Taft, J.M., Georgeon, S., Allen, C., Reckel, S., DeSautelle, J., Hantschel, O., Georgiou, G., Iverson, B.L., “Rapid Screen for Tyrosine Kinase Inhibitor Resistance Mutations and Substrate Specificity,” ACS Chem Biol. 14(9):1888-1895 (2019) PMID: 31339688, DOI: 10.1021/acschembio.9b00283.

Kshattry, S., Saha, A., Gries, P., Tiziani, S., Stone, EM., Georgiou, G., DiGiovanni, J., “Enzyme-mediated depletion of l-cyst(e)ine synergizes with thioredoxin reductase inhibition for suppression of pancreatic tumor growth,” NPJ Precis Oncol. (2019) eCollection 2019, PMID: 31231686. DOI: 10.1038/s41698-019-0088-z.

Lindesmith, L.C., McDaniel, J.R., Changela, A., Varardi, R., Kerr S.A., Constantini, V., Brewer-Jensen, P.D., Mallory, M.L., Voss, W.N., Boutz, D.R., Blazeck, J. J., Ippolito, G.C., Vinje, J., Kwong, P.D., Georgiou, G. and Baric, R. “Boosted Broad Binding/Blockade Antibodies Dominate the Serological Repertoire to Norovirus Vaccine” Immunity 50: 1530-1541(2019) PMID: 31216462 DOI: 10.1016/j.immuni.2019.05.007

Wang, W., Green, M., Choi, J.E., Gijón, M., Kennedy, P.D., Johnson, J.K., Liao, P., Lang, X., Kryczek, I., Sell, A., Xia, H., Zhou, J., Li, G., Li, J., Li, W., Wei, S., Vatan, L., Zhang, H., Szeliga, W., Gu, W., Liu, R., Lawrence, T.S., Lamb, C., Tanno, Y., Cieslik, M., Stone, E., Georgiou. G., Chan, T.A., Chinnaiyan, A. and W. Zou, “CD8+ T Cells Regulate Tumour Ferroptosis During Cancer Immunotherapy” Nature (2019) PMID: 31043744 DOI: 10.1038/s41586-019-1170-y

Kang, T.H., Lee, C.H., Delidakis, G., Jung, J., Richard-Le Goff, O., Lee, J., Kim, J.E., Charab, W., Bruhns, P. and G. Georgiou, “An Engineered Human Fc variant With Exquisite Selectivity for FcγRIIIaV158 Reveals That Ligation of FcγRIIIa Mediates Potent Antibody Dependent Cellular Phagocytosis With GM-CSF-Differentiated Macrophages,” Front Immunology 10:562 (2019) PMID: 30984171 DOI: 10.3389/fimmu.2019.00562

Krebs, S.J., Kwon, Y.D., Schramm, C.A., Law, W.H., Donofrio, G., Zhou, K.H., Gift, S., Dussupt, V., Georgiev, I.S., Schätzle, S., McDaniel, J.R., Lai, Y.T., Sastry, M., Zhang, B., Jarosinski, M.C., Ransier, A., Chenine, A.L., Asokan, M., Bailer, R.T., Bose, M., Cagigi, A., Cale, E.M., Chuang, G.Y., Darko, S., Driscoll, J.I., Druz, A., Gorman, J., Laboune, F., Louder, M.K., McKee, K., Mendez, L., Moody, M.A., O’Sullivan, A.M., Owen, C., Peng, D., Rawi, R., Sanders-Buell, E., Shen, C.H., Shiakolas, A.R., Stephens, T., Tsybovsky, Y., Tucker, C., Verardi, R., Wang, K., Zhou, J., Zhou, T., Georgiou, G., Alam, S.M., Haynes, B.F., Rolland, M., Matyas, G.R., Polonis, V.R., McDermott, A.B., Douek, D.C., Shapiro, L., Tovanabutra, S., Michael, N.L., Mascola, J.R., Robb, M.L., Kwong, P.D. and N.A. Doria-Rose, “Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual,” Immunity 50:677-691 (2019) PMID: 30876875 DOI: 10.1016/j.immuni.2019.02.008

Lee, J., Paparoditis, P., Horton, A.P., Frühwirth, A., McDaniel, J.R., Jung, J., Boutz, D.R., Hussein, D.A., Tanno, Y., Pappas, L., Ippolito, G.C., Corti, D., Lanzavecchia, A. and G. Georgiou, “Persistent Antibody Clonotypes Dominate the Serum Response to Influenza over Multiple Years and Repeated Vaccinations,” Cell Host Microbe 25:367-376 (2019) PMID: 30795981 DOI: 10.1016/j.chom.2019.01.010

Manso, T.C., Groenner-Penna, M., Minozzo, J.C., Antunes, B.C., Ippolito, G.C., Molina, F., and Felicori, L.F., “Next-generation sequencing reveals new insights about gene usage and CDR-H3 composition in the horse antibody repertoire,” Mol. Immunology 105:251-259 (2019) PMID: 30562645


Johnson, E.L., Doria-Rose, N.A., Gorman, J., Bhiman, J.N., Schramm, C.A., Vu, A.Q., Law, W.H., Zhang, B., Bekker, V., Karim, S.S.A., Ippolito, G.C., Morris, L., Moore, P.L. Kwong, P.D., Mascola, J.R. and G. Georgiou, “Sequencing HIV-neutralizing antibody exons and introns reveals detailed aspects of lineage maturation” Nature Communications 9(1):4136 (2018) PMID: 30297708 DOI:10.1038/s41467-018-06424-6

Saphire, E.O., Schendel, S.L., Fusco, M.L., Gangavarapu, K., Gunn, B.M., Wec, A.Z., Halfmann, P.J., Brannan, J.M., Herbert, A.S., Qiu, X., Wagh, K., He, S., Giorgi, E.E., Theiler J, Pommert KBJ, Krause TB, Turner HL, Murin CD, Pallesen J, Davidson E, Ahmed R, Aman MJ, Bukreyev A, Burton DR, Crowe JE Jr, Davis CW, <u>Georgiou G</u>, Krammer F, Kyratsous CA, Lai JR, Nykiforuk C, Pauly MH, Rijal P, Takada A, Townsend AR, Volchkov V, Walker LM, Wang CI, Zeitlin L, Doranz BJ, Ward AB, Korber B, Kobinger GP, Andersen KG, Kawaoka Y, Alter G, Chandran K, Dye JM; Viral Hemorrhagic Fever Immunotherapeutic Consortium, “Systematic Analysis of Monoclonal Antibodies against Ebola Virus GP Defines Features that Contribute to Protection,” Cell 2018 Aug 9;174(4):938-952.e13. doi: 10.1016/j.cell.2018.07.033. PMID: 30096313

Triplett, T.A., Garrison, K.C., Marshall, N., Donkor, M., Blazeck, J., Lamb, C., Qerqez A., Dekker, J.D., Tanno, Y. Lu, W-C., Karamitros, S.K., Ford, K., Tan, B., Zhang, M., McGovern, K., Coma, S., Kumada, Y., Yamany, M.S., Sentandreu, S., Fromm, G., Tiziani, S., Schreiber, T.H., Manfredi, M., Ehrlich, L.I.R., Stone E., and G. Georgiou, “Reversal of IDO-Mediated Cancer Immune Suppression by Systemic Kynurenine Depletion with a Therapeutic Enzyme,” Nature Biotechnology doi: 10.1038/nbt.4180

Lee, J. and G. Georgiou, “High Affinity IgA Against Microbial Antigens” Nature Immunology 2018 19:514-515 (2018) PMID: 29777208

Liu, Y., McDaniel, J.R., Khan, S., Campisi, P., Propst, E.J., Holler, T., Grunebaum, E., Georgiou, G., Ippolito, G.C. and Ehrhardt, G.R.A., “Antibodies Encoded by FCRL4-Bearing Memory B Cells Preferentially Recognize Commensal Microbial Antigens,” J Immunology 200:3962-3969 (2018) PMID: 29703863

Chen, Y.Q., Wohlbold, T.J., Zheng, N.Y., Huang, M., Huang, Y., Neu, K.E., Lee, J., Wan, H., Rojas. K.T., Kirkpatrick., E., Henry, C., Palm, A.E., Stamper, C.T., Lan, L.Y., Topham, D.J., Treanor, J., Wrammert, J., Ahmed, R., Eichelberger, M.C., Georgiou, G, Krammer, F. and Wilson, P.C., “Influenza Infection in Humans Induces Broadly Cross-Reactive and Protective Neuraminidase-Reactive Antibodies,” Cell 173:417-429 (2018). PMID: 29625056

  • Commentaries and News Stories: Covered by 9 scientific and mainstream media publications

McDaniel, J.R., Pero, S.C., Voss, W.N., Shukla, G.S., Sun, Y., Schaetzle, S., Lee, C.H., Horton, A.P., Harlow, S., Gollihar, J., Ellefson, J.W., Krag, C.C., Tanno, Y., Sidiropoulos, N., Georgiou, G., Ippolito, G.C. and Krag, D.N., “Identification of Tumor-Reactive B Cells and Systemic IgG in Breast Cancer Based on Clonal Frequency in the Sentinel Lymph Node,” Cancer Immunology Immunother 67:729-738 (2018) PMID: 29427082

Wang, B., DeKosky, B.J., Timm, M.R., Lee, J., Normandin, E., Misasi, J., Kong, R., McDaniel, J.R., Delidakis, G., Leigh, K.E., Niezold, T., Choi, C.W., Viox, E.G., Fahad, A., Cagigi, A., Ploquin, A., Leung, K., Yang, E.S., Kong, W.P., Voss, W.N., Schmidt, A.G., Moody, M.A., Ambrozak, D.R., Henry, A.R., Laboune, F., Ledgerwood, J.E., Graham, B.S., Connors, M., Douek, D.C., Sullivan, N.J., Ellington, A.D., Mascola, J.R. and G. Georgiou, “Functional Interrogation and Mining of Natively Paired Human VH:VL Antibody Repertoires,” Nature Biotechnology 36:152-155 (2018) PMID: 29309060


McDaniel, J.R., Ippolito G.C. and G. Georgiou, “Mapping the Secrets of the Antibody Pool,” Nature Biotechnology 35:921-922. (2017) PMID: 28892077.

Jang, S-H., Gregersen, P.K., Georgiou, G., Diamond, B., “Increased Cathepsin S in Dendritic Cells Alters TFH Repertoire and Contributes to Lupus,”, Nature Immunology 18:1016-1024 (2017) PMID: 28692065

Cotham, V.C., Horton, A.P., Lee, J., Georgiou, G. and Brodbelt, J.S., “Middle-down 193-nm Ultraviolet Photodissociation for Unambiguous Antibody Identification and its Implications for Immunoproteomic Analysis,” Anal. Chem. 89:6498-6504 (2017) PMID: 28517930

Lee, C.-H., Romain, G., Yan, W., Watanabe, W., Charab, W., Todorova, B., Lee, Y., Triplett, K., Donkor, M., Lungu, O.I., Lux, A., Marshall, N., Lindorfer, M.A., Richard-Le Goff, O., Balbino, B., Kang, T.H., Tanno, H., Delidakis, G., Alford, C., Taylor, R.P., Nimmerjahn, F., Varadarajan, N., Bruhns, P. Zhang, Y.J.  and G. Georgiou, “IgG Fc Domains that Bind C1q but not Effector Fcγ Receptors Delineate the Significance of Complement-Dependent Cell-Mediated Cytotoxicity and Phagocytosis in Antibody Function,” Nature Immunology 18:889-898 (2017) DOI: 10.1038/ni.3770

Lau, D., Lan, L.Y., Andrews, S.F., Henry, C., Thatcher Rojas, K., Neu, K.E., Huang, Y., DeKosky, B., Palm, A.E., Ippolito, G.C., Georgiou, G., Wilson, P., “Low CD21 expression defines a population of recent germinal center graduates primed for plasma cell differentiation” Science Immunology Vol. 2, Issue 7 (2017) DOI: 10.1126/sciimmunol.aai8153

Steiniger, S.C., Glanville, J., Harris, D.W., Wilson, T.L., Ippolito, G.C., and Dunham, S.A., “Comparative analysis of the feline immunoglobulin repertoire,” Biologicals 46:81-87 (2017) PMID: 28131552

Williams, L.D., Ofek, G., Schatzle, S., McDaniel, J.R., Lu, X., Nicely, N.I., Wu, L., Lougheed, C.S., Bradley, T., Louder, M.K., McKee, K., Bailer, R.T., O’Dell, S., Georgiev, I.S., Seaman, M., Parks, R., Marshall, D., Anasti, K., Yang, G., Nie, X., Tumab, N., Wiehe, K., Wagh, K., Korber, B., Kepler, T., Alam, S.M., Morris, L., Kamanga, G., Choen, M.S., Bonsignori, M., Xia, S., Montefiori, D., Kelsoe, G., Gao, F., Mascola, J.R., Moody, M.A., Saunders, K.O., Liao, H., Tomaras, G.D., Georgiou, G., Haynes, B.F., “Potent and broad HIV-neutralizing antibodies in memory B cells and plasma”, Science Immunology 27 Jan 2017: Vol. 2, Issue 7. DOI: 10.1126/sciimmunol.aal2200

Li, Q., Yi, L., Hoi, K.H., Marek, P., Georgiou, G. and Iverson, B.L., “Profiling Protease Specificity: Combining Yeast ER Sequestration Screening (YESS) with Next Generation Sequencing,” ACS Chem. Biol. (2017) PMID: 27977123

Cramer, S.L., Saha, A., Liu, J., Tadi, S., Tiziani, S., Yan, W., Triplett, K., Lamb, C., Alters, S.E., Rowlinson, S., Zhang, Y., Keating, M.J., Huang, P., DiGiovanni, J., Georgiou, G., and E. Stone, “Systemic Depletion of Serum L-Cyst(e)ine with an Engineering Human Enzyme Mediates Potent Induction of ROS Leading to Selective Cancer Ablation,” Nature Medicine 23, 120-127, doi: 10.1038/nm.4232 (2017) PMID: 27869804


Wu, G.C., Cheung, N.V., Georgiou , G., Marcotte, E.M., Ippolito, G.C., “Temporal Stability and Molecular Persistence of the Bone Marrow Plasma Cell Antibody Repertoire,” Nature Communications 7:13838  (2016) PMID: 28000661

Lee, J., Boutz, D.R., Chromikova, V., Joyce, M.G., Vollmers, C., Leung, K., Horton, A.P., DeKosky, B.J., Lee, C.H., Lavinder, J.J., Murrin, E.M., Chrysostomou, C., Hoi, K.H., Tsybovsky, Y., Thomas, P.T., Druz, A., Zhang, B., Zhang, Y., Wang, L., Kong, W.P., Park, D., Popova, L.I., Dekker, C.L., Davis, M.M., Bloom, C.E., Ross, T.M., Ellington, A.D., Wilson, P.C., Marcotte, E.M., Mascola, J.R., Ippolito, G.C., Krammer, F., Quake, S.R., Kwong, P.D. and Georgiou, G., “Quantitative, Molecular-Level Analysis of the Serum Antibody Repertoire Reveals Unanticipated Features of the Response to Influenza Vaccine,” Nature Medicine 22:1456-64 (2016) PMID: 27820605

  • Commentaries and News Stories: Covered by >20 scientific and mainstream media publications;
    e.g. Drug Discovery & Development:
  • Raymond, D., Stewart, S., Jiwon, L., Ferdman, J., Bajic, G., Khoi, T.D., Ernandes, M.J., Pirada, S., Settembre, Dormitzer, P.R., Del Giudice, G., Finco, O., Kang, T.H., Ippolito, G.C., Georgiou, G., Kepler, T.M., Haynes, B.F., Moody, M.A., Liao, H-X., Schmidt, A.G. and S.C. Harrison, “Influenza Immunization Elicits Hemagglutinin Receptor-Site Antibodies Specific for an Egg-Adapted Vaccine Strain,” Nature Medicine 22:1465-69 (2016) PMID: 27820604

    Wang, B., Lee, C.H., Johnson, E.L., Kluwe, C.A., Cunningham, J.C., Tanno, H., Crooks, R.M., Georgiou. G. and A.D. Ellington,  “Discovery of High Affinity Anti-Ricin Antibodies by B Cell Receptor Sequencing and by Yeast Display of Combinatorial VH:VL Libraries from Immunized Animals“, MAbs. 25:1-10. (2016) PMID: 27224530

    DeKosky, B.J., Lungu, O., Parek, D., Johnson, E.L, Charab, W.,  Chrysostomou, C., Kuroda, D., Ellington, A.D., Ippolito, G.C., Gray, J.J. and G. Georgiou, “Large-Scale Sequence and Structural Comparisons of Human Naïve and Antigen-Experienced Antibody Repertoires,” Proc. Natl. Acad. Sci. USA 113:E2636-45 (2016) PMID: 27114511

    Biswas, P., Chavali, V.R., Agnello, G., Stone, E., Chakarova, C., Duncan, J.L., Kannabiran, C., Homsher, M., Bhattacharya, S.S., Naeem, M.A., Kimchi, A., Sharon, A., Iwata, T., Riazuddin, S. , Reddy, G.B., Hejtmancik, J.F., Georgiou, G., Riazuddin, S.A. and R. Ayyagari, “A Missense Mutation in ASRGL1 is Involved in Causing Autosomal Recessive Retinal Degeneration” Hum Mol Genet. pii: ddw113. (2016) PMID: 27106100

    Breski, R.J. and G. Georgiou, “Immunoglobulin Isotype Knowledge and Application to Fc Engineering, “ Curr. Opin. Immunol. 40:62-69 (2016). PMID: 27003675

    Dekker, J.D., Park, D., Shaffer, A.L., Kohlhammerc, H., Deng, W., Lee, B.-K., Ippolito, C.C., Georgiou, G., Iyer, V.R., Staudt, L.M., Tucker, H.O., “Subtype Specific Addiction of the Activated B Cell Subset of Diffuse Large B Cell Lymphoma to FOXP1,” Proc. Natl. Acad. Sci. USA 113:E577-86 (2016). PMID: 26844430

    McDaniel, J.R., DeKosky, B., Tanno, H., Ellington, A.D. and G. Georgiou, “Ultra- high-throughput sequencing of the immune receptor repertoire from millions of lymphocytes,” Nature Protocols 11:429-42 (2016). PMID: 26787899

    Murzabaev, M., Kojima, T., Mizoguchi, T., Kobayashi, I., DeKosky, B.J., Georgiou, G., H. Nakano, “Handmade Microfluidic Device for Biochemical Applications in Emulsion,” J Biosci Bioeng. 121:471-6 (2016) PMID: 26386750

    Gorman, J., Soto, C., Yang, M.M., Davenport, T.M, Guttman, M., Bailer, R.T., Chambers, M., Chuang, G.Y., DeKosky, B.J., Doria-Rose, N.A., Druz, A., Ernandes, M.J., Georgiev, I.S., Jarosinski, M.C., Joyce, M.G., Lemmin, T.M., Leung, S., Louder, M.K., McDaniel, J.R., Narpala, S., Pancera, M., Stuckey, J., Wu, X., Yang, Y., Zhang, B., Zhou, T., Program, N.C., Mullikin, J.C., Baxa, U., Georgiou, G., McDermott, A.B., Bonsignori, M., Haynes, B.F., Moore, P.L., Morris, L., Lee, K.K., Shapiro, L., Mascola, J.R., Kwong, P.D., “Structures of HIV-1 Env V1V2 with Broadly Neutralizing Antibodies Reveal Commonalities that Enable Vaccine Design,” Nat Struct. Mol Biol. 23:81-90 (2016). PMID: 26689967


    Quandt, E., Gollihar, J., Blount, Z., Ellington, A.D., Georgiou, G. and J.E Barrick, “Fine-Tuning Citrate Synthase Flux Potentiates and Refines Metabolic Innovation in the Lenski Evolution Experiment,” Elife doi: 10.7554/eLife.09696 (2015) PMID: 26465114.

    Burrage, L.C., Sun, Q., Elsea, S.H., Jiang, M.N., Nagamani, S.C., Frankel, A.E., Stone, E., Alters, S.E., Johnson, D.E., Rowlinson, S.W., Georgiou, G, Members of the Urea Cycle Disorders Consortium and B.H. Lee, “Human Recombinant Arginase Enzyme Reduces Plasma Arginine in Mouse Models of Arginase Deficiency,” Hum Mol Genet. 24:6,417-27 (2015). PMID: 26358771

    Kerr, S.A., Jackson, E.L., Lungu, O.I., Meyer, A.G., Demogines, A., Ellington, A.D., Georgiou, G., Wilke, C.O. and S. L. Sawyer, “Computational and Functional Analysis of the Virus-Receptor Interface Reveals Host Range Trade-offs in New World Arenaviruses,” J. Virol. 89:11643-53 (2015) PMID: 26355089.

    Wang, B., Kluwe, C.A., Lungu, O.I., DeKosky, B.J., Kerr, S.A., Johnson, E.L., Jung, J., Rezigh, A.B., Carroll1, S.M., Reyes, A.N., Bentz, J.R., Villanueva, I., Altman, A.L., Davey, R.A., Ellington, A.D. and G. Georgiou, “Facile Discovery of a Diverse Panel of Anti-Ebola Virus Antibodies by Immune Repertoire Mining,” Scientific Reports 5:13926. doi: 10.1038/srep13926 (2015) PMID: 26355042.

    Wine, Y., Horton, A.P., Ippolito, G.C. and Georgiou G., “Serology in the 21st Century: the Molecular-Level Analysis of the Serum Antibody Repertoire,” Curr Opin Immunol. 35:89-97 (2015) PMID: 26172290

    Yi, L., Taft, J.M., Li, Q., Gebhard, M.C., Georgiou, G. and Iverson, B.L., “Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast” Methods Mol Biol. 1319:81-93 (2015) PMID: 26060071

    Chrysostomou, C., Quandt, E. M., Marshall, N.M., Stone, E. and Georgiou G.  “An Alternate Pathway of Arsenate Resistance in E. coli Mediated by the Glutathione-S-Transferase GstB,” ACS Chem Bio (2015) PMID: 25517993

    DeKosky, B.J., Kojima, T., Rodin, A., Charab, W., Ippolito, G.C., Ellington, A.D. and G. Georgiou, “Ultra-High Throughput Antibody VH:VL Sequencing Using a Novel Single-Cell Flow Focusing Technology,” Nature Medicine 21:86-91(2015) PMID: 25501908

    Lavinder, J.J., Horton, A.P., Georgiou, G and Ippolito G.C., “Next-Generation Sequencing and Protein Mass Spectrometry for the Comprehensive Analysis of Human Cellular and Serum Antibody Repertoires,” Curr Opin Chem Biol.  24:112-120 (2015) PMID: 25461729


    Kelton, W., Mehta, N., Charab, W., Lee, J., Kojima, T., Kang, T.-H. and G. Georgiou, “IgGA: A “Cross-Isotype” Engineered Human Fc Antibody Domain that Displays Both IgG-like and IgA-like Effector Functions,”  Chem & Biol. 21:1603-9 (2014) PMID: 25500223

  • Chem & Bio featured Article
  • Romain G., Senyukov, R.R., Merouane, A., Kelton, W., Liadi, M. A., Georgiou, G., Roysam, B., Lee, D.A. and N. Varadarajan, “Antibody Fc-engineering Improves Frequency and Promotes Kinetic Boosting of Serial Killing Mediated by Natural Killer Cells,” Blood (2014) pii: blood-2014-04-569061. [Epub ahead of print] PMID: 25232058.DeKosky, B.J., Kojima, T., Rodin, A., Charab, W., Ippolito, G.C., Ellington, A.D. and G. Georgiou, “Ultra-High Throughput Antibody VH:VL Sequencing Using a Novel Single-Cell Flow Focusing Technology,” Nature Medicine (accepted)Lavinder, J.J., Hoi, K.H., Reddy, S.T., Wine, Y. and G. Georgiou, “Systematic Characterization and Comparative Analysis of the Rabbit Immunoglobulin Repertoire”, PLoS One 9:e101322 (2014) PMID: 24684310.Boutz, D.R., Horton, A.P., Wine, Y., Lavinder, J., Georgiou, G. and E.M. Marcotte,” “Proteoimic Identification of Monoclonal Antibodies from Serum, Anal. Chem. (2014) PMID: 24684310.Mroczek, E.S., Ippolito, G.C., Rogosch, T., Hoi, K.H., Hwangpo, T.A., Brand, M.G., Zhuang, Y., Liu, C.R., Schneider, D.A., Zemlin, M., Brown, E.E., Georgiou, G., Schroeder, HW Jr. “Differences in the Composition of the Human Antibody Repertoire by B Cell Subsets in the Blood,” Front.  Immunol. 19:96 (2014) PMID: 24678310.Laserson, U., Vigneault, F., Gadala-Maria, D., Yaari, G., Uduman, M., Kasvin-Felton, J., Kelton, W., Jung, S.-T., Liu, Y.-P., Lasserson, J., Chari, R., Lee, J., Bachelet, I., Hickey, B., Lieberman-Aiden, E., Hanczaruk, B., Simen, B.B., Egholm, M., Koller, D., Georgiou, G., Kleinstein, S., George Church,  “High-Resolution Antibody Dynamics of Vaccine-Induced Immune Responses” Proc. Natl. Acad. Sci. USA 111:4928-4933(2014) PMID: 24639495.Doria-Rose, N.A., Schramm, C.A., Gorman, J., Moore, P.L., Bhiman, J.N., DeKosky, B., Ernandes, M.J., Georgiev, I.S., Kim, H.J., Pancera, M., Staupe, R.P., Altae-Tran, H.R., Bailer, R.T., Crooks, E.T., Cupo, A., Druz, A., Garrett, J.N., Hoi, K.H., Kong, R., Louder, M.K., Longo, N.S., McKee, K., Nonyane, M., O’Dell, S., Roark, R.S., Rudicell, R.S., Schmidt, S.D., Sheward, D.J., Soto, C., Wibmer, C.K., Yang, Y. Zhang, Z., Mullikin, J.C., Binley, J.M., Sanders, R.W., Wilson, I.A., Moore, J.P., Ward, A.B., Georgiou, G., Williamson, C., Abdool Karim, S.S., Morris, L., Kwong, P.D., Shapiro, L., Mascola, J.R., “Developmental Pathway for Potent V1V2-Directed HIV-1-Neutralizing Antibodies,”  Nature509:55-62 (2014)PMID: 24590074

  • Commentaries: MedicalXpress:; Science Newsline:;  Medical Nees Today:;
  • Selected as a “Recommended” paper:
  • Georgiou G., Ippolito, G., Beuasang, G., Busse, C.E., Wardemann, H. and S.R. Quake, “The Promise and Challenge of High-throughput Sequencing of the Antibody Repertoire,” Nature Biotechnol. 32:158-68 (2014).  PMID: 24441474.Quandt, E.M., Deatherage, D.E., Ellington, A.D., Georgiou, G. and J. E. Barrick, “Recursive genome-wide recombination and sequencing reveals a key refinement step in the evolution of a metabolic innovation in E. coli,”  Proc. Natl. Acad. Sci. USA 111:2217-22 (2014) PMID: 24379390

  • Commentaries:;
    National Geographic:; Proc. Natl. Acad. Sci. USA Highlight:.; Proc. Natl. Acad. Sci. USA Commentary:; Le Scienze:;
  • Lavinder, J., Wine, Y., Giesecke, C., Ippolito, G.C., Horton, A.P., Hoi, K.-H., DeKosky, B.J., Ellington, A.D., Dörner, T., Marcotte, E.M., Boutz, D.R. and G. Georgiou,  “Dynamics, Functionality and Discordance of the Human Serum Antibody and BCR Repertoires Following Immunization” Proc. Natl. Acad. Sci. USA 111:2259-64(2014) PMID: 24469811

  • Commentaries: Highlight, Proc. Natl. Acad. Sci:
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    Tanios, R., Bekdash, A., Kassab. E., Stone, E., Georgiou, G., Frankel, A.E., Abi-Habib, R.J. “Human Recombinant Arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-Induced Arginine Depletion is Selectively Cytotoxic to Human Acute Myeloid Leukemia Cells,” Leuk Res. 37:1565-71 (2013) PMID: 24018014.Paley, O., Agnello, G., Cantor, J., Yoo, T.H., Georgiou, G., Stone E.M., “GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria,” Methods Mol. Biol. 978:31-44 (2013) PMID: 23423887DeKosky, B.J., Ippolito, G.C., Deschner, R.P., Lavinder, J.J., Rawlings, M.P., Varadarajan, N., Wine, Y., Giesecke, C., Dörner, T., Andrews, S.F., Wilson, P.C., Hunicke-Smith, S.P., Wilson, C.P., Ellington, A.D. and G. Georgiou, “High Throughput Determination of the Antibody VH:VL Paired Repertoires,” Nature Biotechnol. 31:166-69(2013) PMID: 23334449Needham, B.D., Carroll, S., Giles, D.K., Georgiou, G., Whiteley, M., and Trent, M.S.  “Modulating the Innate Immune Response by Combinatorial Engineering of Endotoxin” Proc. Natl. Acad. Sci. USA 110:1464-69 (2013) PMID: 23297218

  • Commentaries Science:; The Scientist:
  • Selected as a “Recommended” paper:
  • Wine, Y., Boutz, D., Lavinder, J.J., Horton, A., Miklos, A.E., Hughes, R.A., Jung, S.T, Murrin E.M., Ellington, A.D., Marcotte, E.M. and G. Georgiou,  “Molecular Deconvolution of the Monoclonal Antibodies that Comprise the Polyclonal Serum Response,” Proc. Natl. Acad. Sci. USA 110: 2993-8(2013) PMID: 23382245Carroll, S. and G. Georgiou,“Antibody-Mediated Inhibition of Human C1s and the Classical Complement Pathway, “Immunobiology doi:pii: S0171-2985(13)00006-5. 10.1016/j.imbio.2013.01.001 (2013).Mittal, J., Yoo, T-H., Georgiou, G. and T.M. Truskett, “Structural Ensemble of an Intrinsically Disordered Polypeptide” J Phys Chem B. 117:118-24 (2013) PMID: 23205890Jung, S. T., Kelton, W., Kang, T. H.; Ng, D., Anderssen, J. T.; Sandlie, I., Sarkar, C., G. Georgiou,
    “Effective Phagocytosis of Low Her2 Tumor Cell Lines with Engineered, Aglycosylated IgG Displaying High FcγRIIa Affinity and Selectivity” ACS Chem. Bio. 8:368-75 (2013) PMID: 23030766


    Saggy, I., Wine, Y., Georgiou G. and Benhar, I., “Antibody Isolation from Immunized Animals: Comparison of Phage Display and Antibody Discovery via V gene Repertoire Mining” PEDS (2012) PMID: 22988130Stone, E.M., Paley, O., Hu, J., Ekerdt, B.; Cheung, N.-K. and G., George, “De novo Engineering of a Human Methionine-γ-Lyase for Systemic L-Met Depletion Cancer Therapy,” ACS Chem. Bio. (2012) PMID: 22963240

  • Commentary in Chem & Eng News
  • Romero, P.A., Stone, E.M., Lamb, C., Chantranupong, L., Krause, A., Miklos, A.E., Hughes, R.A., Fechtel, B., Ellington, D.A., Arnold, F.H. and G. Georgiou, “SCHEMA-Designed Variants of Human Arginase I and II Reveal Sequence Elements Important to Stability and Catalysis”, ACS Synth. Biol. 1:221-28 (2012) PMID: 22737599Li W., Cantor, J., Yogesha, S.D.,  Yang, S., Chantranupong, L., Liu, J.Q., Agnello, G., G. Georgiou, Stone E.M.,  and Y. Zhang,“Uncoupling Intramolecular Processing and Substrate Hydrolysis in the N-terminal Hydrolase hASRGL1 by Circular Permutation” ACS Chem. Bio. Epub ahead of print (2012) PMID: 22891768Borrok, J.M., Jung, S.T., Kang, T.H. ; Monzingo, A. and G. Georgiou, Revisiting the Role of Glycosylation in the Structure of Human IgG Fc”  ACS Chem. Bio (2012) PMID: 22747430Rani, M., Bolles, M., Donaldson, E.F., Van Blarcom, T.,  Baric, R., Iverson, B.L. and G. Georgiou “Increased Antibody Affinity Confers Broad In Vitro Protection Against Escape Mutants of SARS-CoV”  J. Virology 86:9113-21 (2012)  PMID: 2269665Skretas, G.  Makino, T. Varadarajan N.,  Pogson M. and G. Georgiou,  “Multi-Copy Genes that Enhance the Yield of Mammalian G Protein-Coupled Receptors in Escherichia coliMetabolic Eng. 14:591-602 (2012) PMID: 22609824Mauldin, J.P., Zeinali, I., Kleypas, K., Woo, J.H., Blackwood, R.S., Jo, C.H., Stone, E.M., Georgiou G., Frankel A. E.,Transl. Oncology 5:26-31 (2012) PMID: 22348173Ippolito, G.C.,  Hoi K.H., Reddy S.T., Carroll S.M., Ge X., Rogosch T., Zemlin M., Shultz L.D., Ellington A.D., Vandenberg C.L., and G. Georgiou, “Antibody Repertoires in Humanized NOD-scid-IL2Rγnull Mice
    and Human B Cells Reveals Human-like Diversification and Tolerance Checkpoints in the Mouse” PLoS One7:e35497 (2012) PMID: 2255816Miklos, A.E., Kluwe, C., Der. B.S., Pai, S., Sircar, A., Hughes, R.A., Berrondo, M., Xu, J., Codrea, V., Buckley,
    P.E., Calm, A.M., Walsh, H.S., Warner, C.R., Zacharko, M.A., Carney, J.P., Georgiou, G., Kuhlman, B. and A.D. Ellington,  “Structure-Based Design of Supercharged, Highly Thermostable Antibodies,”  Chem. & Biol.  19:449-55 (2012) PMID: 22520751Yoo, T-H., Pogson, M., Iverson, B.L. and G. Georgiou,  “Directed Evolution of Highly Selective Proteases Using a Novel FACS Based Screen that Capitalizes on the p53 Regulator MDM2” ChemBiochem 3:649-53(2012) PMID: 22334509Cantor,  J.R., Panayotou, V., Agnello, G., Stone. E.M. and G. Georgiou, “Engineering Reduced Immuno–genicity Enzymes for Amino Acid Depletion Therapy in Cancer,”  Methods Enzymol. 502:291-312 (2012). PMID:22208990Agrawal, V., Boyer, A.L., Woo, J-H., Mauldin,J.P., Chanhee, J. Stone, E.M., Georgiou, G. and Arthur E. Frankel, ” Cytotoxicity of Human Recombinant Arginase I [Co] PEG5000 in the Presence of L-Citruline is Dependent on Arginosuccinate Synthetase Expression in Human Cells”Anti-Cancer Drugs 23:51-64 (2012)PMID:21955999Stone, E. M., Chantrranupong L., Gonzalez, C., O’Neal, J., Rani, M., VanDenBerg, C. and G. Georgiou, “Strategies for Optimizing the Serum Persistence of Engineered Human Arginase I for Cancer Therapy,” J. Control. Release58L171-9 (2012) PMID 22001609.