Publications

2017

Lee, C.-H., Romain, G., Yan, W., Watanabe, W., Charab, W., Todorova, B., Lee, Y., Triplett, K., Donkor, M., Lungu, O.I., Lux, A., Marshall, N., Lindorfer, M.A., Richard-Le Goff, O., Balbino, B., Kang, T.H., Tanno, H., Delidakis, G., Alford, C., Taylor, R.P., Nimmerjahn, F., Varadarajan, N., Bruhns, P. Zhang, Y.J.  and G.Georgiou, “IgG Fc Domains that Bind C1q but not Effector Fcγ Receptors Delineate the Significance of Complement-Dependent Cell-Mediated Cytotoxicity and Phagocytosis in Antibody Function”  Nature Immunology (accepted, 2017)

Lau, D., Lan, L.Y., Andrews, S.F., Henry, C., Thatcher Rojas, K., Neu, K.E., Huang, Y., DeKosky, B., Palm, A.E., Ippolito, G.C., Georgiou, G., Wilson, P., “Low CD21 expression defines a population of recent germinal center graduates primed for plasma cell differentiation” Science Immunology Vol. 2, Issue 7 (2017) DOI: 10.1126/sciimmunol.aai8153

Williams, L.D., Ofek, G., Schatzle, S., McDaniel, J.R., Lu, X., Nicely, N.I., Wu, L., Lougheed, C.S., Bradley, T., Louder, M.K., McKee, K., Bailer, R.T., O’Dell, S., Georgiev, I.S., Seaman, M., Parks, R., Marshall, D., Anasti, K., Yang, G., Nie, X., Tumab, N., Wiehe, K., Wagh, K., Korber, B., Kepler, T., Alam, S.M., Morris, L., Kamanga, G., Choen, M.S., Bonsignori, M., Xia, S., Montefiori, D., Kelsoe, G., Gao, F., Mascola, J.R., Moody, M.A., Saunders, K.O., Liao, H., Tomaras, G.D., Georgiou, G., Haynes, B.F., “Potent and broad HIV-neutralizing antibodies in memory B cells and plasma”, Science Immunology 27 Jan 2017: Vol. 2, Issue 7. DOI: 10.1126/sciimmunol.aal2200

Li, Q., Yi, L., Hoi, K.H., Marek, P., Georgiou, G. and Iverson, B.L., “Profiling Protease Specificity: Combining Yeast ER Sequestration Screening (YESS) with Next Generation Sequencing,” ACS Chem. Biol. Doi:10.1021/acschembio.6b00547 [Epub ahead of print] (2017) PMID: 27977123

Cramer, S.L., Saha, A., Liu, J., Tadi, S., Tiziani, S., Yan, W., Triplett, K., Lamb, C., Alters, S.E., Rowlinson, S., Zhang, Y., Keating, M.J., Huang, P., DiGiovanni, J., Georgiou, G., and E. Stone, “Systemic Depletion of Serum L-Cyst(e)ine with an Engineering Human Enzyme Mediates Potent Induction of ROS Leading to Selective Cancer Ablation,” Nature Medicine 23, 120-127, doi: 10.1038/nm.4232 (2017) PMID: 27869804

  • Commentaries and News Stories: Covered by >24 scientific and mainstream media publications;
    e.g. Cancer Discovery: http://cancerdiscovery.aacrjournals.org/content/early/2016/12/15/2159-8290.CD-RW2016-235 ; Yahoo Finance: http://finance.yahoo.com/news/study-published-nature-medicine-highlights-122000477.html

2016

Wu, G.C., Cheung, N.V., Georgiou , G., Marcotte, E.M., Ippolito, G.C., “Temporal Stability and Molecular Persistence of the Bone Marrow Plasma Cell Antibody Repertoire,” Nat Commun. 7:13838 doi: 10.1038/ncomms13838 (2016) PMID: 28000661

Lee, J., Boutz, D.R., Chromikova, V., Joyce, M.G., Vollmers, C., Leung, K., Horton, A.P., DeKosky, B.J., Lee, C.H., Lavinder, J.J., Murrin, E.M., Chrysostomou, C., Hoi, K.H., Tsybovsky, Y., Thomas, P.T., Druz, A., Zhang, B., Zhang, Y., Wang, L., Kong, W.P., Park, D., Popova, L.I., Dekker, C.L., Davis, M.M., Bloom, C.E., Ross, T.M., Ellington, A.D., Wilson, P.C., Marcotte, E.M., Mascola, J.R., Ippolito, G.C., Krammer, F., Quake, S.R., Kwong, P.D. and Georgiou, G., “Quantitative, Molecular-Level Analysis of the Serum Antibody Repertoire Reveals Unanticipated Features of the Response to Influenza Vaccine,” Nature Medicine 22:1456-64 (2016) PMID: 27820605

  • Commentaries and News Stories: Covered by >20 scientific and mainstream media publications;
    e.g. Drug Discovery & Development: https://www.dddmag.com/news/2016/11/flu-vaccines-effectiveness-could-be-improved-new-findings-suggest

Raymond, D., Stewart, S., Jiwon, L., Ferdman, J., Bajic, G., Khoi, T.D., Ernandes, M.J., Pirada, S., Settembre, Dormitzer, P.R., Del Giudice, G., Finco, O., Kang, T.H., Ippolito, G.C., Georgiou, G., Kepler, T.M., Haynes, B.F., Moody, M.A., Liao, H-X., Schmidt, A.G. and S.C. Harrison, “Influenza Immunization Elicits Hemagglutinin Receptor-Site Antibodies Specific for an Egg-Adapted Vaccine Strain,” Nature Medicine 22:1465-69 (2016) PMID: 27820604

Wang, B., Lee, C.H., Johnson, E.L., Kluwe, C.A., Cunningham, J.C., Tanno, H., Crooks, R.M., Georgiou. G. and A.D. Ellington,  “Discovery of High Affinity Anti-Ricin Antibodies by B Cell Receptor Sequencing and by Yeast Display of Combinatorial VH:VL Libraries from Immunized Animals“, MAbs. 25:1-10. (2016) PMID: 27224530

DeKosky, B.J., Lungu, O., Parek, D., Johnson, E.L, Charab, W.,  Chrysostomou, C., Kuroda, D., Ellington, A.D., Ippolito, G.C., Gray, J.J. and G. Georgiou, “Large-Scale Sequence and Structural Comparisons of Human Naïve and Antigen-Experienced Antibody Repertoires,” Proc. Natl. Acad. Sci. USA 113:E2636-45 (2016) PMID: 27114511.

Biswas, P., Chavali, V.R., Agnello, G., Stone, E., Chakarova, C., Duncan, J.L., Kannabiran, C., Homsher, M., Bhattacharya, S.S., Naeem, M.A., Kimchi, A., Sharon, A., Iwata, T., Riazuddin, S. , Reddy, G.B., Hejtmancik, J.F., Georgiou, G., Riazuddin, S.A. and R. Ayyagari, “A Missense Mutation in ASRGL1 is Involved in Causing Autosomal Recessive Retinal Degeneration” Hum Mol Genet. pii: ddw113. (2016) PMID: 27106100

Brexski, R.J. and G. Georgiou, “Immunoglobulin Isotype Knowledge and Application to Fc Engineering, “ Curr. Opin. Immunol. 40:62-69 (2016). PMID: 27003675

Dekker, J.D., Park, D., Shaffer, A.L., Kohlhammerc, H., Deng, W., Lee, B.-K., Ippolito, C.C., Georgiou, G., Iyer, V.R., Staudt, L.M., Tucker, H.O., “Subtype Specific Addiction of the Activated B Cell Subset of Diffuse Large B Cell Lymphoma to FOXP1,” Proc. Natl. Acad. Sci. USA 113:E577-86 (2016). PMID: 26844430

McDaniel, J.R., DeKosky, B., Tanno, H., Ellington, A.D. and G. Georgiou, “Ultra- high-throughput sequencing of the immune receptor repertoire from millions of lymphocytes,” Nature Protocols 11:429-42 (2016). PMID: 26787899

Murzabaev, M., Kojima, T., Mizoguchi, T., Kobayashi, I., DeKosky, B.J., Georgiou, G., H. Nakano, “Handmade Microfluidic Device for Biochemical Applications in Emulsion,” J Biosci Bioeng. 121:471-6 (2016) PMID: 26386750

Gorman, J., Soto, C., Yang, M.M., Davenport, T.M, Guttman, M., Bailer, R.T., Chambers, M., Chuang, G.Y., DeKosky, B.J., Doria-Rose, N.A., Druz, A., Ernandes, M.J., Georgiev, I.S., Jarosinski, M.C., Joyce, M.G., Lemmin, T.M., Leung, S., Louder, M.K., McDaniel, J.R., Narpala, S., Pancera, M., Stuckey, J., Wu, X., Yang, Y., Zhang, B., Zhou, T., Program, N.C., Mullikin, J.C., Baxa, U., Georgiou, G., McDermott, A.B., Bonsignori, M., Haynes, B.F., Moore, P.L., Morris, L., Lee, K.K., Shapiro, L., Mascola, J.R., Kwong, P.D., “Structures of HIV-1 Env V1V2 with Broadly Neutralizing Antibodies Reveal Commonalities that Enable Vaccine Design,” Nat Struct. Mol Biol. 23:81-90 (2016). PMID: 26689967

2015

Quandt, E., Gollihar, J., Blount, Z., Ellington, A.D., Georgiou, G. and J.E Barrick, “Fine-Tuning Citrate Synthase Flux Potentiates and Refines Metabolic Innovation in the Lenski Evolution Experiment,” Elife doi: 10.7554/eLife.09696 (2015) PMID: 26465114.

Burrage, L.C., Sun, Q., Elsea, S.H., Jiang, M.N., Nagamani, S.C., Frankel, A.E., Stone, E., Alters, S.E., Johnson, D.E., Rowlinson, S.W., Georgiou, G, Members of the Urea Cycle Disorders Consortium and B.H. Lee, “Human Recombinant Arginase Enzyme Reduces Plasma Arginine in Mouse Models of Arginase Deficiency,” Hum Mol Genet. 24:6,417-27 (2015). PMID: 26358771

Kerr, S.A., Jackson, E.L., Lungu, O.I., Meyer, A.G., Demogines, A., Ellington, A.D., Georgiou, G., Wilke, C.O. and S. L. Sawyer, “Computational and Functional Analysis of the Virus-Receptor Interface Reveals Host Range Trade-offs in New World Arenaviruses,” J. Virol. 89:11643-53 (2015) PMID: 26355089.

Wang, B., Kluwe, C.A., Lungu, O.I., DeKosky, B.J., Kerr, S.A., Johnson, E.L., Jung, J., Rezigh, A.B., Carroll1, S.M., Reyes, A.N., Bentz, J.R., Villanueva, I., Altman, A.L., Davey, R.A., Ellington, A.D. and G. Georgiou, “Facile Discovery of a Diverse Panel of Anti-Ebola Virus Antibodies by Immune Repertoire Mining,” Scientific Reports 5:13926. doi: 10.1038/srep13926 (2015) PMID: 26355042.

Wine, Y., Horton, A.P., Ippolito, G.C. and Georgiou G., “Serology in the 21st Century: the Molecular-Level Analysis of the Serum Antibody Repertoire,” Curr Opin Immunol. 35:89-97 (2015) PMID: 26172290

Yi, L., Taft, J.M., Li, Q., Gebhard, M.C., Georgiou, G. and Iverson, B.L., “Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast” Methods Mol Biol. 1319:81-93 (2015) PMID: 26060071

Chrysostomou, C., Quandt, E. M., Marshall, N.M., Stone, E. and Georgiou G.  “An Alternate Pathway of Arsenate Resistance in E. coli Mediated by the Glutathione-S-Transferase GstB,” ACS Chem Bio (2015) PMID: 25517993

DeKosky, B.J., Kojima, T., Rodin, A., Charab, W., Ippolito, G.C., Ellington, A.D. and G. Georgiou, “Ultra-High Throughput Antibody VH:VL Sequencing Using a Novel Single-Cell Flow Focusing Technology,” Nature Medicine 21:86-91(2015) PMID: 25501908

Lavinder, J.J., Horton, A.P., Georgiou, G and Ippolito G.C., “Next-Generation Sequencing and Protein Mass Spectrometry for the Comprehensive Analysis of Human Cellular and Serum Antibody Repertoires,” Curr Opin Chem Biol.  24:112-120 (2015) PMID: 25461729

2014

Kelton, W., Mehta, N., Charab, W., Lee, J., Kojima, T., Kang, T.-H. and G. Georgiou, “IgGA: A “Cross-Isotype” Engineered Human Fc Antibody Domain that Displays Both IgG-like and IgA-like Effector Functions,”  Chem & Biol. 21:1603-9 (2014) PMID: 25500223

  • Chem & Bio featured Article

Romain G., Senyukov, R.R., Merouane, A., Kelton, W., Liadi, M. A., Georgiou, G., Roysam, B., Lee, D.A. and N. Varadarajan, “Antibody Fc-engineering Improves Frequency and Promotes Kinetic Boosting of Serial Killing Mediated by Natural Killer Cells,” Blood (2014) pii: blood-2014-04-569061. [Epub ahead of print] PMID: 25232058.

DeKosky, B.J., Kojima, T., Rodin, A., Charab, W., Ippolito, G.C., Ellington, A.D. and G. Georgiou, “Ultra-High Throughput Antibody VH:VL Sequencing Using a Novel Single-Cell Flow Focusing Technology,” Nature Medicine (accepted)

Lavinder, J.J., Hoi, K.H., Reddy, S.T., Wine, Y. and G. Georgiou, “Systematic Characterization and Comparative Analysis of the Rabbit Immunoglobulin Repertoire”, PLoS One 9:e101322 (2014) PMID: 24684310.

Boutz, D.R., Horton, A.P., Wine, Y., Lavinder, J., Georgiou, G. and E.M. Marcotte,” “Proteoimic Identification of Monoclonal Antibodies from Serum, Anal. Chem. (2014) PMID: 24684310.

Mroczek, E.S., Ippolito, G.C., Rogosch, T., Hoi, K.H., Hwangpo, T.A., Brand, M.G., Zhuang, Y., Liu, C.R., Schneider, D.A., Zemlin, M., Brown, E.E., Georgiou, G., Schroeder, HW Jr. “Differences in the Composition of the Human Antibody Repertoire by B Cell Subsets in the Blood,” Front.  Immunol. 19:96 (2014) PMID: 24678310.

Laserson, U., Vigneault, F., Gadala-Maria, D., Yaari, G., Uduman, M., Kasvin-Felton, J., Kelton, W., Jung, S.-T., Liu, Y.-P., Lasserson, J., Chari, R., Lee, J., Bachelet, I., Hickey, B., Lieberman-Aiden, E., Hanczaruk, B., Simen, B.B., Egholm, M., Koller, D., Georgiou, G., Kleinstein, S., George Church,  “High-Resolution Antibody Dynamics of Vaccine-Induced Immune Responses” Proc. Natl. Acad. Sci. USA 111:4928-4933(2014) PMID: 24639495.

Doria-Rose, N.A., Schramm, C.A., Gorman, J., Moore, P.L., Bhiman, J.N., DeKosky, B., Ernandes, M.J., Georgiev, I.S., Kim, H.J., Pancera, M., Staupe, R.P., Altae-Tran, H.R., Bailer, R.T., Crooks, E.T., Cupo, A., Druz, A., Garrett, J.N., Hoi, K.H., Kong, R., Louder, M.K., Longo, N.S., McKee, K., Nonyane, M., O’Dell, S., Roark, R.S., Rudicell, R.S., Schmidt, S.D., Sheward, D.J., Soto, C., Wibmer, C.K., Yang, Y. Zhang, Z., Mullikin, J.C., Binley, J.M., Sanders, R.W., Wilson, I.A., Moore, J.P., Ward, A.B., Georgiou, G., Williamson, C., Abdool Karim, S.S., Morris, L., Kwong, P.D., Shapiro, L., Mascola, J.R., “Developmental Pathway for Potent V1V2-Directed HIV-1-Neutralizing Antibodies,”  Nature509:55-62 (2014)PMID: 24590074

Georgiou G., Ippolito, G., Beuasang, G., Busse, C.E., Wardemann, H. and S.R. Quake, “The Promise and Challenge of High-throughput Sequencing of the Antibody Repertoire,” Nature Biotechnol. 32:158-68 (2014).  PMID: 24441474.

Quandt, E.M., Deatherage, D.E., Ellington, A.D., Georgiou, G. and J. E. Barrick, “Recursive genome-wide recombination and sequencing reveals a key refinement step in the evolution of a metabolic innovation in E. coli,”  Proc. Natl. Acad. Sci. USA 111:2217-22 (2014) PMID: 24379390

Lavinder, J., Wine, Y., Giesecke, C., Ippolito, G.C., Horton, A.P., Hoi, K.-H., DeKosky, B.J., Ellington, A.D., Dörner, T., Marcotte, E.M., Boutz, D.R. and G. Georgiou,  “Dynamics, Functionality and Discordance of the Human Serum Antibody and BCR Repertoires Following Immunization” Proc. Natl. Acad. Sci. USA 111:2259-64(2014) PMID: 24469811

2013

Tanios, R., Bekdash, A., Kassab. E., Stone, E., Georgiou, G., Frankel, A.E., Abi-Habib, R.J. “Human Recombinant Arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-Induced Arginine Depletion is Selectively Cytotoxic to Human Acute Myeloid Leukemia Cells,” Leuk Res. 37:1565-71 (2013) PMID: 24018014.

Paley, O., Agnello, G., Cantor, J., Yoo, T.H., Georgiou, G., Stone E.M., “GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria,” Methods Mol. Biol. 978:31-44 (2013) PMID: 23423887

DeKosky, B.J., Ippolito, G.C., Deschner, R.P., Lavinder, J.J., Rawlings, M.P., Varadarajan, N., Wine, Y., Giesecke, C., Dörner, T., Andrews, S.F., Wilson, P.C., Hunicke-Smith, S.P., Wilson, C.P., Ellington, A.D. and G. Georgiou, “High Throughput Determination of the Antibody VH:VL Paired Repertoires,” Nature Biotechnol. 31:166-69(2013) PMID: 23334449

Needham, B.D., Carroll, S., Giles, D.K., Georgiou, G., Whiteley, M., and Trent, M.S.  “Modulating the Innate Immune Response by Combinatorial Engineering of Endotoxin” Proc. Natl. Acad. Sci. USA 110:1464-69 (2013) PMID: 23297218

Wine, Y., Boutz, D., Lavinder, J.J., Horton, A., Miklos, A.E., Hughes, R.A., Jung, S.T, Murrin E.M., Ellington, A.D., Marcotte, E.M. and G. Georgiou,  “Molecular Deconvolution of the Monoclonal Antibodies that Comprise the Polyclonal Serum Response,” Proc. Natl. Acad. Sci. USA 110: 2993-8(2013) PMID: 23382245

Carroll, S. and G. Georgiou,“Antibody-Mediated Inhibition of Human C1s and the Classical Complement Pathway, “Immunobiology doi:pii: S0171-2985(13)00006-5. 10.1016/j.imbio.2013.01.001 (2013).

Mittal, J., Yoo, T-H., Georgiou, G. and T.M. Truskett, “Structural Ensemble of an Intrinsically Disordered Polypeptide” J Phys Chem B. 117:118-24 (2013) PMID: 23205890

Jung, S. T., Kelton, W., Kang, T. H.; Ng, D., Anderssen, J. T.; Sandlie, I., Sarkar, C., G. Georgiou,
“Effective Phagocytosis of Low Her2 Tumor Cell Lines with Engineered, Aglycosylated IgG Displaying High FcγRIIa Affinity and Selectivity” ACS Chem. Bio. 8:368-75 (2013) PMID: 23030766

2012

Saggy, I., Wine, Y., Georgiou G. and Benhar, I., “Antibody Isolation from Immunized Animals: Comparison of Phage Display and Antibody Discovery via V gene Repertoire Mining” PEDS (2012) PMID: 22988130

Stone, E.M., Paley, O., Hu, J., Ekerdt, B.; Cheung, N.-K. and G., George, “De novo Engineering of a Human Methionine-γ-Lyase for Systemic L-Met Depletion Cancer Therapy,” ACS Chem. Bio. (2012) PMID: 22963240

Romero, P.A., Stone, E.M., Lamb, C., Chantranupong, L., Krause, A., Miklos, A.E., Hughes, R.A., Fechtel, B., Ellington, D.A., Arnold, F.H. and G. Georgiou, “SCHEMA-Designed Variants of Human Arginase I and II Reveal Sequence Elements Important to Stability and Catalysis”, ACS Synth. Biol. 1:221-28 (2012) PMID: 22737599

Li W., Cantor, J., Yogesha, S.D.,  Yang, S., Chantranupong, L., Liu, J.Q., Agnello, G., G. Georgiou, Stone E.M.,  and Y. Zhang,“Uncoupling Intramolecular Processing and Substrate Hydrolysis in the N-terminal Hydrolase hASRGL1 by Circular Permutation” ACS Chem. Bio. Epub ahead of print (2012) PMID: 22891768

Borrok, J.M., Jung, S.T., Kang, T.H. ; Monzingo, A. and G. Georgiou, Revisiting the Role of Glycosylation in the Structure of Human IgG Fc”  ACS Chem. Bio (2012) PMID: 22747430

Rani, M., Bolles, M., Donaldson, E.F., Van Blarcom, T.,  Baric, R., Iverson, B.L. and G. Georgiou “Increased Antibody Affinity Confers Broad In Vitro Protection Against Escape Mutants of SARS-CoV”  J. Virology 86:9113-21 (2012)  PMID: 2269665

Skretas, G.  Makino, T. Varadarajan N.,  Pogson M. and G. Georgiou,  “Multi-Copy Genes that Enhance the Yield of Mammalian G Protein-Coupled Receptors in Escherichia coliMetabolic Eng. 14:591-602 (2012) PMID: 22609824

Mauldin, J.P., Zeinali, I., Kleypas, K., Woo, J.H., Blackwood, R.S., Jo, C.H., Stone, E.M., Georgiou G., Frankel A. E.,Transl. Oncology 5:26-31 (2012) PMID: 22348173

Ippolito, G.C.,  Hoi K.H., Reddy S.T., Carroll S.M., Ge X., Rogosch T., Zemlin M., Shultz L.D., Ellington A.D., Vandenberg C.L., and G. Georgiou, “Antibody Repertoires in Humanized NOD-scid-IL2Rγnull Mice
and Human B Cells Reveals Human-like Diversification and Tolerance Checkpoints in the Mouse” PLoS One7:e35497 (2012) PMID: 2255816

Miklos, A.E., Kluwe, C., Der. B.S., Pai, S., Sircar, A., Hughes, R.A., Berrondo, M., Xu, J., Codrea, V., Buckley,
P.E., Calm, A.M., Walsh, H.S., Warner, C.R., Zacharko, M.A., Carney, J.P., Georgiou, G., Kuhlman, B. and A.D. Ellington,  “Structure-Based Design of Supercharged, Highly Thermostable Antibodies,”  Chem. & Biol.  19:449-55 (2012) PMID: 22520751

Yoo, T-H., Pogson, M., Iverson, B.L. and G. Georgiou,  “Directed Evolution of Highly Selective Proteases Using a Novel FACS Based Screen that Capitalizes on the p53 Regulator MDM2” ChemBiochem 3:649-53(2012) PMID: 22334509

Cantor,  J.R., Panayotou, V., Agnello, G., Stone. E.M. and G. Georgiou, “Engineering Reduced Immuno–genicity Enzymes for Amino Acid Depletion Therapy in Cancer,”  Methods Enzymol. 502:291-312 (2012). PMID:22208990

Agrawal, V., Boyer, A.L., Woo, J-H., Mauldin,J.P., Chanhee, J. Stone, E.M., Georgiou, G. and Arthur E. Frankel, ” Cytotoxicity of Human Recombinant Arginase I [Co] PEG5000 in the Presence of L-Citruline is Dependent on Arginosuccinate Synthetase Expression in Human Cells”Anti-Cancer Drugs 23:51-64 (2012)PMID:21955999

Stone, E. M., Chantrranupong L., Gonzalez, C., O’Neal, J., Rani, M., VanDenBerg, C. and G. Georgiou, “Strategies for Optimizing the Serum Persistence of Engineered Human Arginase I for Cancer Therapy,” J. Control. Release58L171-9 (2012) PMID 22001609.