acad. Sci. USA 117:532-540 (2020) PMID: 31879353, DOI: 10.1073/pnas.1915008117
Lee, J., Der, B.S., Karamitros, C.S., Li, W., Marshall, N.M., Lungu, O.A., Miklos , A.E., Xu , J., Kang, T-H., Lee, C-H., Tan, B., Hughes , R.A., Jung, S-T., Ippolito , G.C.. Gray, J.J., Zhang, Y., Kuhlman, B., Georgiou G. and A.D. Ellington, “Computer-Based Engineering of Thermostabilized Antibody Fragments,” AICHE J., 66: e16864 (2020) DOI: 10.1002/aic.16864
Sterrett S, Peng BJ, Burton RL, LaFon DC, Westfall AO, Singh S, Pride M, Anderson AS, Ippolito GC, Schroeder HW Jr, Nahm MH, Krishna Prasad A, Goepfert P, Bansal A. Peripheral CD4 T follicular cells induced by a conjugated pneumococcal vaccine correlate with enhanced opsonophagocytic antibody responses in younger individuals. Vaccine. 2020 Feb 11;38(7):1778-1786. doi: 10.1016/j.vaccine.2019.12.023. Epub 2020 Jan 3. PMID: 31911030.
2019
Dekker JD, Baracho GV, Zhu Z, Ippolito GC, Schmitz RJ, Rickert RC, Tucker HO. Loss of the FOXP1 Transcription Factor Leads to Deregulation of B Lymphocyte Development and Function at Multiple Stages. Immunohorizons. 2019 Oct 7;3(10):447-462. PMID: 31591252. DOI: 10.4049/immunohorizons.1800079.
Taft, J.M., Georgeon, S., Allen, C., Reckel, S., DeSautelle, J., Hantschel, O., Georgiou, G., Iverson, B.L., “Rapid Screen for Tyrosine Kinase Inhibitor Resistance Mutations and Substrate Specificity,” ACS Chem Biol. 14(9):1888-1895 (2019) PMID: 31339688, DOI: 10.1021/acschembio.9b00283.
Kshattry, S., Saha, A., Gries, P., Tiziani, S., Stone, EM., Georgiou, G., DiGiovanni, J., “Enzyme-mediated depletion of l-cyst(e)ine synergizes with thioredoxin reductase inhibition for suppression of pancreatic tumor growth,” NPJ Precis Oncol. (2019) eCollection 2019, PMID: 31231686. DOI: 10.1038/s41698-019-0088-z.
Lindesmith, L.C., McDaniel, J.R., Changela, A., Varardi, R., Kerr S.A., Constantini, V., Brewer-Jensen, P.D., Mallory, M.L., Voss, W.N., Boutz, D.R., Blazeck, J. J., Ippolito, G.C., Vinje, J., Kwong, P.D., Georgiou, G. and Baric, R. “Boosted Broad Binding/Blockade Antibodies Dominate the Serological Repertoire to Norovirus Vaccine” Immunity 50: 1530-1541(2019) PMID: 31216462 DOI: 10.1016/j.immuni.2019.05.007
Wang, W., Green, M., Choi, J.E., Gijón, M., Kennedy, P.D., Johnson, J.K., Liao, P., Lang, X., Kryczek, I., Sell, A., Xia, H., Zhou, J., Li, G., Li, J., Li, W., Wei, S., Vatan, L., Zhang, H., Szeliga, W., Gu, W., Liu, R., Lawrence, T.S., Lamb, C., Tanno, Y., Cieslik, M., Stone, E., Georgiou. G., Chan, T.A., Chinnaiyan, A. and W. Zou, “CD8+ T Cells Regulate Tumour Ferroptosis During Cancer Immunotherapy” Nature (2019) PMID: 31043744 DOI: 10.1038/s41586-019-1170-y
Kang, T.H., Lee, C.H., Delidakis, G., Jung, J., Richard-Le Goff, O., Lee, J., Kim, J.E., Charab, W., Bruhns, P. and G. Georgiou, “An Engineered Human Fc variant With Exquisite Selectivity for FcγRIIIaV158 Reveals That Ligation of FcγRIIIa Mediates Potent Antibody Dependent Cellular Phagocytosis With GM-CSF-Differentiated Macrophages,” Front Immunology 10:562 (2019) PMID: 30984171 DOI: 10.3389/fimmu.2019.00562
Krebs, S.J., Kwon, Y.D., Schramm, C.A., Law, W.H., Donofrio, G., Zhou, K.H., Gift, S., Dussupt, V., Georgiev, I.S., Schätzle, S., McDaniel, J.R., Lai, Y.T., Sastry, M., Zhang, B., Jarosinski, M.C., Ransier, A., Chenine, A.L., Asokan, M., Bailer, R.T., Bose, M., Cagigi, A., Cale, E.M., Chuang, G.Y., Darko, S., Driscoll, J.I., Druz, A., Gorman, J., Laboune, F., Louder, M.K., McKee, K., Mendez, L., Moody, M.A., O’Sullivan, A.M., Owen, C., Peng, D., Rawi, R., Sanders-Buell, E., Shen, C.H., Shiakolas, A.R., Stephens, T., Tsybovsky, Y., Tucker, C., Verardi, R., Wang, K., Zhou, J., Zhou, T., Georgiou, G., Alam, S.M., Haynes, B.F., Rolland, M., Matyas, G.R., Polonis, V.R., McDermott, A.B., Douek, D.C., Shapiro, L., Tovanabutra, S., Michael, N.L., Mascola, J.R., Robb, M.L., Kwong, P.D. and N.A. Doria-Rose, “Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual,” Immunity 50:677-691 (2019) PMID: 30876875 DOI: 10.1016/j.immuni.2019.02.008
Lee, J., Paparoditis, P., Horton, A.P., Frühwirth, A., McDaniel, J.R., Jung, J., Boutz, D.R., Hussein, D.A., Tanno, Y., Pappas, L., Ippolito, G.C., Corti, D., Lanzavecchia, A. and G. Georgiou, “Persistent Antibody Clonotypes Dominate the Serum Response to Influenza over Multiple Years and Repeated Vaccinations,” Cell Host Microbe 25:367-376 (2019) PMID: 30795981 DOI: 10.1016/j.chom.2019.01.010
Manso, T.C., Groenner-Penna, M., Minozzo, J.C., Antunes, B.C., Ippolito, G.C., Molina, F., and Felicori, L.F., “Next-generation sequencing reveals new insights about gene usage and CDR-H3 composition in the horse antibody repertoire,” Mol. Immunology 105:251-259 (2019) PMID: 30562645
2018
Johnson, E.L., Doria-Rose, N.A., Gorman, J., Bhiman, J.N., Schramm, C.A., Vu, A.Q., Law, W.H., Zhang, B., Bekker, V., Karim, S.S.A., Ippolito, G.C., Morris, L., Moore, P.L. Kwong, P.D., Mascola, J.R. and G. Georgiou, “Sequencing HIV-neutralizing antibody exons and introns reveals detailed aspects of lineage maturation” Nature Communications 9(1):4136 (2018) PMID: 30297708 DOI:10.1038/s41467-018-06424-6
Saphire, E.O., Schendel, S.L., Fusco, M.L., Gangavarapu, K., Gunn, B.M., Wec, A.Z., Halfmann, P.J., Brannan, J.M., Herbert, A.S., Qiu, X., Wagh, K., He, S., Giorgi, E.E., Theiler J, Pommert KBJ, Krause TB, Turner HL, Murin CD, Pallesen J, Davidson E, Ahmed R, Aman MJ, Bukreyev A, Burton DR, Crowe JE Jr, Davis CW, Georgiou G, Krammer F, Kyratsous CA, Lai JR, Nykiforuk C, Pauly MH, Rijal P, Takada A, Townsend AR, Volchkov V, Walker LM, Wang CI, Zeitlin L, Doranz BJ, Ward AB, Korber B, Kobinger GP, Andersen KG, Kawaoka Y, Alter G, Chandran K, Dye JM; Viral Hemorrhagic Fever Immunotherapeutic Consortium, “Systematic Analysis of Monoclonal Antibodies against Ebola Virus GP Defines Features that Contribute to Protection,” Cell 2018 Aug 9;174(4):938-952.e13. doi: 10.1016/j.cell.2018.07.033. PMID: 30096313
Triplett, T.A., Garrison, K.C., Marshall, N., Donkor, M., Blazeck, J., Lamb, C., Qerqez A., Dekker, J.D., Tanno, Y. Lu, W-C., Karamitros, S.K., Ford, K., Tan, B., Zhang, M., McGovern, K., Coma, S., Kumada, Y., Yamany, M.S., Sentandreu, S., Fromm, G., Tiziani, S., Schreiber, T.H., Manfredi, M., Ehrlich, L.I.R., Stone E., and G. Georgiou, “Reversal of IDO-Mediated Cancer Immune Suppression by Systemic Kynurenine Depletion with a Therapeutic Enzyme,” Nature Biotechnology doi: 10.1038/nbt.4180
Lee, J. and G. Georgiou, “High Affinity IgA Against Microbial Antigens” Nature Immunology 2018 19:514-515 (2018) PMID: 29777208
Liu, Y., McDaniel, J.R., Khan, S., Campisi, P., Propst, E.J., Holler, T., Grunebaum, E., Georgiou, G., Ippolito, G.C. and Ehrhardt, G.R.A., “Antibodies Encoded by FCRL4-Bearing Memory B Cells Preferentially Recognize Commensal Microbial Antigens,” J Immunology 200:3962-3969 (2018) PMID: 29703863
Chen, Y.Q., Wohlbold, T.J., Zheng, N.Y., Huang, M., Huang, Y., Neu, K.E., Lee, J., Wan, H., Rojas. K.T., Kirkpatrick., E., Henry, C., Palm, A.E., Stamper, C.T., Lan, L.Y., Topham, D.J., Treanor, J., Wrammert, J., Ahmed, R., Eichelberger, M.C., Georgiou, G, Krammer, F. and Wilson, P.C., “Influenza Infection in Humans Induces Broadly Cross-Reactive and Protective Neuraminidase-Reactive Antibodies,” Cell 173:417-429 (2018). PMID: 29625056
- Commentaries and News Stories: Covered by 9 scientific and mainstream media publications
McDaniel, J.R., Pero, S.C., Voss, W.N., Shukla, G.S., Sun, Y., Schaetzle, S., Lee, C.H., Horton, A.P., Harlow, S., Gollihar, J., Ellefson, J.W., Krag, C.C., Tanno, Y., Sidiropoulos, N., Georgiou, G., Ippolito, G.C. and Krag, D.N., “Identification of Tumor-Reactive B Cells and Systemic IgG in Breast Cancer Based on Clonal Frequency in the Sentinel Lymph Node,” Cancer Immunology Immunother 67:729-738 (2018) PMID: 29427082
Wang, B., DeKosky, B.J., Timm, M.R., Lee, J., Normandin, E., Misasi, J., Kong, R., McDaniel, J.R., Delidakis, G., Leigh, K.E., Niezold, T., Choi, C.W., Viox, E.G., Fahad, A., Cagigi, A., Ploquin, A., Leung, K., Yang, E.S., Kong, W.P., Voss, W.N., Schmidt, A.G., Moody, M.A., Ambrozak, D.R., Henry, A.R., Laboune, F., Ledgerwood, J.E., Graham, B.S., Connors, M., Douek, D.C., Sullivan, N.J., Ellington, A.D., Mascola, J.R. and G. Georgiou, “Functional Interrogation and Mining of Natively Paired Human VH:VL Antibody Repertoires,” Nature Biotechnology 36:152-155 (2018) PMID: 29309060
2017
McDaniel, J.R., Ippolito G.C. and G. Georgiou, “Mapping the Secrets of the Antibody Pool,” Nature Biotechnology 35:921-922. (2017) PMID: 28892077.
Jang, S-H., Gregersen, P.K., Georgiou, G., Diamond, B., “Increased Cathepsin S in Dendritic Cells Alters TFH Repertoire and Contributes to Lupus,”, Nature Immunology 18:1016-1024 (2017) PMID: 28692065
Cotham, V.C., Horton, A.P., Lee, J., Georgiou, G. and Brodbelt, J.S., “Middle-down 193-nm Ultraviolet Photodissociation for Unambiguous Antibody Identification and its Implications for Immunoproteomic Analysis,” Anal. Chem. 89:6498-6504 (2017) PMID: 28517930
Lee, C.-H., Romain, G., Yan, W., Watanabe, W., Charab, W., Todorova, B., Lee, Y., Triplett, K., Donkor, M., Lungu, O.I., Lux, A., Marshall, N., Lindorfer, M.A., Richard-Le Goff, O., Balbino, B., Kang, T.H., Tanno, H., Delidakis, G., Alford, C., Taylor, R.P., Nimmerjahn, F., Varadarajan, N., Bruhns, P. Zhang, Y.J. and G. Georgiou, “IgG Fc Domains that Bind C1q but not Effector Fcγ Receptors Delineate the Significance of Complement-Dependent Cell-Mediated Cytotoxicity and Phagocytosis in Antibody Function,” Nature Immunology 18:889-898 (2017) DOI: 10.1038/ni.3770
Lau, D., Lan, L.Y., Andrews, S.F., Henry, C., Thatcher Rojas, K., Neu, K.E., Huang, Y., DeKosky, B., Palm, A.E., Ippolito, G.C., Georgiou, G., Wilson, P., “Low CD21 expression defines a population of recent germinal center graduates primed for plasma cell differentiation” Science Immunology Vol. 2, Issue 7 (2017) DOI: 10.1126/sciimmunol.aai8153
Steiniger, S.C., Glanville, J., Harris, D.W., Wilson, T.L., Ippolito, G.C., and Dunham, S.A., “Comparative analysis of the feline immunoglobulin repertoire,” Biologicals 46:81-87 (2017) PMID: 28131552
Williams, L.D., Ofek, G., Schatzle, S., McDaniel, J.R., Lu, X., Nicely, N.I., Wu, L., Lougheed, C.S., Bradley, T., Louder, M.K., McKee, K., Bailer, R.T., O’Dell, S., Georgiev, I.S., Seaman, M., Parks, R., Marshall, D., Anasti, K., Yang, G., Nie, X., Tumab, N., Wiehe, K., Wagh, K., Korber, B., Kepler, T., Alam, S.M., Morris, L., Kamanga, G., Choen, M.S., Bonsignori, M., Xia, S., Montefiori, D., Kelsoe, G., Gao, F., Mascola, J.R., Moody, M.A., Saunders, K.O., Liao, H., Tomaras, G.D., Georgiou, G., Haynes, B.F., “Potent and broad HIV-neutralizing antibodies in memory B cells and plasma”, Science Immunology 27 Jan 2017: Vol. 2, Issue 7. DOI: 10.1126/sciimmunol.aal2200
Li, Q., Yi, L., Hoi, K.H., Marek, P., Georgiou, G. and Iverson, B.L., “Profiling Protease Specificity: Combining Yeast ER Sequestration Screening (YESS) with Next Generation Sequencing,” ACS Chem. Biol. (2017) PMID: 27977123
Cramer, S.L., Saha, A., Liu, J., Tadi, S., Tiziani, S., Yan, W., Triplett, K., Lamb, C., Alters, S.E., Rowlinson, S., Zhang, Y., Keating, M.J., Huang, P., DiGiovanni, J., Georgiou, G., and E. Stone, “Systemic Depletion of Serum L-Cyst(e)ine with an Engineering Human Enzyme Mediates Potent Induction of ROS Leading to Selective Cancer Ablation,” Nature Medicine 23, 120-127, doi: 10.1038/nm.4232 (2017) PMID: 27869804
- Commentaries and News Stories: Covered by >24 scientific and mainstream media publications; e.g. Cancer Discovery: http://cancerdiscovery.aacrjournals.org/content/early/2016/12/15/2159-8290.CD-RW2016-235 ; Yahoo Finance: http://finance.yahoo.com/news/study-published-nature-medicine-highlights-122000477.html
2021
Voss WN, Hou YJ, Johnson NV, Delidakis G, Kim JE, Javanmardi K, Horton AP, Bartzoka F, Paresi CJ, Tanno Y, Chou C-W, Abbasi SA, Pickens W, George K, Boutz DR, Towers DM, McDaniel JR, Billick D, Goike J, Rowe L, Batra D, Pohl J, Lee J, Gangappa S, Sambhara S, Gadush M, Wang N, Person MD, Iverson BL, Gollihar JD, Dye J, Herbert A, Finkelstein IJ, Baric RS, McLellan JS, Georgiou G, Lavinder JJ*, Ippolito GC*. Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes. Science (2021) 372(6546):1108-1112. doi: 10.1126/science.abg5268. PMID: 33947773.
McCarthy, K.R., Lee, J. Watanabe, A., Kuraoka, M., Robinson-McCarthy, L-R., Georgiou, G., Kelsoe, G., and Stephen C. Harrison, “A Prevalent Focused Human Antibody Response to the Influenza Hemagglutinin Head Interface,” mBio (2021) in press
Jung J, Mundle ST, Ustyugova IV, Horton AP, Boutz DR, Pougatcheva S, Prabakaran P, McDaniel JR, King GR, Park D, Person MD, Ye C, Tan B, Tanno Y, Kim JE, Curtis NC, DiNapoli J, Delagrave S, Ross TM, Ippolito GC, Kleanthous H, Lee J, Georgiou G. Influenza vaccination in the elderly boosts antibodies against conserved viral proteins and egg-produced glycans. J Clin Invest (2021) 131(13):e148763. doi: 10.1172/JCI148763. PMID: 34196304; PMCID: PMC8245176.
Li J, Bazzi SA, Schmitz F, Tanno H, McDaniel JR, Lee CH, Joshi C, Kim JE, Monson N, Greenberg BM, Hedfalk K, Melamed E*, Ippolito GC*. Molecular Level Characterization of Circulating Aquaporin-4 Antibodies in Neuromyelitis Optica Spectrum Disorder. Neurol Neuroimmunol Neuroinflamm (2021) 8(5):e1034. doi: 10.1212/NXI.0000000000001034. PMID: 34168058; PMCID: PMC8225010.
2020
Mehaffey M.R., Lee J., Jung J., Lanzillotti M.B., Escobar E.E., Morgenstern K.R., Georgiou G., Brodbelt J.S., “Mapping a Conformational Epitope of Hemagglutinin A Using Native Mass Spectrometry and Ultraviolet Photodissociation,” Anal Chem 92(17):11869-11878 (2020) PMID: 32867493 DOI: 10.1021/acs.analchem.0c02237.
Verardi, R., Lindesmith, L.C., Tsybovsky, Y., Gorman, J., Chuang, Y.-G., Edwards, K., Jensen, P.B., Mallory, M.L., Ou, L., Schön, A., Tully, E.S. Georgiou, G. Baric, R.S. and P.D. Kwong “Disulfide-Stabilization of Human Norovirus GI.1 Virus-like Particles Focuses Immune Response Toward Neutralizing Epitopes” NPJ Vaccines (2020) in press.
Lyu, A., Triplett, T.A., Nam, S.H., Hu, Z., Arasappan, D., Godfrey, H.D., Ames, R.Y., Sarang, A., Selden, H.J., Lee, C-H., Georgiou, G., Horton, T.M., Ehrlich, L.I.R. “Tumor-Associated Myeloid Cells Provide Critical Support for T-ALL”. Blood DOI: 10.1182/blood.2020007145 PMID: 32845007
Soni, C., Perez, O.A., Voss, W.N., Pucella, J.N., Serpas, L., Mehl, J., Ching, K.L., Goike, J., Georgiou, G.,
Ippolito, G.C., Sisirak, V. and Reizis, B. “Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA” Immunity 52:1022-1038 (2020) PMID: 32454024 DOI:10.1016/j.immuni.2020.04.015
Lu, W-C., Saha, A., Yan, W., Garrison, K., Lamb, C., Pandey, R., Irani, S., Lodi, A., Lu, X., Tiziani, S., Zhang., Y.J., Georgiou, G., DiGiovanni, J. and E. Stone, “Enzyme-Mediated Depletion of Serum L-Met Abrogates Prostate Cancer Growth via Multiple Mechanisms without Evidence of Systemic Toxicity, “Proc. Natl. Acad. Sci. USA 117:532-540 (2020) PMID: 32434918, DOI: 10.1073/pnas.1917362117
Badgley, M.A., Kremer, D., Maurer, H.C., DelGiorno, K.E., Lee, H-J., Purohit, H., Sagalovskiy, I., Ma, A., Kapillian, J., Firl, E.M., Decker, A.R., Sastra, S.A, Palermo, C.F., Andrade, L.R., Sajjakulnukit, P., Zhang, L., Tolstyka, Z.P., Hirschhorn, T., Lamb, C., Liu, T., Gu, W., Seeley, E.S., Stone, E., Georgiou, G., Manor, U., Iuga, A., Wahl, G. F., Stockwell, B.R., Lyssiotis, C.A. and K. P. Olive, “Cysteine depletion induces pancreatic tumor ferroptosis in mice, “ Science 368:85-89 (2020) PMID: 32241947, DOI: 10.1126/science.aaw9872
Tanno, H., McDaniel, J.R., Stevens, C.A., Voss, W.N., Li. J., Durrett, R., Lee, J., Gollihar, J., Tanno, Y., Delidakis, G., Pothukuchy, A. Ellefson, J.W., Goronzy, J.J., Maynard, J.A., Ellington, A.D., Ippolito, G.C. and G. Georgiou, “A Facile Technology for the High Throughput Sequencing of the Paired VH:VL and TCR:TCR Repertoires ,” Science Advances 6:eaay9093 (2020) PMID: 32426460, DOI: 10.1126/sciadv.aay9093.
Tanno, H., Gould, T.M., McDaniel, J.R., Cao,W., Tanno, Y., Durrett, R.E., Park, D., Cate, S.J., Hildebrand, W.H., Dekker, C.L., Tian, L., Weyand, C.M., Georgiou, G. and J.J., Goronzy, “Determinants Governing T cell Receptor α/β-Chain Pairing in Repertoire Formation of Identical Twins,” Proc. Natl. Acad. Sci. USA 117:532-540 (2020) PMID: 31879353, DOI: 10.1073/pnas.1915008117
Lee, J., Der, B.S., Karamitros, C.S., Li, W., Marshall, N.M., Lungu, O.A., Miklos , A.E., Xu , J., Kang, T-H., Lee, C-H., Tan, B., Hughes , R.A., Jung, S-T., Ippolito , G.C.. Gray, J.J., Zhang, Y., Kuhlman, B., Georgiou G. and A.D. Ellington, “Computer-Based Engineering of Thermostabilized Antibody Fragments,” AICHE J., 66: e16864 (2020) DOI: 10.1002/aic.16864
Sterrett S, Peng BJ, Burton RL, LaFon DC, Westfall AO, Singh S, Pride M, Anderson AS, Ippolito GC, Schroeder HW Jr, Nahm MH, Krishna Prasad A, Goepfert P, Bansal A. Peripheral CD4 T follicular cells induced by a conjugated pneumococcal vaccine correlate with enhanced opsonophagocytic antibody responses in younger individuals. Vaccine. 2020 Feb 11;38(7):1778-1786. doi: 10.1016/j.vaccine.2019.12.023. Epub 2020 Jan 3. PMID: 31911030.
2019
Dekker JD, Baracho GV, Zhu Z, Ippolito GC, Schmitz RJ, Rickert RC, Tucker HO. Loss of the FOXP1 Transcription Factor Leads to Deregulation of B Lymphocyte Development and Function at Multiple Stages. Immunohorizons. 2019 Oct 7;3(10):447-462. PMID: 31591252. DOI: 10.4049/immunohorizons.1800079.
Taft, J.M., Georgeon, S., Allen, C., Reckel, S., DeSautelle, J., Hantschel, O., Georgiou, G., Iverson, B.L., “Rapid Screen for Tyrosine Kinase Inhibitor Resistance Mutations and Substrate Specificity,” ACS Chem Biol. 14(9):1888-1895 (2019) PMID: 31339688, DOI: 10.1021/acschembio.9b00283.
Kshattry, S., Saha, A., Gries, P., Tiziani, S., Stone, EM., Georgiou, G., DiGiovanni, J., “Enzyme-mediated depletion of l-cyst(e)ine synergizes with thioredoxin reductase inhibition for suppression of pancreatic tumor growth,” NPJ Precis Oncol. (2019) eCollection 2019, PMID: 31231686. DOI: 10.1038/s41698-019-0088-z.
Lindesmith, L.C., McDaniel, J.R., Changela, A., Varardi, R., Kerr S.A., Constantini, V., Brewer-Jensen, P.D., Mallory, M.L., Voss, W.N., Boutz, D.R., Blazeck, J. J., Ippolito, G.C., Vinje, J., Kwong, P.D., Georgiou, G. and Baric, R. “Boosted Broad Binding/Blockade Antibodies Dominate the Serological Repertoire to Norovirus Vaccine” Immunity 50: 1530-1541(2019) PMID: 31216462 DOI: 10.1016/j.immuni.2019.05.007
Wang, W., Green, M., Choi, J.E., Gijón, M., Kennedy, P.D., Johnson, J.K., Liao, P., Lang, X., Kryczek, I., Sell, A., Xia, H., Zhou, J., Li, G., Li, J., Li, W., Wei, S., Vatan, L., Zhang, H., Szeliga, W., Gu, W., Liu, R., Lawrence, T.S., Lamb, C., Tanno, Y., Cieslik, M., Stone, E., Georgiou. G., Chan, T.A., Chinnaiyan, A. and W. Zou, “CD8+ T Cells Regulate Tumour Ferroptosis During Cancer Immunotherapy” Nature (2019) PMID: 31043744 DOI: 10.1038/s41586-019-1170-y
Kang, T.H., Lee, C.H., Delidakis, G., Jung, J., Richard-Le Goff, O., Lee, J., Kim, J.E., Charab, W., Bruhns, P. and G. Georgiou, “An Engineered Human Fc variant With Exquisite Selectivity for FcγRIIIaV158 Reveals That Ligation of FcγRIIIa Mediates Potent Antibody Dependent Cellular Phagocytosis With GM-CSF-Differentiated Macrophages,” Front Immunology 10:562 (2019) PMID: 30984171 DOI: 10.3389/fimmu.2019.00562
Krebs, S.J., Kwon, Y.D., Schramm, C.A., Law, W.H., Donofrio, G., Zhou, K.H., Gift, S., Dussupt, V., Georgiev, I.S., Schätzle, S., McDaniel, J.R., Lai, Y.T., Sastry, M., Zhang, B., Jarosinski, M.C., Ransier, A., Chenine, A.L., Asokan, M., Bailer, R.T., Bose, M., Cagigi, A., Cale, E.M., Chuang, G.Y., Darko, S., Driscoll, J.I., Druz, A., Gorman, J., Laboune, F., Louder, M.K., McKee, K., Mendez, L., Moody, M.A., O’Sullivan, A.M., Owen, C., Peng, D., Rawi, R., Sanders-Buell, E., Shen, C.H., Shiakolas, A.R., Stephens, T., Tsybovsky, Y., Tucker, C., Verardi, R., Wang, K., Zhou, J., Zhou, T., Georgiou, G., Alam, S.M., Haynes, B.F., Rolland, M., Matyas, G.R., Polonis, V.R., McDermott, A.B., Douek, D.C., Shapiro, L., Tovanabutra, S., Michael, N.L., Mascola, J.R., Robb, M.L., Kwong, P.D. and N.A. Doria-Rose, “Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual,” Immunity 50:677-691 (2019) PMID: 30876875 DOI: 10.1016/j.immuni.2019.02.008
Lee, J., Paparoditis, P., Horton, A.P., Frühwirth, A., McDaniel, J.R., Jung, J., Boutz, D.R., Hussein, D.A., Tanno, Y., Pappas, L., Ippolito, G.C., Corti, D., Lanzavecchia, A. and G. Georgiou, “Persistent Antibody Clonotypes Dominate the Serum Response to Influenza over Multiple Years and Repeated Vaccinations,” Cell Host Microbe 25:367-376 (2019) PMID: 30795981 DOI: 10.1016/j.chom.2019.01.010
Manso, T.C., Groenner-Penna, M., Minozzo, J.C., Antunes, B.C., Ippolito, G.C., Molina, F., and Felicori, L.F., “Next-generation sequencing reveals new insights about gene usage and CDR-H3 composition in the horse antibody repertoire,” Mol. Immunology 105:251-259 (2019) PMID: 30562645
2018
Johnson, E.L., Doria-Rose, N.A., Gorman, J., Bhiman, J.N., Schramm, C.A., Vu, A.Q., Law, W.H., Zhang, B., Bekker, V., Karim, S.S.A., Ippolito, G.C., Morris, L., Moore, P.L. Kwong, P.D., Mascola, J.R. and G. Georgiou, “Sequencing HIV-neutralizing antibody exons and introns reveals detailed aspects of lineage maturation” Nature Communications 9(1):4136 (2018) PMID: 30297708 DOI:10.1038/s41467-018-06424-6
Saphire, E.O., Schendel, S.L., Fusco, M.L., Gangavarapu, K., Gunn, B.M., Wec, A.Z., Halfmann, P.J., Brannan, J.M., Herbert, A.S., Qiu, X., Wagh, K., He, S., Giorgi, E.E., Theiler J, Pommert KBJ, Krause TB, Turner HL, Murin CD, Pallesen J, Davidson E, Ahmed R, Aman MJ, Bukreyev A, Burton DR, Crowe JE Jr, Davis CW, Georgiou G, Krammer F, Kyratsous CA, Lai JR, Nykiforuk C, Pauly MH, Rijal P, Takada A, Townsend AR, Volchkov V, Walker LM, Wang CI, Zeitlin L, Doranz BJ, Ward AB, Korber B, Kobinger GP, Andersen KG, Kawaoka Y, Alter G, Chandran K, Dye JM; Viral Hemorrhagic Fever Immunotherapeutic Consortium, “Systematic Analysis of Monoclonal Antibodies against Ebola Virus GP Defines Features that Contribute to Protection,” Cell 2018 Aug 9;174(4):938-952.e13. doi: 10.1016/j.cell.2018.07.033. PMID: 30096313
Triplett, T.A., Garrison, K.C., Marshall, N., Donkor, M., Blazeck, J., Lamb, C., Qerqez A., Dekker, J.D., Tanno, Y. Lu, W-C., Karamitros, S.K., Ford, K., Tan, B., Zhang, M., McGovern, K., Coma, S., Kumada, Y., Yamany, M.S., Sentandreu, S., Fromm, G., Tiziani, S., Schreiber, T.H., Manfredi, M., Ehrlich, L.I.R., Stone E., and G. Georgiou, “Reversal of IDO-Mediated Cancer Immune Suppression by Systemic Kynurenine Depletion with a Therapeutic Enzyme,” Nature Biotechnology doi: 10.1038/nbt.4180
Lee, J. and G. Georgiou, “High Affinity IgA Against Microbial Antigens” Nature Immunology 2018 19:514-515 (2018) PMID: 29777208
Liu, Y., McDaniel, J.R., Khan, S., Campisi, P., Propst, E.J., Holler, T., Grunebaum, E., Georgiou, G., Ippolito, G.C. and Ehrhardt, G.R.A., “Antibodies Encoded by FCRL4-Bearing Memory B Cells Preferentially Recognize Commensal Microbial Antigens,” J Immunology 200:3962-3969 (2018) PMID: 29703863
Chen, Y.Q., Wohlbold, T.J., Zheng, N.Y., Huang, M., Huang, Y., Neu, K.E., Lee, J., Wan, H., Rojas. K.T., Kirkpatrick., E., Henry, C., Palm, A.E., Stamper, C.T., Lan, L.Y., Topham, D.J., Treanor, J., Wrammert, J., Ahmed, R., Eichelberger, M.C., Georgiou, G, Krammer, F. and Wilson, P.C., “Influenza Infection in Humans Induces Broadly Cross-Reactive and Protective Neuraminidase-Reactive Antibodies,” Cell 173:417-429 (2018). PMID: 29625056
- Commentaries and News Stories: Covered by 9 scientific and mainstream media publications
McDaniel, J.R., Pero, S.C., Voss, W.N., Shukla, G.S., Sun, Y., Schaetzle, S., Lee, C.H., Horton, A.P., Harlow, S., Gollihar, J., Ellefson, J.W., Krag, C.C., Tanno, Y., Sidiropoulos, N., Georgiou, G., Ippolito, G.C. and Krag, D.N., “Identification of Tumor-Reactive B Cells and Systemic IgG in Breast Cancer Based on Clonal Frequency in the Sentinel Lymph Node,” Cancer Immunology Immunother 67:729-738 (2018) PMID: 29427082
Wang, B., DeKosky, B.J., Timm, M.R., Lee, J., Normandin, E., Misasi, J., Kong, R., McDaniel, J.R., Delidakis, G., Leigh, K.E., Niezold, T., Choi, C.W., Viox, E.G., Fahad, A., Cagigi, A., Ploquin, A., Leung, K., Yang, E.S., Kong, W.P., Voss, W.N., Schmidt, A.G., Moody, M.A., Ambrozak, D.R., Henry, A.R., Laboune, F., Ledgerwood, J.E., Graham, B.S., Connors, M., Douek, D.C., Sullivan, N.J., Ellington, A.D., Mascola, J.R. and G. Georgiou, “Functional Interrogation and Mining of Natively Paired Human VH:VL Antibody Repertoires,” Nature Biotechnology 36:152-155 (2018) PMID: 29309060
2017
McDaniel, J.R., Ippolito G.C. and G. Georgiou, “Mapping the Secrets of the Antibody Pool,” Nature Biotechnology 35:921-922. (2017) PMID: 28892077.
Jang, S-H., Gregersen, P.K., Georgiou, G., Diamond, B., “Increased Cathepsin S in Dendritic Cells Alters TFH Repertoire and Contributes to Lupus,”, Nature Immunology 18:1016-1024 (2017) PMID: 28692065
Cotham, V.C., Horton, A.P., Lee, J., Georgiou, G. and Brodbelt, J.S., “Middle-down 193-nm Ultraviolet Photodissociation for Unambiguous Antibody Identification and its Implications for Immunoproteomic Analysis,” Anal. Chem. 89:6498-6504 (2017) PMID: 28517930
Lee, C.-H., Romain, G., Yan, W., Watanabe, W., Charab, W., Todorova, B., Lee, Y., Triplett, K., Donkor, M., Lungu, O.I., Lux, A., Marshall, N., Lindorfer, M.A., Richard-Le Goff, O., Balbino, B., Kang, T.H., Tanno, H., Delidakis, G., Alford, C., Taylor, R.P., Nimmerjahn, F., Varadarajan, N., Bruhns, P. Zhang, Y.J. and G. Georgiou, “IgG Fc Domains that Bind C1q but not Effector Fcγ Receptors Delineate the Significance of Complement-Dependent Cell-Mediated Cytotoxicity and Phagocytosis in Antibody Function,” Nature Immunology 18:889-898 (2017) DOI: 10.1038/ni.3770
Lau, D., Lan, L.Y., Andrews, S.F., Henry, C., Thatcher Rojas, K., Neu, K.E., Huang, Y., DeKosky, B., Palm, A.E., Ippolito, G.C., Georgiou, G., Wilson, P., “Low CD21 expression defines a population of recent germinal center graduates primed for plasma cell differentiation” Science Immunology Vol. 2, Issue 7 (2017) DOI: 10.1126/sciimmunol.aai8153
Steiniger, S.C., Glanville, J., Harris, D.W., Wilson, T.L., Ippolito, G.C., and Dunham, S.A., “Comparative analysis of the feline immunoglobulin repertoire,” Biologicals 46:81-87 (2017) PMID: 28131552
Williams, L.D., Ofek, G., Schatzle, S., McDaniel, J.R., Lu, X., Nicely, N.I., Wu, L., Lougheed, C.S., Bradley, T., Louder, M.K., McKee, K., Bailer, R.T., O’Dell, S., Georgiev, I.S., Seaman, M., Parks, R., Marshall, D., Anasti, K., Yang, G., Nie, X., Tumab, N., Wiehe, K., Wagh, K., Korber, B., Kepler, T., Alam, S.M., Morris, L., Kamanga, G., Choen, M.S., Bonsignori, M., Xia, S., Montefiori, D., Kelsoe, G., Gao, F., Mascola, J.R., Moody, M.A., Saunders, K.O., Liao, H., Tomaras, G.D., Georgiou, G., Haynes, B.F., “Potent and broad HIV-neutralizing antibodies in memory B cells and plasma”, Science Immunology 27 Jan 2017: Vol. 2, Issue 7. DOI: 10.1126/sciimmunol.aal2200
Li, Q., Yi, L., Hoi, K.H., Marek, P., Georgiou, G. and Iverson, B.L., “Profiling Protease Specificity: Combining Yeast ER Sequestration Screening (YESS) with Next Generation Sequencing,” ACS Chem. Biol. (2017) PMID: 27977123
Cramer, S.L., Saha, A., Liu, J., Tadi, S., Tiziani, S., Yan, W., Triplett, K., Lamb, C., Alters, S.E., Rowlinson, S., Zhang, Y., Keating, M.J., Huang, P., DiGiovanni, J., Georgiou, G., and E. Stone, “Systemic Depletion of Serum L-Cyst(e)ine with an Engineering Human Enzyme Mediates Potent Induction of ROS Leading to Selective Cancer Ablation,” Nature Medicine 23, 120-127, doi: 10.1038/nm.4232 (2017) PMID: 27869804
- Commentaries and News Stories: Covered by >24 scientific and mainstream media publications; e.g. Cancer Discovery: http://cancerdiscovery.aacrjournals.org/content/early/2016/12/15/2159-8290.CD-RW2016-235 ; Yahoo Finance: http://finance.yahoo.com/news/study-published-nature-medicine-highlights-122000477.html
2021
Voss WN, Hou YJ, Johnson NV, Delidakis G, Kim JE, Javanmardi K, Horton AP, Bartzoka F, Paresi CJ, Tanno Y, Chou C-W, Abbasi SA, Pickens W, George K, Boutz DR, Towers DM, McDaniel JR, Billick D, Goike J, Rowe L, Batra D, Pohl J, Lee J, Gangappa S, Sambhara S, Gadush M, Wang N, Person MD, Iverson BL, Gollihar JD, Dye J, Herbert A, Finkelstein IJ, Baric RS, McLellan JS, Georgiou G, Lavinder JJ*, Ippolito GC*. Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes. Science (2021) 372(6546):1108-1112. doi: 10.1126/science.abg5268. PMID: 33947773.
McCarthy, K.R., Lee, J. Watanabe, A., Kuraoka, M., Robinson-McCarthy, L-R., Georgiou, G., Kelsoe, G., and Stephen C. Harrison, “A Prevalent Focused Human Antibody Response to the Influenza Hemagglutinin Head Interface,” mBio (2021) in press
Jung J, Mundle ST, Ustyugova IV, Horton AP, Boutz DR, Pougatcheva S, Prabakaran P, McDaniel JR, King GR, Park D, Person MD, Ye C, Tan B, Tanno Y, Kim JE, Curtis NC, DiNapoli J, Delagrave S, Ross TM, Ippolito GC, Kleanthous H, Lee J, Georgiou G. Influenza vaccination in the elderly boosts antibodies against conserved viral proteins and egg-produced glycans. J Clin Invest (2021) 131(13):e148763. doi: 10.1172/JCI148763. PMID: 34196304; PMCID: PMC8245176.
Li J, Bazzi SA, Schmitz F, Tanno H, McDaniel JR, Lee CH, Joshi C, Kim JE, Monson N, Greenberg BM, Hedfalk K, Melamed E*, Ippolito GC*. Molecular Level Characterization of Circulating Aquaporin-4 Antibodies in Neuromyelitis Optica Spectrum Disorder. Neurol Neuroimmunol Neuroinflamm (2021) 8(5):e1034. doi: 10.1212/NXI.0000000000001034. PMID: 34168058; PMCID: PMC8225010.
2020
Mehaffey M.R., Lee J., Jung J., Lanzillotti M.B., Escobar E.E., Morgenstern K.R., Georgiou G., Brodbelt J.S., “Mapping a Conformational Epitope of Hemagglutinin A Using Native Mass Spectrometry and Ultraviolet Photodissociation,” Anal Chem 92(17):11869-11878 (2020) PMID: 32867493 DOI: 10.1021/acs.analchem.0c02237.
Verardi, R., Lindesmith, L.C., Tsybovsky, Y., Gorman, J., Chuang, Y.-G., Edwards, K., Jensen, P.B., Mallory, M.L., Ou, L., Schön, A., Tully, E.S. Georgiou, G. Baric, R.S. and P.D. Kwong “Disulfide-Stabilization of Human Norovirus GI.1 Virus-like Particles Focuses Immune Response Toward Neutralizing Epitopes” NPJ Vaccines (2020) in press.
Lyu, A., Triplett, T.A., Nam, S.H., Hu, Z., Arasappan, D., Godfrey, H.D., Ames, R.Y., Sarang, A., Selden, H.J., Lee, C-H., Georgiou, G., Horton, T.M., Ehrlich, L.I.R. “Tumor-Associated Myeloid Cells Provide Critical Support for T-ALL”. Blood DOI: 10.1182/blood.2020007145 PMID: 32845007
Soni, C., Perez, O.A., Voss, W.N., Pucella, J.N., Serpas, L., Mehl, J., Ching, K.L., Goike, J., Georgiou, G.,
Ippolito, G.C., Sisirak, V. and Reizis, B. “Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA” Immunity 52:1022-1038 (2020) PMID: 32454024 DOI:10.1016/j.immuni.2020.04.015
Lu, W-C., Saha, A., Yan, W., Garrison, K., Lamb, C., Pandey, R., Irani, S., Lodi, A., Lu, X., Tiziani, S., Zhang., Y.J., Georgiou, G., DiGiovanni, J. and E. Stone, “Enzyme-Mediated Depletion of Serum L-Met Abrogates Prostate Cancer Growth via Multiple Mechanisms without Evidence of Systemic Toxicity, “Proc. Natl. Acad. Sci. USA 117:532-540 (2020) PMID: 32434918, DOI: 10.1073/pnas.1917362117
Badgley, M.A., Kremer, D., Maurer, H.C., DelGiorno, K.E., Lee, H-J., Purohit, H., Sagalovskiy, I., Ma, A., Kapillian, J., Firl, E.M., Decker, A.R., Sastra, S.A, Palermo, C.F., Andrade, L.R., Sajjakulnukit, P., Zhang, L., Tolstyka, Z.P., Hirschhorn, T., Lamb, C., Liu, T., Gu, W., Seeley, E.S., Stone, E., Georgiou, G., Manor, U., Iuga, A., Wahl, G. F., Stockwell, B.R., Lyssiotis, C.A. and K. P. Olive, “Cysteine depletion induces pancreatic tumor ferroptosis in mice, “ Science 368:85-89 (2020) PMID: 32241947, DOI: 10.1126/science.aaw9872
Tanno, H., McDaniel, J.R., Stevens, C.A., Voss, W.N., Li. J., Durrett, R., Lee, J., Gollihar, J., Tanno, Y., Delidakis, G., Pothukuchy, A. Ellefson, J.W., Goronzy, J.J., Maynard, J.A., Ellington, A.D., Ippolito, G.C. and G. Georgiou, “A Facile Technology for the High Throughput Sequencing of the Paired VH:VL and TCR:TCR Repertoires ,” Science Advances 6:eaay9093 (2020) PMID: 32426460, DOI: 10.1126/sciadv.aay9093.
Tanno, H., Gould, T.M., McDaniel, J.R., Cao,W., Tanno, Y., Durrett, R.E., Park, D., Cate, S.J., Hildebrand, W.H., Dekker, C.L., Tian, L., Weyand, C.M., Georgiou, G. and J.J., Goronzy, “Determinants Governing T cell Receptor α/β-Chain Pairing in Repertoire Formation of Identical Twins,” Proc. Natl. Acad. Sci. USA 117:532-540 (2020) PMID: 31879353, DOI: 10.1073/pnas.1915008117
Lee, J., Der, B.S., Karamitros, C.S., Li, W., Marshall, N.M., Lungu, O.A., Miklos , A.E., Xu , J., Kang, T-H., Lee, C-H., Tan, B., Hughes , R.A., Jung, S-T., Ippolito , G.C.. Gray, J.J., Zhang, Y., Kuhlman, B., Georgiou G. and A.D. Ellington, “Computer-Based Engineering of Thermostabilized Antibody Fragments,” AICHE J., 66: e16864 (2020) DOI: 10.1002/aic.16864
Sterrett S, Peng BJ, Burton RL, LaFon DC, Westfall AO, Singh S, Pride M, Anderson AS, Ippolito GC, Schroeder HW Jr, Nahm MH, Krishna Prasad A, Goepfert P, Bansal A. Peripheral CD4 T follicular cells induced by a conjugated pneumococcal vaccine correlate with enhanced opsonophagocytic antibody responses in younger individuals. Vaccine. 2020 Feb 11;38(7):1778-1786. doi: 10.1016/j.vaccine.2019.12.023. Epub 2020 Jan 3. PMID: 31911030.
2019
Dekker JD, Baracho GV, Zhu Z, Ippolito GC, Schmitz RJ, Rickert RC, Tucker HO. Loss of the FOXP1 Transcription Factor Leads to Deregulation of B Lymphocyte Development and Function at Multiple Stages. Immunohorizons. 2019 Oct 7;3(10):447-462. PMID: 31591252. DOI: 10.4049/immunohorizons.1800079.
Taft, J.M., Georgeon, S., Allen, C., Reckel, S., DeSautelle, J., Hantschel, O., Georgiou, G., Iverson, B.L., “Rapid Screen for Tyrosine Kinase Inhibitor Resistance Mutations and Substrate Specificity,” ACS Chem Biol. 14(9):1888-1895 (2019) PMID: 31339688, DOI: 10.1021/acschembio.9b00283.
Kshattry, S., Saha, A., Gries, P., Tiziani, S., Stone, EM., Georgiou, G., DiGiovanni, J., “Enzyme-mediated depletion of l-cyst(e)ine synergizes with thioredoxin reductase inhibition for suppression of pancreatic tumor growth,” NPJ Precis Oncol. (2019) eCollection 2019, PMID: 31231686. DOI: 10.1038/s41698-019-0088-z.
Lindesmith, L.C., McDaniel, J.R., Changela, A., Varardi, R., Kerr S.A., Constantini, V., Brewer-Jensen, P.D., Mallory, M.L., Voss, W.N., Boutz, D.R., Blazeck, J. J., Ippolito, G.C., Vinje, J., Kwong, P.D., Georgiou, G. and Baric, R. “Boosted Broad Binding/Blockade Antibodies Dominate the Serological Repertoire to Norovirus Vaccine” Immunity 50: 1530-1541(2019) PMID: 31216462 DOI: 10.1016/j.immuni.2019.05.007
Wang, W., Green, M., Choi, J.E., Gijón, M., Kennedy, P.D., Johnson, J.K., Liao, P., Lang, X., Kryczek, I., Sell, A., Xia, H., Zhou, J., Li, G., Li, J., Li, W., Wei, S., Vatan, L., Zhang, H., Szeliga, W., Gu, W., Liu, R., Lawrence, T.S., Lamb, C., Tanno, Y., Cieslik, M., Stone, E., Georgiou. G., Chan, T.A., Chinnaiyan, A. and W. Zou, “CD8+ T Cells Regulate Tumour Ferroptosis During Cancer Immunotherapy” Nature (2019) PMID: 31043744 DOI: 10.1038/s41586-019-1170-y
Kang, T.H., Lee, C.H., Delidakis, G., Jung, J., Richard-Le Goff, O., Lee, J., Kim, J.E., Charab, W., Bruhns, P. and G. Georgiou, “An Engineered Human Fc variant With Exquisite Selectivity for FcγRIIIaV158 Reveals That Ligation of FcγRIIIa Mediates Potent Antibody Dependent Cellular Phagocytosis With GM-CSF-Differentiated Macrophages,” Front Immunology 10:562 (2019) PMID: 30984171 DOI: 10.3389/fimmu.2019.00562
Krebs, S.J., Kwon, Y.D., Schramm, C.A., Law, W.H., Donofrio, G., Zhou, K.H., Gift, S., Dussupt, V., Georgiev, I.S., Schätzle, S., McDaniel, J.R., Lai, Y.T., Sastry, M., Zhang, B., Jarosinski, M.C., Ransier, A., Chenine, A.L., Asokan, M., Bailer, R.T., Bose, M., Cagigi, A., Cale, E.M., Chuang, G.Y., Darko, S., Driscoll, J.I., Druz, A., Gorman, J., Laboune, F., Louder, M.K., McKee, K., Mendez, L., Moody, M.A., O’Sullivan, A.M., Owen, C., Peng, D., Rawi, R., Sanders-Buell, E., Shen, C.H., Shiakolas, A.R., Stephens, T., Tsybovsky, Y., Tucker, C., Verardi, R., Wang, K., Zhou, J., Zhou, T., Georgiou, G., Alam, S.M., Haynes, B.F., Rolland, M., Matyas, G.R., Polonis, V.R., McDermott, A.B., Douek, D.C., Shapiro, L., Tovanabutra, S., Michael, N.L., Mascola, J.R., Robb, M.L., Kwong, P.D. and N.A. Doria-Rose, “Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual,” Immunity 50:677-691 (2019) PMID: 30876875 DOI: 10.1016/j.immuni.2019.02.008
Lee, J., Paparoditis, P., Horton, A.P., Frühwirth, A., McDaniel, J.R., Jung, J., Boutz, D.R., Hussein, D.A., Tanno, Y., Pappas, L., Ippolito, G.C., Corti, D., Lanzavecchia, A. and G. Georgiou, “Persistent Antibody Clonotypes Dominate the Serum Response to Influenza over Multiple Years and Repeated Vaccinations,” Cell Host Microbe 25:367-376 (2019) PMID: 30795981 DOI: 10.1016/j.chom.2019.01.010
Manso, T.C., Groenner-Penna, M., Minozzo, J.C., Antunes, B.C., Ippolito, G.C., Molina, F., and Felicori, L.F., “Next-generation sequencing reveals new insights about gene usage and CDR-H3 composition in the horse antibody repertoire,” Mol. Immunology 105:251-259 (2019) PMID: 30562645
2018
Johnson, E.L., Doria-Rose, N.A., Gorman, J., Bhiman, J.N., Schramm, C.A., Vu, A.Q., Law, W.H., Zhang, B., Bekker, V., Karim, S.S.A., Ippolito, G.C., Morris, L., Moore, P.L. Kwong, P.D., Mascola, J.R. and G. Georgiou, “Sequencing HIV-neutralizing antibody exons and introns reveals detailed aspects of lineage maturation” Nature Communications 9(1):4136 (2018) PMID: 30297708 DOI:10.1038/s41467-018-06424-6
Saphire, E.O., Schendel, S.L., Fusco, M.L., Gangavarapu, K., Gunn, B.M., Wec, A.Z., Halfmann, P.J., Brannan, J.M., Herbert, A.S., Qiu, X., Wagh, K., He, S., Giorgi, E.E., Theiler J, Pommert KBJ, Krause TB, Turner HL, Murin CD, Pallesen J, Davidson E, Ahmed R, Aman MJ, Bukreyev A, Burton DR, Crowe JE Jr, Davis CW, Georgiou G, Krammer F, Kyratsous CA, Lai JR, Nykiforuk C, Pauly MH, Rijal P, Takada A, Townsend AR, Volchkov V, Walker LM, Wang CI, Zeitlin L, Doranz BJ, Ward AB, Korber B, Kobinger GP, Andersen KG, Kawaoka Y, Alter G, Chandran K, Dye JM; Viral Hemorrhagic Fever Immunotherapeutic Consortium, “Systematic Analysis of Monoclonal Antibodies against Ebola Virus GP Defines Features that Contribute to Protection,” Cell 2018 Aug 9;174(4):938-952.e13. doi: 10.1016/j.cell.2018.07.033. PMID: 30096313
Triplett, T.A., Garrison, K.C., Marshall, N., Donkor, M., Blazeck, J., Lamb, C., Qerqez A., Dekker, J.D., Tanno, Y. Lu, W-C., Karamitros, S.K., Ford, K., Tan, B., Zhang, M., McGovern, K., Coma, S., Kumada, Y., Yamany, M.S., Sentandreu, S., Fromm, G., Tiziani, S., Schreiber, T.H., Manfredi, M., Ehrlich, L.I.R., Stone E., and G. Georgiou, “Reversal of IDO-Mediated Cancer Immune Suppression by Systemic Kynurenine Depletion with a Therapeutic Enzyme,” Nature Biotechnology doi: 10.1038/nbt.4180
Lee, J. and G. Georgiou, “High Affinity IgA Against Microbial Antigens” Nature Immunology 2018 19:514-515 (2018) PMID: 29777208
Liu, Y., McDaniel, J.R., Khan, S., Campisi, P., Propst, E.J., Holler, T., Grunebaum, E., Georgiou, G., Ippolito, G.C. and Ehrhardt, G.R.A., “Antibodies Encoded by FCRL4-Bearing Memory B Cells Preferentially Recognize Commensal Microbial Antigens,” J Immunology 200:3962-3969 (2018) PMID: 29703863
Chen, Y.Q., Wohlbold, T.J., Zheng, N.Y., Huang, M., Huang, Y., Neu, K.E., Lee, J., Wan, H., Rojas. K.T., Kirkpatrick., E., Henry, C., Palm, A.E., Stamper, C.T., Lan, L.Y., Topham, D.J., Treanor, J., Wrammert, J., Ahmed, R., Eichelberger, M.C., Georgiou, G, Krammer, F. and Wilson, P.C., “Influenza Infection in Humans Induces Broadly Cross-Reactive and Protective Neuraminidase-Reactive Antibodies,” Cell 173:417-429 (2018). PMID: 29625056
- Commentaries and News Stories: Covered by 9 scientific and mainstream media publications
McDaniel, J.R., Pero, S.C., Voss, W.N., Shukla, G.S., Sun, Y., Schaetzle, S., Lee, C.H., Horton, A.P., Harlow, S., Gollihar, J., Ellefson, J.W., Krag, C.C., Tanno, Y., Sidiropoulos, N., Georgiou, G., Ippolito, G.C. and Krag, D.N., “Identification of Tumor-Reactive B Cells and Systemic IgG in Breast Cancer Based on Clonal Frequency in the Sentinel Lymph Node,” Cancer Immunology Immunother 67:729-738 (2018) PMID: 29427082
Wang, B., DeKosky, B.J., Timm, M.R., Lee, J., Normandin, E., Misasi, J., Kong, R., McDaniel, J.R., Delidakis, G., Leigh, K.E., Niezold, T., Choi, C.W., Viox, E.G., Fahad, A., Cagigi, A., Ploquin, A., Leung, K., Yang, E.S., Kong, W.P., Voss, W.N., Schmidt, A.G., Moody, M.A., Ambrozak, D.R., Henry, A.R., Laboune, F., Ledgerwood, J.E., Graham, B.S., Connors, M., Douek, D.C., Sullivan, N.J., Ellington, A.D., Mascola, J.R. and G. Georgiou, “Functional Interrogation and Mining of Natively Paired Human VH:VL Antibody Repertoires,” Nature Biotechnology 36:152-155 (2018) PMID: 29309060
2017
McDaniel, J.R., Ippolito G.C. and G. Georgiou, “Mapping the Secrets of the Antibody Pool,” Nature Biotechnology 35:921-922. (2017) PMID: 28892077.
Jang, S-H., Gregersen, P.K., Georgiou, G., Diamond, B., “Increased Cathepsin S in Dendritic Cells Alters TFH Repertoire and Contributes to Lupus,”, Nature Immunology 18:1016-1024 (2017) PMID: 28692065
Cotham, V.C., Horton, A.P., Lee, J., Georgiou, G. and Brodbelt, J.S., “Middle-down 193-nm Ultraviolet Photodissociation for Unambiguous Antibody Identification and its Implications for Immunoproteomic Analysis,” Anal. Chem. 89:6498-6504 (2017) PMID: 28517930
Lee, C.-H., Romain, G., Yan, W., Watanabe, W., Charab, W., Todorova, B., Lee, Y., Triplett, K., Donkor, M., Lungu, O.I., Lux, A., Marshall, N., Lindorfer, M.A., Richard-Le Goff, O., Balbino, B., Kang, T.H., Tanno, H., Delidakis, G., Alford, C., Taylor, R.P., Nimmerjahn, F., Varadarajan, N., Bruhns, P. Zhang, Y.J. and G. Georgiou, “IgG Fc Domains that Bind C1q but not Effector Fcγ Receptors Delineate the Significance of Complement-Dependent Cell-Mediated Cytotoxicity and Phagocytosis in Antibody Function,” Nature Immunology 18:889-898 (2017) DOI: 10.1038/ni.3770
Lau, D., Lan, L.Y., Andrews, S.F., Henry, C., Thatcher Rojas, K., Neu, K.E., Huang, Y., DeKosky, B., Palm, A.E., Ippolito, G.C., Georgiou, G., Wilson, P., “Low CD21 expression defines a population of recent germinal center graduates primed for plasma cell differentiation” Science Immunology Vol. 2, Issue 7 (2017) DOI: 10.1126/sciimmunol.aai8153
Steiniger, S.C., Glanville, J., Harris, D.W., Wilson, T.L., Ippolito, G.C., and Dunham, S.A., “Comparative analysis of the feline immunoglobulin repertoire,” Biologicals 46:81-87 (2017) PMID: 28131552
Williams, L.D., Ofek, G., Schatzle, S., McDaniel, J.R., Lu, X., Nicely, N.I., Wu, L., Lougheed, C.S., Bradley, T., Louder, M.K., McKee, K., Bailer, R.T., O’Dell, S., Georgiev, I.S., Seaman, M., Parks, R., Marshall, D., Anasti, K., Yang, G., Nie, X., Tumab, N., Wiehe, K., Wagh, K., Korber, B., Kepler, T., Alam, S.M., Morris, L., Kamanga, G., Choen, M.S., Bonsignori, M., Xia, S., Montefiori, D., Kelsoe, G., Gao, F., Mascola, J.R., Moody, M.A., Saunders, K.O., Liao, H., Tomaras, G.D., Georgiou, G., Haynes, B.F., “Potent and broad HIV-neutralizing antibodies in memory B cells and plasma”, Science Immunology 27 Jan 2017: Vol. 2, Issue 7. DOI: 10.1126/sciimmunol.aal2200
Li, Q., Yi, L., Hoi, K.H., Marek, P., Georgiou, G. and Iverson, B.L., “Profiling Protease Specificity: Combining Yeast ER Sequestration Screening (YESS) with Next Generation Sequencing,” ACS Chem. Biol. (2017) PMID: 27977123
Cramer, S.L., Saha, A., Liu, J., Tadi, S., Tiziani, S., Yan, W., Triplett, K., Lamb, C., Alters, S.E., Rowlinson, S., Zhang, Y., Keating, M.J., Huang, P., DiGiovanni, J., Georgiou, G., and E. Stone, “Systemic Depletion of Serum L-Cyst(e)ine with an Engineering Human Enzyme Mediates Potent Induction of ROS Leading to Selective Cancer Ablation,” Nature Medicine 23, 120-127, doi: 10.1038/nm.4232 (2017) PMID: 27869804
- Commentaries and News Stories: Covered by >24 scientific and mainstream media publications; e.g. Cancer Discovery: http://cancerdiscovery.aacrjournals.org/content/early/2016/12/15/2159-8290.CD-RW2016-235 ; Yahoo Finance: http://finance.yahoo.com/news/study-published-nature-medicine-highlights-122000477.html
2019
Taft, J.M., Georgeon, S., Allen, C., Reckel, S., DeSautelle, J., Hantschel, O., Georgiou, G., Iverson, B.L., “Rapid Screen for Tyrosine Kinase Inhibitor Resistance Mutations and Substrate Specificity,” ACS Chem Biol. 14(9):1888-1895 (2019) PMID: 31339688, DOI: 10.1021/acschembio.9b00283.
Kshattry, S., Saha, A., Gries, P., Tiziani, S., Stone, EM., Georgiou, G., DiGiovanni, J., “Enzyme-mediated depletion of l-cyst(e)ine synergizes with thioredoxin reductase inhibition for suppression of pancreatic tumor growth,” NPJ Precis Oncol. (2019) eCollection 2019, PMID: 31231686. DOI: 10.1038/s41698-019-0088-z.
Lindesmith, L.C., McDaniel, J.R., Changela, A., Varardi, R., Kerr S.A., Constantini, V., Brewer-Jensen, P.D., Mallory, M.L., Voss, W.N., Boutz, D.R., Blazeck, J. J., Ippolito, G.C., Vinje, J., Kwong, P.D., Georgiou, G. and Baric, R. “Boosted Broad Binding/Blockade Antibodies Dominate the Serological Repertoire to Norovirus Vaccine” Immunity 50: 1530-1541(2019) PMID: 31216462 DOI: 10.1016/j.immuni.2019.05.007
Wang, W., Green, M., Choi, J.E., Gijón, M., Kennedy, P.D., Johnson, J.K., Liao, P., Lang, X., Kryczek, I., Sell, A., Xia, H., Zhou, J., Li, G., Li, J., Li, W., Wei, S., Vatan, L., Zhang, H., Szeliga, W., Gu, W., Liu, R., Lawrence, T.S., Lamb, C., Tanno, Y., Cieslik, M., Stone, E., Georgiou. G., Chan, T.A., Chinnaiyan, A. and W. Zou, “CD8+ T Cells Regulate Tumour Ferroptosis During Cancer Immunotherapy” Nature (2019) PMID: 31043744 DOI: 10.1038/s41586-019-1170-y
Kang, T.H., Lee, C.H., Delidakis, G., Jung, J., Richard-Le Goff, O., Lee, J., Kim, J.E., Charab, W., Bruhns, P. and G. Georgiou, “An Engineered Human Fc variant With Exquisite Selectivity for FcγRIIIaV158 Reveals That Ligation of FcγRIIIa Mediates Potent Antibody Dependent Cellular Phagocytosis With GM-CSF-Differentiated Macrophages,” Front Immunology 10:562 (2019) PMID: 30984171 DOI: 10.3389/fimmu.2019.00562
Krebs, S.J., Kwon, Y.D., Schramm, C.A., Law, W.H., Donofrio, G., Zhou, K.H., Gift, S., Dussupt, V., Georgiev, I.S., Schätzle, S., McDaniel, J.R., Lai, Y.T., Sastry, M., Zhang, B., Jarosinski, M.C., Ransier, A., Chenine, A.L., Asokan, M., Bailer, R.T., Bose, M., Cagigi, A., Cale, E.M., Chuang, G.Y., Darko, S., Driscoll, J.I., Druz, A., Gorman, J., Laboune, F., Louder, M.K., McKee, K., Mendez, L., Moody, M.A., O’Sullivan, A.M., Owen, C., Peng, D., Rawi, R., Sanders-Buell, E., Shen, C.H., Shiakolas, A.R., Stephens, T., Tsybovsky, Y., Tucker, C., Verardi, R., Wang, K., Zhou, J., Zhou, T., Georgiou, G., Alam, S.M., Haynes, B.F., Rolland, M., Matyas, G.R., Polonis, V.R., McDermott, A.B., Douek, D.C., Shapiro, L., Tovanabutra, S., Michael, N.L., Mascola, J.R., Robb, M.L., Kwong, P.D. and N.A. Doria-Rose, “Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual,” Immunity 50:677-691 (2019) PMID: 30876875 DOI: 10.1016/j.immuni.2019.02.008
Lee, J., Paparoditis, P., Horton, A.P., Frühwirth, A., McDaniel, J.R., Jung, J., Boutz, D.R., Hussein, D.A., Tanno, Y., Pappas, L., Ippolito, G.C., Corti, D., Lanzavecchia, A. and G. Georgiou, “Persistent Antibody Clonotypes Dominate the Serum Response to Influenza over Multiple Years and Repeated Vaccinations,” Cell Host Microbe 25:367-376 (2019) PMID: 30795981 DOI: 10.1016/j.chom.2019.01.010
Manso, T.C., Groenner-Penna, M., Minozzo, J.C., Antunes, B.C., Ippolito, G.C., Molina, F., and Felicori, L.F., “Next-generation sequencing reveals new insights about gene usage and CDR-H3 composition in the horse antibody repertoire,” Mol. Immunology 105:251-259 (2019) PMID: 30562645
2018
Johnson, E.L., Doria-Rose, N.A., Gorman, J., Bhiman, J.N., Schramm, C.A., Vu, A.Q., Law, W.H., Zhang, B., Bekker, V., Karim, S.S.A., Ippolito, G.C., Morris, L., Moore, P.L. Kwong, P.D., Mascola, J.R. and G. Georgiou, “Sequencing HIV-neutralizing antibody exons and introns reveals detailed aspects of lineage maturation” Nature Communications 9(1):4136 (2018) PMID: 30297708 DOI:10.1038/s41467-018-06424-6
Saphire, E.O., Schendel, S.L., Fusco, M.L., Gangavarapu, K., Gunn, B.M., Wec, A.Z., Halfmann, P.J., Brannan, J.M., Herbert, A.S., Qiu, X., Wagh, K., He, S., Giorgi, E.E., Theiler J, Pommert KBJ, Krause TB, Turner HL, Murin CD, Pallesen J, Davidson E, Ahmed R, Aman MJ, Bukreyev A, Burton DR, Crowe JE Jr, Davis CW, Georgiou G, Krammer F, Kyratsous CA, Lai JR, Nykiforuk C, Pauly MH, Rijal P, Takada A, Townsend AR, Volchkov V, Walker LM, Wang CI, Zeitlin L, Doranz BJ, Ward AB, Korber B, Kobinger GP, Andersen KG, Kawaoka Y, Alter G, Chandran K, Dye JM; Viral Hemorrhagic Fever Immunotherapeutic Consortium, “Systematic Analysis of Monoclonal Antibodies against Ebola Virus GP Defines Features that Contribute to Protection,” Cell 2018 Aug 9;174(4):938-952.e13. doi: 10.1016/j.cell.2018.07.033. PMID: 30096313
Triplett, T.A., Garrison, K.C., Marshall, N., Donkor, M., Blazeck, J., Lamb, C., Qerqez A., Dekker, J.D., Tanno, Y. Lu, W-C., Karamitros, S.K., Ford, K., Tan, B., Zhang, M., McGovern, K., Coma, S., Kumada, Y., Yamany, M.S., Sentandreu, S., Fromm, G., Tiziani, S., Schreiber, T.H., Manfredi, M., Ehrlich, L.I.R., Stone E., and G. Georgiou, “Reversal of IDO-Mediated Cancer Immune Suppression by Systemic Kynurenine Depletion with a Therapeutic Enzyme,” Nature Biotechnology doi: 10.1038/nbt.4180
Lee, J. and G. Georgiou, “High Affinity IgA Against Microbial Antigens” Nature Immunology 2018 19:514-515 (2018) PMID: 29777208
Liu, Y., McDaniel, J.R., Khan, S., Campisi, P., Propst, E.J., Holler, T., Grunebaum, E., Georgiou, G., Ippolito, G.C. and Ehrhardt, G.R.A., “Antibodies Encoded by FCRL4-Bearing Memory B Cells Preferentially Recognize Commensal Microbial Antigens,” J Immunology 200:3962-3969 (2018) PMID: 29703863
Chen, Y.Q., Wohlbold, T.J., Zheng, N.Y., Huang, M., Huang, Y., Neu, K.E., Lee, J., Wan, H., Rojas. K.T., Kirkpatrick., E., Henry, C., Palm, A.E., Stamper, C.T., Lan, L.Y., Topham, D.J., Treanor, J., Wrammert, J., Ahmed, R., Eichelberger, M.C., Georgiou, G, Krammer, F. and Wilson, P.C., “Influenza Infection in Humans Induces Broadly Cross-Reactive and Protective Neuraminidase-Reactive Antibodies,” Cell 173:417-429 (2018). PMID: 29625056
- Commentaries and News Stories: Covered by 9 scientific and mainstream media publications
McDaniel, J.R., Pero, S.C., Voss, W.N., Shukla, G.S., Sun, Y., Schaetzle, S., Lee, C.H., Horton, A.P., Harlow, S., Gollihar, J., Ellefson, J.W., Krag, C.C., Tanno, Y., Sidiropoulos, N., Georgiou, G., Ippolito, G.C. and Krag, D.N., “Identification of Tumor-Reactive B Cells and Systemic IgG in Breast Cancer Based on Clonal Frequency in the Sentinel Lymph Node,” Cancer Immunology Immunother 67:729-738 (2018) PMID: 29427082
Wang, B., DeKosky, B.J., Timm, M.R., Lee, J., Normandin, E., Misasi, J., Kong, R., McDaniel, J.R., Delidakis, G., Leigh, K.E., Niezold, T., Choi, C.W., Viox, E.G., Fahad, A., Cagigi, A., Ploquin, A., Leung, K., Yang, E.S., Kong, W.P., Voss, W.N., Schmidt, A.G., Moody, M.A., Ambrozak, D.R., Henry, A.R., Laboune, F., Ledgerwood, J.E., Graham, B.S., Connors, M., Douek, D.C., Sullivan, N.J., Ellington, A.D., Mascola, J.R. and G. Georgiou, “Functional Interrogation and Mining of Natively Paired Human VH:VL Antibody Repertoires,” Nature Biotechnology 36:152-155 (2018) PMID: 29309060
2017
McDaniel, J.R., Ippolito G.C. and G. Georgiou, “Mapping the Secrets of the Antibody Pool,” Nature Biotechnology 35:921-922. (2017) PMID: 28892077.
Jang, S-H., Gregersen, P.K., Georgiou, G., Diamond, B., “Increased Cathepsin S in Dendritic Cells Alters TFH Repertoire and Contributes to Lupus,”, Nature Immunology 18:1016-1024 (2017) PMID: 28692065
Cotham, V.C., Horton, A.P., Lee, J., Georgiou, G. and Brodbelt, J.S., “Middle-down 193-nm Ultraviolet Photodissociation for Unambiguous Antibody Identification and its Implications for Immunoproteomic Analysis,” Anal. Chem. 89:6498-6504 (2017) PMID: 28517930
Lee, C.-H., Romain, G., Yan, W., Watanabe, W., Charab, W., Todorova, B., Lee, Y., Triplett, K., Donkor, M., Lungu, O.I., Lux, A., Marshall, N., Lindorfer, M.A., Richard-Le Goff, O., Balbino, B., Kang, T.H., Tanno, H., Delidakis, G., Alford, C., Taylor, R.P., Nimmerjahn, F., Varadarajan, N., Bruhns, P. Zhang, Y.J. and G. Georgiou, “IgG Fc Domains that Bind C1q but not Effector Fcγ Receptors Delineate the Significance of Complement-Dependent Cell-Mediated Cytotoxicity and Phagocytosis in Antibody Function,” Nature Immunology 18:889-898 (2017) DOI: 10.1038/ni.3770
Lau, D., Lan, L.Y., Andrews, S.F., Henry, C., Thatcher Rojas, K., Neu, K.E., Huang, Y., DeKosky, B., Palm, A.E., Ippolito, G.C., Georgiou, G., Wilson, P., “Low CD21 expression defines a population of recent germinal center graduates primed for plasma cell differentiation” Science Immunology Vol. 2, Issue 7 (2017) DOI: 10.1126/sciimmunol.aai8153
Steiniger, S.C., Glanville, J., Harris, D.W., Wilson, T.L., Ippolito, G.C., and Dunham, S.A., “Comparative analysis of the feline immunoglobulin repertoire,” Biologicals 46:81-87 (2017) PMID: 28131552
Williams, L.D., Ofek, G., Schatzle, S., McDaniel, J.R., Lu, X., Nicely, N.I., Wu, L., Lougheed, C.S., Bradley, T., Louder, M.K., McKee, K., Bailer, R.T., O’Dell, S., Georgiev, I.S., Seaman, M., Parks, R., Marshall, D., Anasti, K., Yang, G., Nie, X., Tumab, N., Wiehe, K., Wagh, K., Korber, B., Kepler, T., Alam, S.M., Morris, L., Kamanga, G., Choen, M.S., Bonsignori, M., Xia, S., Montefiori, D., Kelsoe, G., Gao, F., Mascola, J.R., Moody, M.A., Saunders, K.O., Liao, H., Tomaras, G.D., Georgiou, G., Haynes, B.F., “Potent and broad HIV-neutralizing antibodies in memory B cells and plasma”, Science Immunology 27 Jan 2017: Vol. 2, Issue 7. DOI: 10.1126/sciimmunol.aal2200
Li, Q., Yi, L., Hoi, K.H., Marek, P., Georgiou, G. and Iverson, B.L., “Profiling Protease Specificity: Combining Yeast ER Sequestration Screening (YESS) with Next Generation Sequencing,” ACS Chem. Biol. (2017) PMID: 27977123
Cramer, S.L., Saha, A., Liu, J., Tadi, S., Tiziani, S., Yan, W., Triplett, K., Lamb, C., Alters, S.E., Rowlinson, S., Zhang, Y., Keating, M.J., Huang, P., DiGiovanni, J., Georgiou, G., and E. Stone, “Systemic Depletion of Serum L-Cyst(e)ine with an Engineering Human Enzyme Mediates Potent Induction of ROS Leading to Selective Cancer Ablation,” Nature Medicine 23, 120-127, doi: 10.1038/nm.4232 (2017) PMID: 27869804
- Commentaries and News Stories: Covered by >24 scientific and mainstream media publications; e.g. Cancer Discovery: http://cancerdiscovery.aacrjournals.org/content/early/2016/12/15/2159-8290.CD-RW2016-235 ; Yahoo Finance: http://finance.yahoo.com/news/study-published-nature-medicine-highlights-122000477.html
2016
Wu, G.C., Cheung, N.V., Georgiou , G., Marcotte, E.M., Ippolito, G.C., “Temporal Stability and Molecular Persistence of the Bone Marrow Plasma Cell Antibody Repertoire,” Nature Communications 7:13838 (2016) PMID: 28000661
Lee, J., Boutz, D.R., Chromikova, V., Joyce, M.G., Vollmers, C., Leung, K., Horton, A.P., DeKosky, B.J., Lee, C.H., Lavinder, J.J., Murrin, E.M., Chrysostomou, C., Hoi, K.H., Tsybovsky, Y., Thomas, P.T., Druz, A., Zhang, B., Zhang, Y., Wang, L., Kong, W.P., Park, D., Popova, L.I., Dekker, C.L., Davis, M.M., Bloom, C.E., Ross, T.M., Ellington, A.D., Wilson, P.C., Marcotte, E.M., Mascola, J.R., Ippolito, G.C., Krammer, F., Quake, S.R., Kwong, P.D. and Georgiou, G., “Quantitative, Molecular-Level Analysis of the Serum Antibody Repertoire Reveals Unanticipated Features of the Response to Influenza Vaccine,” Nature Medicine 22:1456-64 (2016) PMID: 27820605
e.g. Drug Discovery & Development: https://www.dddmag.com/news/2016/11/flu-vaccines-effectiveness-could-be-improved-new-findings-suggest
Raymond, D., Stewart, S., Jiwon, L., Ferdman, J., Bajic, G., Khoi, T.D., Ernandes, M.J., Pirada, S., Settembre, Dormitzer, P.R., Del Giudice, G., Finco, O., Kang, T.H., Ippolito, G.C., Georgiou, G., Kepler, T.M., Haynes, B.F., Moody, M.A., Liao, H-X., Schmidt, A.G. and S.C. Harrison, “Influenza Immunization Elicits Hemagglutinin Receptor-Site Antibodies Specific for an Egg-Adapted Vaccine Strain,” Nature Medicine 22:1465-69 (2016) PMID: 27820604
Wang, B., Lee, C.H., Johnson, E.L., Kluwe, C.A., Cunningham, J.C., Tanno, H., Crooks, R.M., Georgiou. G. and A.D. Ellington, “Discovery of High Affinity Anti-Ricin Antibodies by B Cell Receptor Sequencing and by Yeast Display of Combinatorial VH:VL Libraries from Immunized Animals“, MAbs. 25:1-10. (2016) PMID: 27224530
DeKosky, B.J., Lungu, O., Parek, D., Johnson, E.L, Charab, W., Chrysostomou, C., Kuroda, D., Ellington, A.D., Ippolito, G.C., Gray, J.J. and G. Georgiou, “Large-Scale Sequence and Structural Comparisons of Human Naïve and Antigen-Experienced Antibody Repertoires,” Proc. Natl. Acad. Sci. USA 113:E2636-45 (2016) PMID: 27114511
Biswas, P., Chavali, V.R., Agnello, G., Stone, E., Chakarova, C., Duncan, J.L., Kannabiran, C., Homsher, M., Bhattacharya, S.S., Naeem, M.A., Kimchi, A., Sharon, A., Iwata, T., Riazuddin, S. , Reddy, G.B., Hejtmancik, J.F., Georgiou, G., Riazuddin, S.A. and R. Ayyagari, “A Missense Mutation in ASRGL1 is Involved in Causing Autosomal Recessive Retinal Degeneration” Hum Mol Genet. pii: ddw113. (2016) PMID: 27106100
Breski, R.J. and G. Georgiou, “Immunoglobulin Isotype Knowledge and Application to Fc Engineering, “ Curr. Opin. Immunol. 40:62-69 (2016). PMID: 27003675
Dekker, J.D., Park, D., Shaffer, A.L., Kohlhammerc, H., Deng, W., Lee, B.-K., Ippolito, C.C., Georgiou, G., Iyer, V.R., Staudt, L.M., Tucker, H.O., “Subtype Specific Addiction of the Activated B Cell Subset of Diffuse Large B Cell Lymphoma to FOXP1,” Proc. Natl. Acad. Sci. USA 113:E577-86 (2016). PMID: 26844430
McDaniel, J.R., DeKosky, B., Tanno, H., Ellington, A.D. and G. Georgiou, “Ultra- high-throughput sequencing of the immune receptor repertoire from millions of lymphocytes,” Nature Protocols 11:429-42 (2016). PMID: 26787899
Murzabaev, M., Kojima, T., Mizoguchi, T., Kobayashi, I., DeKosky, B.J., Georgiou, G., H. Nakano, “Handmade Microfluidic Device for Biochemical Applications in Emulsion,” J Biosci Bioeng. 121:471-6 (2016) PMID: 26386750
Gorman, J., Soto, C., Yang, M.M., Davenport, T.M, Guttman, M., Bailer, R.T., Chambers, M., Chuang, G.Y., DeKosky, B.J., Doria-Rose, N.A., Druz, A., Ernandes, M.J., Georgiev, I.S., Jarosinski, M.C., Joyce, M.G., Lemmin, T.M., Leung, S., Louder, M.K., McDaniel, J.R., Narpala, S., Pancera, M., Stuckey, J., Wu, X., Yang, Y., Zhang, B., Zhou, T., Program, N.C., Mullikin, J.C., Baxa, U., Georgiou, G., McDermott, A.B., Bonsignori, M., Haynes, B.F., Moore, P.L., Morris, L., Lee, K.K., Shapiro, L., Mascola, J.R., Kwong, P.D., “Structures of HIV-1 Env V1V2 with Broadly Neutralizing Antibodies Reveal Commonalities that Enable Vaccine Design,” Nat Struct. Mol Biol. 23:81-90 (2016). PMID: 26689967
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2015
Quandt, E., Gollihar, J., Blount, Z., Ellington, A.D., Georgiou, G. and J.E Barrick, “Fine-Tuning Citrate Synthase Flux Potentiates and Refines Metabolic Innovation in the Lenski Evolution Experiment,” Elife doi: 10.7554/eLife.09696 (2015) PMID: 26465114.
Burrage, L.C., Sun, Q., Elsea, S.H., Jiang, M.N., Nagamani, S.C., Frankel, A.E., Stone, E., Alters, S.E., Johnson, D.E., Rowlinson, S.W., Georgiou, G, Members of the Urea Cycle Disorders Consortium and B.H. Lee, “Human Recombinant Arginase Enzyme Reduces Plasma Arginine in Mouse Models of Arginase Deficiency,” Hum Mol Genet. 24:6,417-27 (2015). PMID: 26358771
Kerr, S.A., Jackson, E.L., Lungu, O.I., Meyer, A.G., Demogines, A., Ellington, A.D., Georgiou, G., Wilke, C.O. and S. L. Sawyer, “Computational and Functional Analysis of the Virus-Receptor Interface Reveals Host Range Trade-offs in New World Arenaviruses,” J. Virol. 89:11643-53 (2015) PMID: 26355089.
Wang, B., Kluwe, C.A., Lungu, O.I., DeKosky, B.J., Kerr, S.A., Johnson, E.L., Jung, J., Rezigh, A.B., Carroll1, S.M., Reyes, A.N., Bentz, J.R., Villanueva, I., Altman, A.L., Davey, R.A., Ellington, A.D. and G. Georgiou, “Facile Discovery of a Diverse Panel of Anti-Ebola Virus Antibodies by Immune Repertoire Mining,” Scientific Reports 5:13926. doi: 10.1038/srep13926 (2015) PMID: 26355042.
Wine, Y., Horton, A.P., Ippolito, G.C. and Georgiou G., “Serology in the 21st Century: the Molecular-Level Analysis of the Serum Antibody Repertoire,” Curr Opin Immunol. 35:89-97 (2015) PMID: 26172290
Yi, L., Taft, J.M., Li, Q., Gebhard, M.C., Georgiou, G. and Iverson, B.L., “Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast” Methods Mol Biol. 1319:81-93 (2015) PMID: 26060071
Chrysostomou, C., Quandt, E. M., Marshall, N.M., Stone, E. and Georgiou G. “An Alternate Pathway of Arsenate Resistance in E. coli Mediated by the Glutathione-S-Transferase GstB,” ACS Chem Bio (2015) PMID: 25517993
DeKosky, B.J., Kojima, T., Rodin, A., Charab, W., Ippolito, G.C., Ellington, A.D. and G. Georgiou, “Ultra-High Throughput Antibody VH:VL Sequencing Using a Novel Single-Cell Flow Focusing Technology,” Nature Medicine 21:86-91(2015) PMID: 25501908
Lavinder, J.J., Horton, A.P., Georgiou, G and Ippolito G.C., “Next-Generation Sequencing and Protein Mass Spectrometry for the Comprehensive Analysis of Human Cellular and Serum Antibody Repertoires,” Curr Opin Chem Biol. 24:112-120 (2015) PMID: 25461729
2014
Romain G., Senyukov, R.R., Merouane, A., Kelton, W., Liadi, M. A., Georgiou, G., Roysam, B., Lee, D.A. and N. Varadarajan, “Antibody Fc-engineering Improves Frequency and Promotes Kinetic Boosting of Serial Killing Mediated by Natural Killer Cells,” Blood (2014) pii: blood-2014-04-569061. [Epub ahead of print] PMID: 25232058.DeKosky, B.J., Kojima, T., Rodin, A., Charab, W., Ippolito, G.C., Ellington, A.D. and G. Georgiou, “Ultra-High Throughput Antibody VH:VL Sequencing Using a Novel Single-Cell Flow Focusing Technology,” Nature Medicine (accepted)Lavinder, J.J., Hoi, K.H., Reddy, S.T., Wine, Y. and G. Georgiou, “Systematic Characterization and Comparative Analysis of the Rabbit Immunoglobulin Repertoire”, PLoS One 9:e101322 (2014) PMID: 24684310.Boutz, D.R., Horton, A.P., Wine, Y., Lavinder, J., Georgiou, G. and E.M. Marcotte,” “Proteoimic Identification of Monoclonal Antibodies from Serum, Anal. Chem. (2014) PMID: 24684310.Mroczek, E.S., Ippolito, G.C., Rogosch, T., Hoi, K.H., Hwangpo, T.A., Brand, M.G., Zhuang, Y., Liu, C.R., Schneider, D.A., Zemlin, M., Brown, E.E., Georgiou, G., Schroeder, HW Jr. “Differences in the Composition of the Human Antibody Repertoire by B Cell Subsets in the Blood,” Front. Immunol. 19:96 (2014) PMID: 24678310.Laserson, U., Vigneault, F., Gadala-Maria, D., Yaari, G., Uduman, M., Kasvin-Felton, J., Kelton, W., Jung, S.-T., Liu, Y.-P., Lasserson, J., Chari, R., Lee, J., Bachelet, I., Hickey, B., Lieberman-Aiden, E., Hanczaruk, B., Simen, B.B., Egholm, M., Koller, D., Georgiou, G., Kleinstein, S., George Church, “High-Resolution Antibody Dynamics of Vaccine-Induced Immune Responses” Proc. Natl. Acad. Sci. USA 111:4928-4933(2014) PMID: 24639495.Doria-Rose, N.A., Schramm, C.A., Gorman, J., Moore, P.L., Bhiman, J.N., DeKosky, B., Ernandes, M.J., Georgiev, I.S., Kim, H.J., Pancera, M., Staupe, R.P., Altae-Tran, H.R., Bailer, R.T., Crooks, E.T., Cupo, A., Druz, A., Garrett, J.N., Hoi, K.H., Kong, R., Louder, M.K., Longo, N.S., McKee, K., Nonyane, M., O’Dell, S., Roark, R.S., Rudicell, R.S., Schmidt, S.D., Sheward, D.J., Soto, C., Wibmer, C.K., Yang, Y. Zhang, Z., Mullikin, J.C., Binley, J.M., Sanders, R.W., Wilson, I.A., Moore, J.P., Ward, A.B., Georgiou, G., Williamson, C., Abdool Karim, S.S., Morris, L., Kwong, P.D., Shapiro, L., Mascola, J.R., “Developmental Pathway for Potent V1V2-Directed HIV-1-Neutralizing Antibodies,” Nature509:55-62 (2014)PMID: 24590074
Georgiou G., Ippolito, G., Beuasang, G., Busse, C.E., Wardemann, H. and S.R. Quake, “The Promise and Challenge of High-throughput Sequencing of the Antibody Repertoire,” Nature Biotechnol. 32:158-68 (2014). PMID: 24441474.Quandt, E.M., Deatherage, D.E., Ellington, A.D., Georgiou, G. and J. E. Barrick, “Recursive genome-wide recombination and sequencing reveals a key refinement step in the evolution of a metabolic innovation in E. coli,” Proc. Natl. Acad. Sci. USA 111:2217-22 (2014) PMID: 24379390
National Geographic: http://phenomena.nationalgeographic.com/2014/01/06/evolution-hidden-in-plain-sight/; Proc. Natl. Acad. Sci. USA Highlight:.http://www.pnas.org/site/highlights/highlights.xhtml#trait; Proc. Natl. Acad. Sci. USA Commentary: http://www.pnas.org/content/111/6/2056.full; Le Scienze:
http://www.lescienze.it/news/2014/01/03/news/nuovo_tratto_successo_evolutivo_raffinamento-1946457/; Technology.org: http://www.technology.org/2014/01/29/unpacking-past-identifying-key-evolutionary-step-e-coli-metabolism/
Lavinder, J., Wine, Y., Giesecke, C., Ippolito, G.C., Horton, A.P., Hoi, K.-H., DeKosky, B.J., Ellington, A.D., Dörner, T., Marcotte, E.M., Boutz, D.R. and G. Georgiou, “Dynamics, Functionality and Discordance of the Human Serum Antibody and BCR Repertoires Following Immunization” Proc. Natl. Acad. Sci. USA 111:2259-64(2014) PMID: 24469811
2013
Tanios, R., Bekdash, A., Kassab. E., Stone, E., Georgiou, G., Frankel, A.E., Abi-Habib, R.J. “Human Recombinant Arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-Induced Arginine Depletion is Selectively Cytotoxic to Human Acute Myeloid Leukemia Cells,” Leuk Res. 37:1565-71 (2013) PMID: 24018014.Paley, O., Agnello, G., Cantor, J., Yoo, T.H., Georgiou, G., Stone E.M., “GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria,” Methods Mol. Biol. 978:31-44 (2013) PMID: 23423887DeKosky, B.J., Ippolito, G.C., Deschner, R.P., Lavinder, J.J., Rawlings, M.P., Varadarajan, N., Wine, Y., Giesecke, C., Dörner, T., Andrews, S.F., Wilson, P.C., Hunicke-Smith, S.P., Wilson, C.P., Ellington, A.D. and G. Georgiou, “High Throughput Determination of the Antibody VH:VL Paired Repertoires,” Nature Biotechnol. 31:166-69(2013) PMID: 23334449Needham, B.D., Carroll, S., Giles, D.K., Georgiou, G., Whiteley, M., and Trent, M.S. “Modulating the Innate Immune Response by Combinatorial Engineering of Endotoxin” Proc. Natl. Acad. Sci. USA 110:1464-69 (2013) PMID: 23297218
http://www.the-scientist.com//?articles.view/articleNo/34313/title/Bacteria-Boost-Vaccine-Effectiveness/
Wine, Y., Boutz, D., Lavinder, J.J., Horton, A., Miklos, A.E., Hughes, R.A., Jung, S.T, Murrin E.M., Ellington, A.D., Marcotte, E.M. and G. Georgiou, “Molecular Deconvolution of the Monoclonal Antibodies that Comprise the Polyclonal Serum Response,” Proc. Natl. Acad. Sci. USA 110: 2993-8(2013) PMID: 23382245Carroll, S. and G. Georgiou,“Antibody-Mediated Inhibition of Human C1s and the Classical Complement Pathway, “Immunobiology doi:pii: S0171-2985(13)00006-5. 10.1016/j.imbio.2013.01.001 (2013).Mittal, J., Yoo, T-H., Georgiou, G. and T.M. Truskett, “Structural Ensemble of an Intrinsically Disordered Polypeptide” J Phys Chem B. 117:118-24 (2013) PMID: 23205890Jung, S. T., Kelton, W., Kang, T. H.; Ng, D., Anderssen, J. T.; Sandlie, I., Sarkar, C., G. Georgiou,
“Effective Phagocytosis of Low Her2 Tumor Cell Lines with Engineered, Aglycosylated IgG Displaying High FcγRIIa Affinity and Selectivity” ACS Chem. Bio. 8:368-75 (2013) PMID: 23030766
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2012
Saggy, I., Wine, Y., Georgiou G. and Benhar, I., “Antibody Isolation from Immunized Animals: Comparison of Phage Display and Antibody Discovery via V gene Repertoire Mining” PEDS (2012) PMID: 22988130Stone, E.M., Paley, O., Hu, J., Ekerdt, B.; Cheung, N.-K. and G., George, “De novo Engineering of a Human Methionine-γ-Lyase for Systemic L-Met Depletion Cancer Therapy,” ACS Chem. Bio. (2012) PMID: 22963240
Romero, P.A., Stone, E.M., Lamb, C., Chantranupong, L., Krause, A., Miklos, A.E., Hughes, R.A., Fechtel, B., Ellington, D.A., Arnold, F.H. and G. Georgiou, “SCHEMA-Designed Variants of Human Arginase I and II Reveal Sequence Elements Important to Stability and Catalysis”, ACS Synth. Biol. 1:221-28 (2012) PMID: 22737599Li W., Cantor, J., Yogesha, S.D., Yang, S., Chantranupong, L., Liu, J.Q., Agnello, G., G. Georgiou, Stone E.M., and Y. Zhang,“Uncoupling Intramolecular Processing and Substrate Hydrolysis in the N-terminal Hydrolase hASRGL1 by Circular Permutation” ACS Chem. Bio. Epub ahead of print (2012) PMID: 22891768Borrok, J.M., Jung, S.T., Kang, T.H. ; Monzingo, A. and G. Georgiou, Revisiting the Role of Glycosylation in the Structure of Human IgG Fc” ACS Chem. Bio (2012) PMID: 22747430Rani, M., Bolles, M., Donaldson, E.F., Van Blarcom, T., Baric, R., Iverson, B.L. and G. Georgiou “Increased Antibody Affinity Confers Broad In Vitro Protection Against Escape Mutants of SARS-CoV” J. Virology 86:9113-21 (2012) PMID: 2269665Skretas, G. Makino, T. Varadarajan N., Pogson M. and G. Georgiou, “Multi-Copy Genes that Enhance the Yield of Mammalian G Protein-Coupled Receptors in Escherichia coli” Metabolic Eng. 14:591-602 (2012) PMID: 22609824Mauldin, J.P., Zeinali, I., Kleypas, K., Woo, J.H., Blackwood, R.S., Jo, C.H., Stone, E.M., Georgiou G., Frankel A. E.,Transl. Oncology 5:26-31 (2012) PMID: 22348173Ippolito, G.C., Hoi K.H., Reddy S.T., Carroll S.M., Ge X., Rogosch T., Zemlin M., Shultz L.D., Ellington A.D., Vandenberg C.L., and G. Georgiou, “Antibody Repertoires in Humanized NOD-scid-IL2Rγnull Mice
and Human B Cells Reveals Human-like Diversification and Tolerance Checkpoints in the Mouse” PLoS One7:e35497 (2012) PMID: 2255816Miklos, A.E., Kluwe, C., Der. B.S., Pai, S., Sircar, A., Hughes, R.A., Berrondo, M., Xu, J., Codrea, V., Buckley,
P.E., Calm, A.M., Walsh, H.S., Warner, C.R., Zacharko, M.A., Carney, J.P., Georgiou, G., Kuhlman, B. and A.D. Ellington, “Structure-Based Design of Supercharged, Highly Thermostable Antibodies,” Chem. & Biol. 19:449-55 (2012) PMID: 22520751Yoo, T-H., Pogson, M., Iverson, B.L. and G. Georgiou, “Directed Evolution of Highly Selective Proteases Using a Novel FACS Based Screen that Capitalizes on the p53 Regulator MDM2” ChemBiochem 3:649-53(2012) PMID: 22334509Cantor, J.R., Panayotou, V., Agnello, G., Stone. E.M. and G. Georgiou, “Engineering Reduced Immuno–genicity Enzymes for Amino Acid Depletion Therapy in Cancer,” Methods Enzymol. 502:291-312 (2012). PMID:22208990Agrawal, V., Boyer, A.L., Woo, J-H., Mauldin,J.P., Chanhee, J. Stone, E.M., Georgiou, G. and Arthur E. Frankel, ” Cytotoxicity of Human Recombinant Arginase I [Co] PEG5000 in the Presence of L-Citruline is Dependent on Arginosuccinate Synthetase Expression in Human Cells”Anti-Cancer Drugs 23:51-64 (2012)PMID:21955999Stone, E. M., Chantrranupong L., Gonzalez, C., O’Neal, J., Rani, M., VanDenBerg, C. and G. Georgiou, “Strategies for Optimizing the Serum Persistence of Engineered Human Arginase I for Cancer Therapy,” J. Control. Release58L171-9 (2012) PMID 22001609.
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