Translational Protein Therapeutics Lab

Current Group Members

/by

Current Members of the Lab


Ranya Al-Khaledy

Graduate Research Assistant
PhD Candidate, Chemical Engineering, UT Austin
M.S., Chemistry, University of Oregon
B.S., Chemical Engineering, Oregon State University
alkhaler[at]utexas.edu
she/her/hers


Alessandra Araujo, Ph.D.

Research Associate
Ph.D., Immunology, University of Rochester
M.S., Animal Physiology, Southern Illinois University
B.S., Molecular Biology, University of Southern Florida
alessandra[at]austin.utexas.edu


Nick Azzi

Undergraduate Research Assistant
Chemical Engineering


Mark Badeaux, Ph.D.

Research Associate
Ph.D., Molecular Carcinogenesis, University of Texas Health Science Center/MD Anderson Cancer Center
B.S., Microbiology, UT Austin
m.badeaux[at]utexas.edu


Sarah Bayne, PharmD

Graduate Research Assistant
Co-advised w/ Dr. Everett Stone
PhD Candidate, Molecular Biosciences, UT Austin
Pharm.D., Drake University
B.A., Biochemistry, Augustana College


Sarita Bhetawal, M.S.

Research Engineering/Scientist Associate III
M.S. Biomedical Engineering, University of Texas Arlington/UT Southwestern Medical Center
B.S. Biotechnology/Biochemistry, Minnesota State University
sarita.bhetawal[at]austin.utexas.edu


Kristin Connelly

Graduate Research Assistant
Co-advised w/ Dr. Jason Lavinder
Co-advised w/ Dr. Allison Seeger
PhD Candidate, Biomedical Engineering, UT Austin
B.S., Biomedical Engineering, Boston University
kmconnelly[at]utexas.edu
she/her/hers


Elisa Contreras

Graduate Research Assistant
PhD Candidate, Molecular Biosciences, UT Austin
Co-advised w/ Dr. Jason Lavinder
B.S., Biotechnology, Technological Institute of Santo Domingo
econtreras[at]my.utexas.edu


George Georgiou, Ph.D.

Professor
Ph.D., Chemical Engineering, Cornell University, 1987
gg[at]che.utexas.edu

More information here


Luke Hebert

Graduate Research Assistant
Co-advised w/ Dr. Allison Seeger
PhD Candidate, Molecular Biosciences, UT Austin
B.S., Bioinformatics, University of St. Thomas, Houston
luke.hebert[at]austin.utexas.edu


Bart Herpers

Graduate Research Assistant
PhD Candidate, Biomedical Engineering, UT Austin
M.S., Drug Innovation, University Utrecht
B.S., Biotechnology, Wageningen University & Research
b.m.herpers[at]utexas.edu


Tae Hyun Kang, Ph.D.

Visiting Professor, Kookmin University
Ph.D. Biomedical Engineering, UT Austin
M.A., Cell and Molecular Biology, UT Austin
M.S., Biotechnology, Yonsei University
B.S., Biotechnology, Yonsei University


Dae Sung Kim

Graduate Research Assistant
PhD Candidate, Biomedical Engineering, UT Austin
M.S., Chemical Engineering, University of Waterloo
B.S., Biosystem Engineering, University of Manitoba
dkim2[at]utexas.edu


Elaine Knudson

Undergraduate Research Assistant
Chemical Engineering


Sean Knudson

Graduate Research Assistant
PhD Candidate, Molecular Biosciences, UT Austin
B.S., Biochemistry, The University of Texas at Austin
seanknudson[at]utexas.edu


Sanghwan Ko, Ph.D.

Postdoctoral Fellow
Ph.D., Biochemistry, Kookmin University
M.S., Biochemistry, Kookmin University
B.S. Bio and Nano Chemistry, Kookmin University
sanghwanko[at]utexas.edu


Jason Lavinder, Ph.D.

Research Assistant Professor
Ph.D., Biochemistry, The Ohio State University
B.S., Biology, Wright State University
jlavinder[at]utexas.edu


Emily Lewis

Graduate Research Assistant, Dr. Everett Stone
PhD Candidate, Molecular Biosciences, UT Austin


Victoria Longo

Undergraduate Research Assistant


Jeffrey Marchioni, Ph.D.

Postdoctoral Fellow
Ph.D., Biomedical Engineering, UT Austin
B.S., Bioengineering, Northeastern University
jeffrey.marchioni[at]utexas.edu
he/his/him


Christina Martins, Ph.D.

Postdoctoral Fellow w/ Dr. Jason Lavinder
Ph.D., Biochemistry and Immunology, Universidade Federal de Minas Gerais
M.S., Biochemistry and Immunology, Universidade Federal de Minas Gerais
B.S., Biotechnology, Universidade Federal de Uberlandia
B.S., Biological Sciences, Centro Universitario de Patos de Minas
christina.martins[at]utexas.edu
she/her/hers


Ladan Mashouri

Graduate Research Assistant
Co-advised w/ Dr. Everett Stone
PhD Candidate, Molecular Biosciences, UT Austin
B.S., Genetics, University of Isfahan
M.S., Genetics, Shahrekord University
ladan.mashouri[at]utexas.edu


Lizzie Minnich, CRA

Senior Grants & Contracts Specialist
Operations & Strategic Management Lead for the Georgiou Lab
Assistant to Dr. Georgiou
eminnich[at]che.utexas.edu
she/her/hers


Weiwei Peng, Ph.D.

Postdoctoral Fellow
Ph.D., Biomolecular Spectrometry and Proteomics, University Utrecht
M.S., Biotechnology, TU Dresden, IHI Zittau, Germany
B.S., Wuhan University
weiwei.peng[at]austin.utexas.edu


Max Rodnick-Smith, Ph.D.

Research Associate w/ Dr. Everett Stone
Ph.D., Chemistry, The University of Oregon
maxrs[at]utexas.edu


Allison Seeger, Ph.D.

Senior Research Scientist
Ph.D., Medical Microbiology, Ludwig-Maximilians-Universitat,
Munich, Germany
allison.seeger[at]austin.utexas.edu


Maia Stern

Undergraduate Research Assistant
Biomedical Engineering


Everett Stone, Ph.D.

Research Associate Professor
Ph.D., Cellular & Molecular Biology, UT Austin
stonesci[at]utexas.edu

2017 Emerging Inventor of the Year


Anika Tahsin

Graduate Research Assistant
Co-advised w/ Dr. Jason Lavinder
PhD Candidate, Molecular Biosciences, UT Austin
M.S., Biochemistry and Molecular Biology, University of Dhaka, Bangladesh
B.S., Biochemistry and Molecular Biology, University of Dhaka, Bangladesh


Douglas Townsend

Graduate Research Assistant
Co-advised w/ Dr. Jason Lavinder
PhD Candidate, Molecular Biosciences, UT Austin
B.S., Chemistry, Northeastern University
douglas.townsend[at]utexas.edu


Willam N. Voss, Ph.D.

Postdoctoral Fellow
Ph.D., Molecular Biosciences, UT Austin
B.A. Biology, Reed College
wnv[at]utexas.edu


Makiko Watanabe, DVM, PhD

Senior Research Scientist
D.V.M., Veterinary Medicine, Rakuno Gakuen University
Ph.D., Medicine, Osaka University
makiko.watanabe[at]austin.utexas.edu


Chloe Wilk

Undergraduate Research Assistant
Neuroscience


Rocio Zapata-Bustos, Ph.D.

Laboratory Manager
Research Engineering/Scientist Associate II
Ph.D., Molecular Biology, IPICYT, San Luis Potosí, México
M.S., Molecular Biology, Molecular Biology, IPICYT, San Luis Potosí, México
B.S., Pharmaco-biological Chemistry, IPICYT, San Luis Potosí, México
rocio.zapatabustos[at]austin.utexas.edu

The Institute Joins Universal Influenza Vaccine Consortium

/in /by

The National Institute of Allergy and Infectious Diseases (NIAID), part of the National Institutes of Health, has initiated the Collaborative Influenza Vaccine Innovation Centers (CIVICs) program, a new network of research centers that will work together in a coordinated, multidisciplinary effort to develop more durable, broadly protective and longer-lasting influenza vaccines. NIAID will provide up to approximately $51 million in total first-year funding for the program, which is designed to support the CIVICs program centers over seven years.

https://www.nih.gov/news-events/news-releases/nih-forms-new-collaborative-influenza-vaccine-research-network

Publications

/by

Publications

2025

Hebert, L.S., Pickens, W., Satterwhite, E., Soto, G.B., Pflaum, F.M., Zhan, M., Moody, M.A., Kain, J., Kirchenbaum, G.A., Ferguson, J.A., Langel, S.N., Ross, T.M., Salutto, G.A., Uno, N., Richardson, R.A., Georgiou, G., Lavinder, J.J., Ippolito, G.C and A. Seeger, “Gene Discovery and Expression Analysis of the B Cell Receptor Repertoire in the Domestic Ferret Model” Vaccine 64:127725. doi: 10.1016/j.vaccine.2025.127725 (2025) PMID: 40961800

McDaniel, J.R., Voss, W.N., Bowyer, G., Rush, S.A., Spencer, A.J., Bellamy, D., Ulaszewska, M., Goike, J., Gregory, S., King, C.R., McLellan, J.S., Hill, A.V.S., Georgiou, G., Ewer, K.J. and G.C. Ippolito “Repertoire, Function, and Structure of Serological Antibodies Induced by the R21/Matrix-M Malaria Vaccine” J. Exp. Med. 222:e20241908 (2025) PMID: 40719751 DOI: 10.1084/jem.20241908.

Park, J., Lindesmith, L.C., Olia, A.S., Costantini, V.P., Brewer-Jensen, P.D., Mallory, M.L., Kelley, C.E., Satterwhite, E., Longo, V., Tsybovsky, Y., Stephens, T., Marchioni, J., Martins, C.A., Huang, Y., Chaudhary, R., Zweigart, M., May, S.R., Reyes, Y., Flitter, B., Vinjé, J., Tucker, S.N., Ippolito, G.C., Lavinder, J.J., Snijder J., Kwong, P.D., Georgiou, G., Baric, R.S. “Broadly Neutralizing Antibodies Targeting Pandemic GII.4 Variants or Seven GII Genotypes of Human Norovirus” Science Transl. Med. 17(788):eads8214 (2025) PMID: 40043137 DOI: 10.1126/scitranslmed.ads8214

McGrath, J.J.C., Park, J., Troxell, C.A., Chervin, J.C., Li, L., Kent, J.R., Changrob, S., Fu, Y., Huang, M., Zheng, N-Y., Wilbanks, G.D., Nelson, S.A., Sun, J., Inghirami, G., Madariaga, M.L.L., Georgiou, G. and Wilson, P.C. “Mutability and Hypermutation Antagonize Immunoglobulin Codon Optimality” Mol. Cell 85:430-444 (2025) PMID: 39708804 DOI: 10.1016/j.molcel.2024.11.033

2024

Park, J., Bartzoka, F., von Beck, T., Li, Z-N., MishinaM., Hebert, L.S., Kain, J., Liu, F., Sharma, S., Cao, W., Eddins, D.J., Kumar, A., Kim, J.E., Lee, J., Wang, Y., Schwartz, E.A., Brilot, A.F., Satterwhite, E,, Towers, D.M., McKnight, E., Pohl, J., Thompson, T.G., Gaglani, M., Dawood, F.S., Naleway, A.L., Stevens, J., Kennedy, R.B., Jacob, J., Lavinder, J.L., Levine, M.Z., Gangappa, S., Ippolito, G.C. , Sambhara, S., and G. Georgiou, “Molecular Features of the Serological IgG Repertoire Elicited by Egg-based, Cell-based, or Recombinant HA-based Seasonal Influenza Vaccines: A Comparative Prospective Observational Cohort Study” Lancet Microbe Dec 9:100935 (2024). PMID: 39667375 DOI: 10.1016/j.lanmic.2024.06.002.

Araujo, A.M., Dekker, J.M., Garrison, K., Su, Z., Rhee , C., Hu, C., Lee, B.-K., Hurtado, D.O., Lee, L., Iyer, V.R., Ehrlich, L.I.R., Georgiou, G., Ippolito, G.C., Yi, S.S. and H.O. Tucker, “Lymphoid Origin of Intrinsically Activated Plasmacytoid Dendritic Cells in Mice,” Elife (2024) 13:RP96394. PMID: 39269281 DOI: 10.7554/eLife.96394

Karamitros, C.S., Murray, K., Kumada, Y., Johnson, K.A., D’Arcy, S. and G. Georgiou, “Distinct Mechanistic, Conformational and Substrate Selectivity Profiles Emerging in the Evolution of High Catalytic Activity Enzymes via Parallel Trajectories,” Nature Com.  15:7068 (2024) PMID: 39152129 DOI: 10.1038/s41467-024-51133-y

Voss, W.N., Mallory, M.A., Byrne, P.O., Marchioni, J.M., Knudson, S.A., Powers, J.M., Leist, S.R., Dadonaite, B., Townsend, D.R., Kain, J., Huang, Y., Satterwhite, E., Castillo, I.N., Mattocks, M., Paresi, C., Munt, J.E., Scobey, T., Seeger, A., Premkumar, L., Bloom, J.D., Georgiou, G., McLellan, J.S., Baric, R.S., Lavinder, J.J. and G.C. Ippolito, “Hybrid Immunity to SARS-CoV-2 Arises from Serological Recall of IgG Antibodies Distinctly Imprinted by Infection or Vaccination,” Cell Reports Medicine 5:101668 (2024) PMID: 39094579  DOI: 10.1016/j.xcrm.2024.101668

Singhal, S., Rao, A.S., Stadanlick, J., Bruns, K., Sullivan, N.T., Bermudez, A., Honig-Frand, A., Krouse, A., Arambepola, S., Guo, E., Moon, E.K., Georgiou, G., Valerius, T., Albelda, S.M. and E. B. Eruslanov, “Dual Role of Trogocytosis Mediated by Human Tumor-Associated Macrophages and Neutrophils in the Regulation of Anti-tumor Antibody Efficacy,” Cancer Research 84:1029-1047 (2024) PMID: 38270915 DOI: 10.1158/0008-5472.CAN-23-2135

2023

Goike, J., Hsieh, C.-L., Horton, A.P., Gardner, E.C., Zhou, L., Bartzoka, F., Wang, N., Javanmardi, K., Herbert, A., Abbassi, S., Xie, X., Xia, H., Shi, P.-Y., Renberg, R., Segall-Shapiro, T.H., Terrace, C.I, Wu, W., Shroff, R., Byrom, M., Ellington, A.D., Marcotte, E.M., Musser, J.M., Kuchipudi, S.V., Kapur, V., Georgiou, G., Weaver, S.C., Dye, J.M., Boutz, D.R., McLellan, J.S. and J.D. Gollihar, “SARS-COV-2 Omicron Variants Conformationally Escape a Rare Quaternary Antibody Binding Mode,” Communications Biology 11;6:1250 (2023) PMID: 38082099  DOI: 10.1038/s42003-023-05649-6

Lee, K., Park, J., Tanno, H., Georgiou, G., Diamond, B. and S.J. Kim, “Peripheral T Cell Activation, Not Thymic Selection, Expands the T Follicular Helper Repertoire in a Lupus-prone Murine Model” Proc. Natl. Acad. Sci U S A. 120:e2309780120 (2023) PMID: 37983487 DOI: 10.1073/pnas.2309780120

Weber, T., Dähling, S., Rose, S., Affeldt, P., Vanshylla, K., Ullrich, L., Gieselmann, L., Teipel, F., Gruell, H., Di Cristanziano, V., Kim, D.S., Georgiou, G., Koch, N., Kreer, C. and F. Klein, “Enhanced SARS-CoV-2 humoral immunity following breakthrough infection builds upon the preexisting memory B cell pool” Science Immunol. 8 (89):eadk5845 (2023) PMID: 37976348 DOI: 10.1126/sciimmunol.adk5845

Schommers, P., Kim, D.S., Schlotz. M., Kreer, C., Eggeling, R., Hake, A., Stecher, M., Park. J., Radford, C.E., Dingens, A.S., Ercanoglu, M.S., Gruell, H., Odidika, S., Dahlhaus, M., Gieselmann, L., Ahmadov, E., Lawong, R.Y., Heger, E., Knops, E., Wyen, C., Kümmerle, T., Römer, K., Scholten, S., Wolf, T., Stephan, C., Suárez, I., Raju, N., Adhikari, A., Esser, S. Streeck, H., Duerr, R., Nanfack, A.J., Zolla-Pazner, S., Geldmacher, C., Geisenberger, O., Kroidl, A., William, W., Maganga, L., Ntinginya, N.E., Georgiev, I.S., Vehreschild J.J., Hoelscher, M., Fätkenheuer, G., Lavinder, J.J., Bloom, J.D., Seaman, M.S., Lehmann, C., Pfeifer, N., Georgiou G and F. Klein “Dynamics and Durability of HIV-1 Neutralisation are Determined by Viral Replication, Nature Medicine 29: 2763–2774 (2023) PMID: 37957379 DOI: 10.1038/s41591-023-02582-3

Gjuka D., Adib, E., Garrison, K, Chen, J., Zhang, Y., Li, Y, Boutz, D., Lamb, C., Tanno, Y., Nassar, A., Zarif, T.E., Kale, N., Rakaee, M., Mouhieddine, T. H., Alaiwi, S.A., Gusev, A., Rogers, T., Gao, J., Georgiou, G., Kwiatkowski, D. J. and E. Stone, “Enzyme-Mediated Depletion of Methylthioadenosine Restores T Cell function in MTAP Deficient Tumors,” Cancer Cell 41:1774-1787 (2023) PMID: 37774699 DOI: 10.1016/j.ccell.2023.09.005

Wilder, C.S., Chiou, J., Battenhouse, A., Saha, A., Chen, Z., Kim, E., Gadallah, M.I., Tiziani, S., Georgiou, G., Stone, E., and J. DiGiovanni, “Enzymatic Depletion of L-Met Using an Engineered Human Enzyme as a Novel Therapeutic Strategy for Melanoma,” Mol Carcinogenesis (2023) PMID: 37378415 doi: 10.1002/mc.23597.

Saha, A., Zhao, S., Kindall, A., Wilder, C., Friedman, C.A., Clark, R., Georgiou, G., Stone, E., Kidane, D., and J. DiGiovanni, “Cysteine Depletion Sensitizes Prostate Cancer Cells to Agents that Enhance DNA Damage and to Immune Checkpoint Inhibition,” J. Exp. Clin. Cancer Res. 42:119 (2023) PMID: 37170264 doi: 10.1186/s13046-023-02677-2.

2022

Blazeck, J.,  Karamitros, S.K,  Ford, K., Somody, C., Qerqez, A., Murray, K., Burkholder, N.T., Marshall, N., Sivakumar, A., Lu, W-C., Tan, B., Lamb, C., Tanno, Y., Siddiqui, M.N., Ashoura, N., Coma, S., Zhang, X., McGovern, K., Kumada, Y. Zhang, Y.J., Manfredi, M., Johnson, K.A., S., D’Arcy, Stone, E. and G. Georgiou, “Bypassing Evolutionary Dead-ends and Switching the Rate-Limiting Step of a Human Immunotherapeutic Enzyme,” Nature Catalysis 5:952-967 (2022) PMID: 36465553 doi: 10.1038/s41929-022-00856-6.

Kuraoka, M., Curtis, N.C., Watanabe, A., Tanno, H., Shin, S., Ye, K., Macdonald, E., Lavidor, O., Kong, S., Von Holle, T., Windsor, I., Ippolito,G.C., Georgiou, G., Walter, E.B., Kelsoe, G., Harrison, S.C., Moody, M.A., Bajic, G. and J. Lee, “Infant Antibody Repertoires during the First Two Years of Influenza Vaccination,” mBio 13:e0254622 (2022) PMID: 36314798 doi: 10.1128/mbio.02546-22.

Zhang, L., Hobeika, C.S., Khabibullin, D., Yu, D., Filippakis, H., Alchoueiry, M., Tang, Y., Lam, H.C., Tsvetkov, P., Georgiou, G., Lamb, C., Stone, E., Puigserver, P., Priolo, C. and E.P. Henske, “Hypersensitivity to ferroptosis in chromophobe RCC is mediated by a glutathione metabolic dependency and cystine import via solute carrier family 7 member 11” Proc Natl Acad Sci U S A 119:e2122840119 (2022) PMID: 35867762 doi: 10.1073/pnas.2122840119

Karamitros, C.S., Murray, K., Winemiller, B., Lamb, C., Stone, E.M., D’Arcy, S., Johnson, K.A., and G. Georgiou, “Leveraging Intrinsic Flexibility to Engineer Enhanced Enzyme Catalytic Activity,” Proc. Natl. Acad. Sci. USA 119:e2118979119 (2022) PMID: 35658075 doi: 10.1073/pnas.2118979119

Delidakis, G., Kim, J.E., George, K. and G. Georgiou “Improving Antibody Therapeutics byManipulating the Fc Domain: Immunological and Structural Considerations” Ann. Rev. Biomed. Engin. 6:249-74 (2022) PMID: 35363537 doi: 10.1146/annurev-bioeng-082721-024500

Pataskar, A., Champagne, J., Nagel, R., Kenski, J., Laos, M., Michaux, J., Pak, H.S., Bleijerveld, O.B., Mordente, K., Navarro, J.M., Blommaert, N., Nielsen, N.M., Lovecchio, D., Stone, E., Georgiou G., de Gooijer, M.C., van Tellingen, O., Altelaar, M., Joosten, P.R., Perrakis, A., Olweus, J., Bassani-Sternberg, M., Peeper, D.M. and R. Agami, “Tryptophan Depletion Results in Tryptophan-to-Phenylalanine Substituants.” Nature 603:721-727 (2022) PMID: 35264796 doi: 10.1038/s41586-022-04499-2.

2021


Voss WN, Hou YJ, Johnson NV, Delidakis G, Kim JE, Javanmardi K, Horton AP, Bartzoka F, Paresi CJ, Tanno Y, Chou C-W, Abbasi SA, Pickens W, George K, Boutz DR, Towers DM, McDaniel JR, Billick D, Goike J, Rowe L, Batra D, Pohl J, Lee J, Gangappa S, Sambhara S, Gadush M, Wang N, Person MD, Iverson BL, Gollihar JD, Dye J, Herbert A, Finkelstein IJ, Baric RS, McLellan JS, Georgiou G, Lavinder JJ*, Ippolito GC*. Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes. Science (2021) 372(6546):1108-1112. doi: 10.1126/science.abg5268. PMID: 33947773.

McCarthy, K.R., Lee, J. Watanabe, A., Kuraoka, M., Robinson-McCarthy, L-R., Georgiou, G., Kelsoe, G., and Stephen C. Harrison,  “A Prevalent Focused Human Antibody Response to the Influenza Hemagglutinin Head Interface,” mBio (2021) in press

Jung J, Mundle ST, Ustyugova IV, Horton AP, Boutz DR, Pougatcheva S, Prabakaran P, McDaniel JR, King GR, Park D, Person MD, Ye C, Tan B, Tanno Y, Kim JE, Curtis NC, DiNapoli J, Delagrave S, Ross TM, Ippolito GC, Kleanthous H, Lee J, Georgiou G. Influenza vaccination in the elderly boosts antibodies against conserved viral proteins and egg-produced glycans. J Clin Invest (2021) 131(13):e148763. doi: 10.1172/JCI148763. PMID: 34196304; PMCID: PMC8245176.

Li J, Bazzi SA, Schmitz F, Tanno H, McDaniel JR, Lee CH, Joshi C, Kim JE, Monson N, Greenberg BM, Hedfalk K, Melamed E*, Ippolito GC*. Molecular Level Characterization of Circulating Aquaporin-4 Antibodies in Neuromyelitis Optica Spectrum Disorder. Neurol Neuroimmunol Neuroinflamm (2021) 8(5):e1034. doi: 10.1212/NXI.0000000000001034. PMID: 34168058; PMCID: PMC8225010.


2020


Mehaffey M.R., Lee J., Jung J., Lanzillotti M.B., Escobar E.E., Morgenstern K.R., Georgiou G., Brodbelt J.S., “Mapping a Conformational Epitope of Hemagglutinin A Using Native Mass Spectrometry and Ultraviolet Photodissociation,” Anal Chem 92(17):11869-11878 (2020) PMID: 32867493 DOI: 10.1021/acs.analchem.0c02237.

Verardi, R., Lindesmith, L.C., Tsybovsky, Y., Gorman, J., Chuang, Y.-G., Edwards, K., Jensen, P.B., Mallory, M.L., Ou, L., Schön, A., Tully, E.S. Georgiou, G. Baric, R.S. and P.D. Kwong “Disulfide-Stabilization of Human Norovirus GI.1 Virus-like Particles Focuses Immune Response Toward Neutralizing Epitopes” NPJ Vaccines (2020) in press.

Lyu, A., Triplett, T.A., Nam, S.H., Hu, Z., Arasappan, D., Godfrey, H.D., Ames, R.Y., Sarang, A., Selden, H.J., Lee, C-H., Georgiou, G., Horton, T.M., Ehrlich, L.I.R. “Tumor-Associated Myeloid Cells Provide Critical Support for T-ALL”. Blood DOI: 10.1182/blood.2020007145 PMID: 32845007

Soni, C., Perez, O.A., Voss, W.N., Pucella, J.N., Serpas, L., Mehl, J., Ching, K.L., Goike, J., Georgiou, G.,
Ippolito, G.C., Sisirak, V. and Reizis, B. “Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA” Immunity 52:1022-1038 (2020) PMID: 32454024 DOI:10.1016/j.immuni.2020.04.015

Lu, W-C., Saha, A., Yan, W., Garrison, K., Lamb, C., Pandey, R., Irani, S., Lodi, A., Lu, X., Tiziani, S., Zhang., Y.J., Georgiou, G., DiGiovanni, J. and E. Stone, “Enzyme-Mediated Depletion of Serum L-Met Abrogates Prostate Cancer Growth via Multiple Mechanisms without Evidence of Systemic Toxicity, “Proc. Natl. Acad. Sci. USA 117:532-540 (2020) PMID: 32434918, DOI: 10.1073/pnas.1917362117

Badgley, M.A., Kremer, D., Maurer, H.C., DelGiorno, K.E., Lee, H-J., Purohit, H., Sagalovskiy, I., Ma, A., Kapillian, J., Firl, E.M., Decker, A.R., Sastra, S.A, Palermo, C.F., Andrade, L.R., Sajjakulnukit, P., Zhang, L., Tolstyka, Z.P., Hirschhorn, T., Lamb, C., Liu, T., Gu, W., Seeley, E.S., Stone, E., Georgiou, G., Manor, U., Iuga, A., Wahl, G. F., Stockwell, B.R., Lyssiotis, C.A. and K. P. Olive, “Cysteine depletion induces pancreatic tumor ferroptosis in mice, “ Science 368:85-89 (2020) PMID: 32241947, DOI: 10.1126/science.aaw9872

Tanno, H., McDaniel, J.R., Stevens, C.A., Voss, W.N., Li. J., Durrett, R., Lee, J., Gollihar, J., Tanno, Y., Delidakis, G., Pothukuchy, A. Ellefson, J.W., Goronzy, J.J., Maynard, J.A., Ellington, A.D., Ippolito, G.C. and G. Georgiou, “A Facile Technology for the High Throughput Sequencing of the Paired VH:VL and TCR:TCR Repertoires ,” Science Advances 6:eaay9093 (2020) PMID: 32426460, DOI: 10.1126/sciadv.aay9093.

Tanno, H., Gould, T.M., McDaniel, J.R., Cao,W., Tanno, Y., Durrett, R.E., Park, D., Cate, S.J., Hildebrand, W.H., Dekker, C.L., Tian, L., Weyand, C.M., Georgiou, G. and J.J., Goronzy, “Determinants Governing T cell Receptor α/β-Chain Pairing in Repertoire Formation of Identical Twins,” Proc. Natl. Acad. Sci. USA 117:532-540 (2020) PMID: 31879353, DOI: 10.1073/pnas.1915008117

Lee, J., Der, B.S., Karamitros, C.S., Li, W., Marshall, N.M., Lungu, O.A., Miklos , A.E., Xu , J., Kang, T-H., Lee, C-H., Tan, B., Hughes , R.A., Jung, S-T., Ippolito , G.C.. Gray, J.J., Zhang, Y., Kuhlman, B., Georgiou G. and A.D. Ellington, “Computer-Based Engineering of Thermostabilized Antibody Fragments,” AICHE J., 66: e16864 (2020) DOI: 10.1002/aic.16864

Sterrett S, Peng BJ, Burton RL, LaFon DC, Westfall AO, Singh S, Pride M, Anderson AS, Ippolito GC, Schroeder HW Jr, Nahm MH, Krishna Prasad A, Goepfert P, Bansal A. Peripheral CD4 T follicular cells induced by a conjugated pneumococcal vaccine correlate with enhanced opsonophagocytic antibody responses in younger individuals. Vaccine. 2020 Feb 11;38(7):1778-1786. doi: 10.1016/j.vaccine.2019.12.023. Epub 2020 Jan 3. PMID: 31911030.


2019


Lee CH, Kang TH, Godon O, Watanabe M, Delidakis G, Gillis CM, Sterlin D, Hardy D, Cogné M, Macdonald LE, Murphy AJ, Tu N, Lee J, McDaniel JR, Meyer AS, Bruhns P, Georgiou G. An engineered human Fc domain that behaves like a pH-toggle switch for ultra-long circulation persistence. Nature Communications. 2019 Nov 6;10(1):1-1. PMID: 31695028 DOI: 10.1038/s41467-019-13108-2.
.
Lang X, Green MD, Wang W, Yu J, Choi JE, Jiang L, Liao P, Zhou J, Zhang Q, Dow A, Saripalli AL. Radiotherapy and immunotherapy promote tumoral lipid oxidation and ferroptosis via synergistic repression of SLC7A11. Cancer discovery. 2019 Dec 1;9(12):1673-85. PMID:31554642 DOI: 10.1158/2159-8290.CD-19-0338.

Dekker JD, Baracho GV, Zhu Z, Ippolito GC, Schmitz RJ, Rickert RC, Tucker HO. Loss of the FOXP1 Transcription Factor Leads to Deregulation of B Lymphocyte Development and Function at Multiple Stages. Immunohorizons. 2019 Oct 7;3(10):447-462. PMID: 31591252. DOI: 10.4049/immunohorizons.1800079.

Taft, J.M., Georgeon, S., Allen, C., Reckel, S., DeSautelle, J., Hantschel, O., Georgiou, G., Iverson, B.L., “Rapid Screen for Tyrosine Kinase Inhibitor Resistance Mutations and Substrate Specificity,” ACS Chem Biol. 14(9):1888-1895 (2019) PMID: 31339688, DOI: 10.1021/acschembio.9b00283.

Kshattry, S., Saha, A., Gries, P., Tiziani, S., Stone, EM., Georgiou, G., DiGiovanni, J., “Enzyme-mediated depletion of l-cyst(e)ine synergizes with thioredoxin reductase inhibition for suppression of pancreatic tumor growth,” NPJ Precis Oncol. (2019) eCollection 2019, PMID: 31231686. DOI: 10.1038/s41698-019-0088-z.

Lindesmith, L.C., McDaniel, J.R., Changela, A., Varardi, R., Kerr S.A., Constantini, V., Brewer-Jensen, P.D., Mallory, M.L., Voss, W.N., Boutz, D.R., Blazeck, J. J., Ippolito, G.C., Vinje, J., Kwong, P.D., Georgiou, G. and Baric, R. “Boosted Broad Binding/Blockade Antibodies Dominate the Serological Repertoire to Norovirus Vaccine” Immunity 50: 1530-1541(2019) PMID: 31216462 DOI: 10.1016/j.immuni.2019.05.007

Wang, W., Green, M., Choi, J.E., Gijón, M., Kennedy, P.D., Johnson, J.K., Liao, P., Lang, X., Kryczek, I., Sell, A., Xia, H., Zhou, J., Li, G., Li, J., Li, W., Wei, S., Vatan, L., Zhang, H., Szeliga, W., Gu, W., Liu, R., Lawrence, T.S., Lamb, C., Tanno, Y., Cieslik, M., Stone, E., Georgiou. G., Chan, T.A., Chinnaiyan, A. and W. Zou, “CD8+ T Cells Regulate Tumour Ferroptosis During Cancer Immunotherapy” Nature (2019) PMID: 31043744 DOI: 10.1038/s41586-019-1170-y

Kang, T.H., Lee, C.H., Delidakis, G., Jung, J., Richard-Le Goff, O., Lee, J., Kim, J.E., Charab, W., Bruhns, P. and G. Georgiou, “An Engineered Human Fc variant With Exquisite Selectivity for FcγRIIIaV158 Reveals That Ligation of FcγRIIIa Mediates Potent Antibody Dependent Cellular Phagocytosis With GM-CSF-Differentiated Macrophages,” Front Immunology 10:562 (2019) PMID: 30984171 DOI: 10.3389/fimmu.2019.00562

Krebs, S.J., Kwon, Y.D., Schramm, C.A., Law, W.H., Donofrio, G., Zhou, K.H., Gift, S., Dussupt, V., Georgiev, I.S., Schätzle, S., McDaniel, J.R., Lai, Y.T., Sastry, M., Zhang, B., Jarosinski, M.C., Ransier, A., Chenine, A.L., Asokan, M., Bailer, R.T., Bose, M., Cagigi, A., Cale, E.M., Chuang, G.Y., Darko, S., Driscoll, J.I., Druz, A., Gorman, J., Laboune, F., Louder, M.K., McKee, K., Mendez, L., Moody, M.A., O’Sullivan, A.M., Owen, C., Peng, D., Rawi, R., Sanders-Buell, E., Shen, C.H., Shiakolas, A.R., Stephens, T., Tsybovsky, Y., Tucker, C., Verardi, R., Wang, K., Zhou, J., Zhou, T., Georgiou, G., Alam, S.M., Haynes, B.F., Rolland, M., Matyas, G.R., Polonis, V.R., McDermott, A.B., Douek, D.C., Shapiro, L., Tovanabutra, S., Michael, N.L., Mascola, J.R., Robb, M.L., Kwong, P.D. and N.A. Doria-Rose, “Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual,” Immunity 50:677-691 (2019) PMID: 30876875 DOI: 10.1016/j.immuni.2019.02.008

Lee, J., Paparoditis, P., Horton, A.P., Frühwirth, A., McDaniel, J.R., Jung, J., Boutz, D.R., Hussein, D.A., Tanno, Y., Pappas, L., Ippolito, G.C., Corti, D., Lanzavecchia, A. and G. Georgiou, “Persistent Antibody Clonotypes Dominate the Serum Response to Influenza over Multiple Years and Repeated Vaccinations,” Cell Host Microbe 25:367-376 (2019) PMID: 30795981 DOI: 10.1016/j.chom.2019.01.010

Manso, T.C., Groenner-Penna, M., Minozzo, J.C., Antunes, B.C., Ippolito, G.C., Molina, F., and Felicori, L.F., “Next-generation sequencing reveals new insights about gene usage and CDR-H3 composition in the horse antibody repertoire,” Mol. Immunology 105:251-259 (2019) PMID: 30562645

2018


Johnson, E.L., Doria-Rose, N.A., Gorman, J., Bhiman, J.N., Schramm, C.A., Vu, A.Q., Law, W.H., Zhang, B., Bekker, V., Karim, S.S.A., Ippolito, G.C., Morris, L., Moore, P.L. Kwong, P.D., Mascola, J.R. and G. Georgiou, “Sequencing HIV-neutralizing antibody exons and introns reveals detailed aspects of lineage maturation” Nature Communications 9(1):4136 (2018) PMID: 30297708 DOI:10.1038/s41467-018-06424-6

Saphire, E.O., Schendel, S.L., Fusco, M.L., Gangavarapu, K., Gunn, B.M., Wec, A.Z., Halfmann, P.J., Brannan, J.M., Herbert, A.S., Qiu, X., Wagh, K., He, S., Giorgi, E.E., Theiler J, Pommert KBJ, Krause TB, Turner HL, Murin CD, Pallesen J, Davidson E, Ahmed R, Aman MJ, Bukreyev A, Burton DR, Crowe JE Jr, Davis CW, Georgiou G, Krammer F, Kyratsous CA, Lai JR, Nykiforuk C, Pauly MH, Rijal P, Takada A, Townsend AR, Volchkov V, Walker LM, Wang CI, Zeitlin L, Doranz BJ, Ward AB, Korber B, Kobinger GP, Andersen KG, Kawaoka Y, Alter G, Chandran K, Dye JM; Viral Hemorrhagic Fever Immunotherapeutic Consortium, “Systematic Analysis of Monoclonal Antibodies against Ebola Virus GP Defines Features that Contribute to Protection,” Cell 2018 Aug 9;174(4):938-952.e13. doi: 10.1016/j.cell.2018.07.033. PMID: 30096313

Triplett, T.A., Garrison, K.C., Marshall, N., Donkor, M., Blazeck, J., Lamb, C., Qerqez A., Dekker, J.D., Tanno, Y. Lu, W-C., Karamitros, S.K., Ford, K., Tan, B., Zhang, M., McGovern, K., Coma, S., Kumada, Y., Yamany, M.S., Sentandreu, S., Fromm, G., Tiziani, S., Schreiber, T.H., Manfredi, M., Ehrlich, L.I.R., Stone E., and G. Georgiou, “Reversal of IDO-Mediated Cancer Immune Suppression by Systemic Kynurenine Depletion with a Therapeutic Enzyme,” Nature Biotechnology doi: 10.1038/nbt.4180

Lee, J. and G. Georgiou, “High Affinity IgA Against Microbial Antigens” Nature Immunology 2018 19:514-515 (2018) PMID: 29777208

Liu, Y., McDaniel, J.R., Khan, S., Campisi, P., Propst, E.J., Holler, T., Grunebaum, E., Georgiou, G., Ippolito, G.C. and Ehrhardt, G.R.A., “Antibodies Encoded by FCRL4-Bearing Memory B Cells Preferentially Recognize Commensal Microbial Antigens,” J Immunology 200:3962-3969 (2018) PMID: 29703863

Chen, Y.Q., Wohlbold, T.J., Zheng, N.Y., Huang, M., Huang, Y., Neu, K.E., Lee, J., Wan, H., Rojas. K.T., Kirkpatrick., E., Henry, C., Palm, A.E., Stamper, C.T., Lan, L.Y., Topham, D.J., Treanor, J., Wrammert, J., Ahmed, R., Eichelberger, M.C., Georgiou, G, Krammer, F. and Wilson, P.C., “Influenza Infection in Humans Induces Broadly Cross-Reactive and Protective Neuraminidase-Reactive Antibodies,” Cell 173:417-429 (2018). PMID: 29625056

  • Commentaries and News Stories: Covered by 9 scientific and mainstream media publications

McDaniel, J.R., Pero, S.C., Voss, W.N., Shukla, G.S., Sun, Y., Schaetzle, S., Lee, C.H., Horton, A.P., Harlow, S., Gollihar, J., Ellefson, J.W., Krag, C.C., Tanno, Y., Sidiropoulos, N., Georgiou, G., Ippolito, G.C. and Krag, D.N., “Identification of Tumor-Reactive B Cells and Systemic IgG in Breast Cancer Based on Clonal Frequency in the Sentinel Lymph Node,” Cancer Immunology Immunother 67:729-738 (2018) PMID: 29427082

Wang, B., DeKosky, B.J., Timm, M.R., Lee, J., Normandin, E., Misasi, J., Kong, R., McDaniel, J.R., Delidakis, G., Leigh, K.E., Niezold, T., Choi, C.W., Viox, E.G., Fahad, A., Cagigi, A., Ploquin, A., Leung, K., Yang, E.S., Kong, W.P., Voss, W.N., Schmidt, A.G., Moody, M.A., Ambrozak, D.R., Henry, A.R., Laboune, F., Ledgerwood, J.E., Graham, B.S., Connors, M., Douek, D.C., Sullivan, N.J., Ellington, A.D., Mascola, J.R. and G. Georgiou, “Functional Interrogation and Mining of Natively Paired Human VH:VL Antibody Repertoires,” Nature Biotechnology 36:152-155 (2018) PMID: 29309060



2017


McDaniel, J.R., Ippolito G.C. and G. Georgiou, “Mapping the Secrets of the Antibody Pool,” Nature Biotechnology 35:921-922. (2017) PMID: 28892077.

Jang, S-H., Gregersen, P.K., Georgiou, G., Diamond, B., “Increased Cathepsin S in Dendritic Cells Alters TFH Repertoire and Contributes to Lupus,”, Nature Immunology 18:1016-1024 (2017) PMID: 28692065

Cotham, V.C., Horton, A.P., Lee, J., Georgiou, G. and Brodbelt, J.S., “Middle-down 193-nm Ultraviolet Photodissociation for Unambiguous Antibody Identification and its Implications for Immunoproteomic Analysis,” Anal. Chem. 89:6498-6504 (2017) PMID: 28517930

Lee, C.-H., Romain, G., Yan, W., Watanabe, W., Charab, W., Todorova, B., Lee, Y., Triplett, K., Donkor, M., Lungu, O.I., Lux, A., Marshall, N., Lindorfer, M.A., Richard-Le Goff, O., Balbino, B., Kang, T.H., Tanno, H., Delidakis, G., Alford, C., Taylor, R.P., Nimmerjahn, F., Varadarajan, N., Bruhns, P. Zhang, Y.J.  and G. Georgiou, “IgG Fc Domains that Bind C1q but not Effector Fcγ Receptors Delineate the Significance of Complement-Dependent Cell-Mediated Cytotoxicity and Phagocytosis in Antibody Function,” Nature Immunology 18:889-898 (2017) DOI: 10.1038/ni.3770

Lau, D., Lan, L.Y., Andrews, S.F., Henry, C., Thatcher Rojas, K., Neu, K.E., Huang, Y., DeKosky, B., Palm, A.E., Ippolito, G.C., Georgiou, G., Wilson, P., “Low CD21 expression defines a population of recent germinal center graduates primed for plasma cell differentiation” Science Immunology Vol. 2, Issue 7 (2017) DOI: 10.1126/sciimmunol.aai8153

Steiniger, S.C., Glanville, J., Harris, D.W., Wilson, T.L., Ippolito, G.C., and Dunham, S.A., “Comparative analysis of the feline immunoglobulin repertoire,” Biologicals 46:81-87 (2017) PMID: 28131552

Williams, L.D., Ofek, G., Schatzle, S., McDaniel, J.R., Lu, X., Nicely, N.I., Wu, L., Lougheed, C.S., Bradley, T., Louder, M.K., McKee, K., Bailer, R.T., O’Dell, S., Georgiev, I.S., Seaman, M., Parks, R., Marshall, D., Anasti, K., Yang, G., Nie, X., Tumab, N., Wiehe, K., Wagh, K., Korber, B., Kepler, T., Alam, S.M., Morris, L., Kamanga, G., Choen, M.S., Bonsignori, M., Xia, S., Montefiori, D., Kelsoe, G., Gao, F., Mascola, J.R., Moody, M.A., Saunders, K.O., Liao, H., Tomaras, G.D., Georgiou, G., Haynes, B.F., “Potent and broad HIV-neutralizing antibodies in memory B cells and plasma”, Science Immunology 27 Jan 2017: Vol. 2, Issue 7. DOI: 10.1126/sciimmunol.aal2200

Li, Q., Yi, L., Hoi, K.H., Marek, P., Georgiou, G. and Iverson, B.L., “Profiling Protease Specificity: Combining Yeast ER Sequestration Screening (YESS) with Next Generation Sequencing,” ACS Chem. Biol. (2017) PMID: 27977123

Cramer, S.L., Saha, A., Liu, J., Tadi, S., Tiziani, S., Yan, W., Triplett, K., Lamb, C., Alters, S.E., Rowlinson, S., Zhang, Y., Keating, M.J., Huang, P., DiGiovanni, J., Georgiou, G., and E. Stone, “Systemic Depletion of Serum L-Cyst(e)ine with an Engineering Human Enzyme Mediates Potent Induction of ROS Leading to Selective Cancer Ablation,” Nature Medicine 23, 120-127, doi: 10.1038/nm.4232 (2017) PMID: 27869804

  • Commentaries and News Stories: Covered by >24 scientific and mainstream media publications; e.g. Cancer Discovery: http://cancerdiscovery.aacrjournals.org/content/early/2016/12/15/2159-8290.CD-RW2016-235 ; Yahoo Finance: http://finance.yahoo.com/news/study-published-nature-medicine-highlights-122000477.html

2016


Wu, G.C., Cheung, N.V., Georgiou, G., Marcotte, E.M., Ippolito, G.C., “Temporal Stability and Molecular Persistence of the Bone Marrow Plasma Cell Antibody Repertoire,” Nat Commun. 7:13838 (2016) PMID: 28000661. DOI: 10.1038/ncomms13838

Lee, J., Boutz, D.R., Chromikova, V., Joyce, M.G., Vollmers, C., Leung, K., Horton, A.P., DeKosky, B.J., Lee, C.H., Lavinder, J.J., Murrin, E.M., Chrysostomou, C., Hoi, K.H., Tsybovsky, Y., Thomas, P.T., Druz, A., Zhang, B., Zhang, Y., Wang, L., Kong, W.P., Park, D., Popova, L.I., Dekker, C.L., Davis, M.M., Carter, C.E., Ross, T.M., Ellington, A.D., Wilson, P.C., Marcotte, E.M., Mascola, J.R., Ippolito, G.C., Krammer, F., Quake, S.R., Kwong, P.D. and Georgiou, G., “Molecular-Level Analysis of the Serum Antibody Repertoire in Young Adults Before and After Seasonal Influenza Vaccination,” Nature Medicine 22(12):1456-1464 (2016) PMID: 27820605. DOI: 10.1038/nm.4224

  • Commentaries and News Stories: Covered by >20 scientific and mainstream media publications;

e.g. Drug Discovery & Development: https://www.dddmag.com/news/2016/11/flu-vaccines-effectiveness-could-be-improved-new-findings-suggest

Raymond, D., Stewart, S., Lee, J., Ferdman, J., Bajic, G., Do, K.T., Ernandes, M.J., Suphaphiphat, P., Settembre, E.C., Dormitzer, P.R., Del Giudice, G., Finco, O., Kang, T.H., Ippolito, G.C., Georgiou, G., Kepler, T.M., Haynes, B.F., Moody, M.A., Liao, H-X., Schmidt, A.G. and  S.C. Harrison, “Influenza Immunization Elicits Antibodies Specific for an Egg-Adapted Vaccine Strain,” Nature Medicine 22(12):1465-1469 (2016) PMID: 27820604. DOI: 10.1038/nm.4223

Wang, B., Lee, C.H., Johnson, E.L., Kluwe, C.A., Cunningham, J.C., Tanno, H., Crooks, R.M., Georgiou. G. and A.D. Ellington, “Discovery of High Affinity Anti-Ricin Antibodies by B Cell Receptor Sequencing and by Yeast Display of Combinatorial VH:VL Libraries from Immunized Animals, “MAbs. 8(6):1035-1044. (2016) PMID: 27224530. DOI: 10.1080/19420862.2016.1190059DeKosky, B.J., Lungu, O., Park, D., Johnson, E.L, Charab, W., Chrysostomou, C., Kuroda, D., Ellington, A.D., Ippolito, G.C., Gray, J.J. and G. Georgiou, “Large-Scale Sequence and Structural Comparisons of Human Naïve and Antigen-Experienced Antibody Repertoires,”  Proc. Natl. Acad. Sci. USA 113(19):E2636-45 (2016) PMID: 27114511. DOI: 10.1073/pnas.1525510113

Biswas, P., Chavali, V.R., Agnello, G., Stone, E., Chakarova, C., Duncan, J.L., Kannabiran, C., Homsher, M., Bhattacharya, S.S., Naeem, M.A., Kimchi, A., Sharon, A., Iwata, T., Riazuddin, S., Reddy, G.B., Hejtmancik, J.F., Georgiou, G., Riazuddin, S.A. and R. Ayyagari, “A Missense Mutation in ASRGL1 is Involved in Causing Autosomal Recessive Retinal Degeneration” Hum Mol Genet. 25(12):2483-2497 (2016) PMID: 27106100. DOI: 10.1093/hmg/ddw113

Brezski, R.J. and G. Georgiou, “Immunoglobulin Isotype Knowledge and Application to Fc Engineering,” Curr. Opin. Immunol. 40:62-69 (2016). PMID: 27003675. DOI: 10.1016/j.coi.2016.03.002

Dekker, J.D., Park, D., Shaffer, A.L., Kohlhammerc, H., Deng, W., Lee, B.-K., Ippolito, C.C., Georgiou, G.,Iyer, V.R., Staudt, L.M. and H.O. Tucker, “Subtype-Specific Addiction of the Activated B-Cell Subset of Diffuse Large B Cell Lymphoma to FOXP1,” Proc. Natl. Acad. Sci. USA 113:E577-86 (2016). PMID: 26787899. DOI: 10.1073/pnas.1524677113

McDaniel, J.R., DeKosky, B., Tanno, H., Ellington, A.D. and G. Georgiou, “Ultra- high-throughput Sequencing of the Immune Receptor Repertoire from Millions of Lymphocytes,” Nature Protocols 11:429-442 (2016) PMID: 26844430. DOI: 10.1038/nprot.2016.024

Murzabaev, M., Kojima, T., Mizoguchi, T., Kobayashi, I., DeKosky, B.J., Georgiou, G., H. Nakano, “Handmade Microfluidic Device for Biochemical Applications in Emulsion,” J Biosci Bioeng. 121(4):471-476 (2016) PMID: 26386750. DOI: 10.1016/j.jbiosc.2015.08.001

Gorman, J., Soto, C., Yang, M.M., Davenport, T.M, Guttman, M., Bailer, R.T., Chambers, M., Chuang, G.Y., DeKosky, B.J., Doria-Rose, N.A., Druz, A., Ernandes, M.J., Georgiev, I.S., Jarosinski, M.C., Joyce, M.G., Lemmin, T.M., Leung, S., Louder, M.K., McDaniel, J.R., Narpala, S., Pancera, M., Stuckey, J., Wu, X., Yang, Y., Zhang, B., Zhou, T., Program, N.C., Mullikin, J.C., Baxa, U., Georgiou, G., McDermott, A.B., Bonsignori, M., Haynes, B.F., Moore, P.L., Morris, L., Lee, K.K., Shapiro, L., Mascola, J.R., Kwong, P.D., “Structures of HIV-1 Env V1V2 with Broadly Neutralizing Antibodies Reveal Commonalities that Enable Vaccine Design,” Nat Struct. Mol Biol.  23(1):81-90 (2016).  PMID: 26689967. DOI: 10.1038/nsmb.3144

2015


Quandt, E., Gollihar, J., Blount, Z., Ellington, A.D., Georgiou, G. and J.E Barrick, “Fine-Tuning Citrate Synthase Flux Potentiates and Refines Metabolic Innovation in the Lenski Evolution Experiment,” Elife doi: 10.7554/eLife.09696 (2015) PMID: 26465114.

Burrage, L.C., Sun, Q., Elsea, S.H., Jiang, M.N., Nagamani, S.C., Frankel, A.E., Stone, E., Alters, S.E., Johnson, D.E., Rowlinson, S.W., Georgiou, G, Members of the Urea Cycle Disorders Consortium and B.H. Lee, “Human Recombinant Arginase Enzyme Reduces Plasma Arginine in Mouse Models of Arginase Deficiency,” Hum Mol Genet. 24:6,417-27 (2015). PMID: 26358771

Kerr, S.A., Jackson, E.L., Lungu, O.I., Meyer, A.G., Demogines, A., Ellington, A.D., Georgiou, G., Wilke, C.O. and S. L. Sawyer, “Computational and Functional Analysis of the Virus-Receptor Interface Reveals Host Range Trade-offs in New World Arenaviruses,” J. Virol. 89:11643-53 (2015) PMID: 26355089.

Wang, B., Kluwe, C.A., Lungu, O.I., DeKosky, B.J., Kerr, S.A., Johnson, E.L., Jung, J., Rezigh, A.B., Carroll1, S.M., Reyes, A.N., Bentz, J.R., Villanueva, I., Altman, A.L., Davey, R.A., Ellington, A.D. and G. Georgiou, “Facile Discovery of a Diverse Panel of Anti-Ebola Virus Antibodies by Immune Repertoire Mining,” Scientific Reports 5:13926. doi: 10.1038/srep13926 (2015) PMID: 26355042.

Wine, Y., Horton, A.P., Ippolito, G.C. and Georgiou G., “Serology in the 21st Century: the Molecular-Level Analysis of the Serum Antibody Repertoire,” Curr Opin Immunol. 35:89-97 (2015) PMID: 26172290

Yi, L., Taft, J.M., Li, Q., Gebhard, M.C., Georgiou, G. and Iverson, B.L., “Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast” Methods Mol Biol. 1319:81-93 (2015) PMID: 26060071

Chrysostomou, C., Quandt, E. M., Marshall, N.M., Stone, E. and Georgiou G.  “An Alternate Pathway of Arsenate Resistance in E. coli Mediated by the Glutathione-S-Transferase GstB,” ACS Chem Bio (2015) PMID: 25517993

DeKosky, B.J., Kojima, T., Rodin, A., Charab, W., Ippolito, G.C., Ellington, A.D. and G. Georgiou, “Ultra-High Throughput Antibody VH:VL Sequencing Using a Novel Single-Cell Flow Focusing Technology,” Nature Medicine 21:86-91(2015) PMID: 25501908

Lavinder, J.J., Horton, A.P., Georgiou, G and Ippolito G.C., “Next-Generation Sequencing and Protein Mass Spectrometry for the Comprehensive Analysis of Human Cellular and Serum Antibody Repertoires,” Curr Opin Chem Biol.  24:112-120 (2015) PMID: 25461729

2014


Kelton, W., Mehta, N., Charab, W., Lee, J., Lee, C.H., Kojima, T., Kang, T.-H. and G. Georgiou, “IgGA: A “Cross-Isotype” Engineered Human Fc Antibody Domain that Displays Both IgG-like and IgA-like Effector Functions,”  Chem & Biol. 21(12):1603-9 (2014) PMID: 25500223. DOI: 10.1016/j.chembiol.2014.10.017

  • Chem & Bio featured Article

Romain G., Senyukov, V., Rey-Villamizar, N., Merouane, A., Kelton, W., Liadi, I., Mahendra, A., Charab, W., Georgiou, G., Roysam, B., Lee, D.A. and N. Varadarajan, “Antibody Fc-engineering Improves Frequency and Promotes Kinetic Boosting of Serial Killing Mediated by NK Cells,” Blood 124(22):3241-9 (2014) PMID: 25232058. DOI: 10.1182/blood-2014-04-569061

Lavinder, J.J., Hoi, K.H., Reddy, S.T., Wine, Y. and G. Georgiou, “Systematic Characterization and Comparative Analysis of the Rabbit Immunoglobulin Repertoire”, PLoS One 9(6):e101322 (2014) PMID: 24978027. DOI: 10.1371/journal.pone.0101322

Boutz, D.R., Horton, A.P., Wine, Y., Lavinder, J., Georgiou, G. and E.M. Marcotte,” “Proteomic Identification of Monoclonal Antibodies from Serum, Anal. Chem. 86:4758-66 (2014) PMID: 24684310. DOI: 10.1021/ac4037679

 Mroczek, E.S., Ippolito, G.C., Rogosch, T., Hoi, K.H., Hwangpo, T.A., Brand, M.G., Zhuang, Y., Liu, C.R., Schneider, D.A., Zemlin, M., Brown, E.E., Georgiou, G., Schroeder, HW Jr. “Differences in the Composition of the Human Antibody Repertoire by B Cell Subsets in the Blood,” Front. Immunol. 5:96 (2014) PMID: 24678310. DOI: 10.3389/fimmu.2014.00096

Laserson, U., Vigneault, F., Gadala-Maria, D., Yaari, G., Uduman, M., Vander Heiden, J.A., Kelton, W., Jung, S.-T., Liu, Y.-P., Lasserson, J., Chari, R., Lee, J., Bachelet, I., Hickey, B., Lieberman-Aiden, E., Hanczaruk, B., Simen, B.B., Egholm, M., Koller, D., Georgiou, G., Kleinstein, S., George Church, “High-Resolution Antibody Dynamics of Vaccine-Induced Immune Responses” Proc. Natl. Acad. Sci. USA 111(13):4928-4933 (2014) PMID: 24639495. DOI: 10.1073/pnas.1323862111

Doria-Rose, N.A., Schramm, C.A., Gorman, J., Moore, P.L., Bhiman, J.N., DeKosky, B., Ernandes, M.J., Georgiev, I.S., Kim, H.J., Pancera, M., Staupe, R.P., Altae-Tran, H.R., Bailer, R.T., Crooks, E.T., Cupo, A., Druz, A., Garrett, J.N., Hoi, K.H., Kong, R., Louder, M.K., Longo, N.S., McKee, K., Nonyane, M., O’Dell, S., Roark, R.S., Rudicell, R.S., Schmidt, S.D., Sheward, D.J., Soto, C., Wibmer, C.K., Yang, Y. Zhang, Z., Mullikin, J.C., Binley, J.M., Sanders, R.W., Wilson, I.A., Moore, J.P., Ward, A.B., Georgiou, G., Williamson, C., Abdool Karim, S.S., Morris, L., Kwong, P.D., Shapiro, L., Mascola, J.R., “Developmental Pathway for Potent V1V2-Directed HIV-1-Neutralizing Antibodies,” Nature 509:55-62 (2014) PMID: 24590074

Georgiou G., Ippolito, G., Beuasang, G., Busse, C.E., Wardemann, H. and S.R. Quake, “The Promise and Challenge of High-throughput Sequencing of the Antibody Repertoire,” Nature Biotechnol. 32:158-68 (2014) PMID: 24441474.

Quandt, E.M., Deatherage, D.E., Ellington, A.D., Georgiou, G. and J. E. Barrick, “Recursive genome-wide recombination and sequencing reveals a key refinement step in the evolution of a metabolic innovation in E. coli,” Proc. Natl. Acad. Sci. USA 111:2217-22 (2014) PMID: 24379390

National Geographic: http://phenomena.nationalgeographic.com/2014/01/06/evolution-hidden-in-plain-sight/; Proc. Natl. Acad. Sci. USA: .http://www.pnas.org/site/highlights/highlights.xhtml#trait; Proc. Natl. Acad. Sci. USA Commentary: http://www.pnas.org/content/111/6/2056.full; Le Scienze:

http://www.lescienze.it/news/2014/01/03/news/nuovo_tratto_successo_evolutivo_raffinamento-1946457/;  Technology.org: http://www.technology.org/2014/01/29/unpacking-past-identifying-key-evolutionary-step-e-coli-metabolism/ ;

Lavinder, J., Wine, Y., Giesecke, C., Ippolito, G.C., Horton, A.P., Hoi, K.-H., DeKosky, B.J., Ellington, A.D., Dörner, T., Marcotte, E.M., Boutz, D.R. and G. Georgiou, “Dynamics, Functionality and Discordance of the Human Serum Antibody and BCR Repertoires Following Immunization” Proc. Natl. Acad. Sci. USA 111:2259-64 (2014) PMID: 24469811

•  Commentaries: Highlight, Proc. Natl. Acad. Sci: http://www.pnas.org/content/111/6/2049.full

2013

Tanios, R., Bekdash, A., Kassab. E., Stone, E., Georgiou, G., Frankel, A.E., Abi-Habib, R.J. “Human Recombinant Arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-Induced Arginine Depletion is Selectively Cytotoxic to Human Acute Myeloid Leukemia Cells,” Leuk Res. 37:1565-71 (2013) PMID: 24018014.Paley, O., Agnello, G., Cantor, J., Yoo, T.H., Georgiou, G., Stone E.M., “GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria,” Methods Mol. Biol. 978:31-44 (2013) PMID: 23423887DeKosky, B.J., Ippolito, G.C., Deschner, R.P., Lavinder, J.J., Rawlings, M.P., Varadarajan, N., Wine, Y., Giesecke, C., Dörner, T., Andrews, S.F., Wilson, P.C., Hunicke-Smith, S.P., Wilson, C.P., Ellington, A.D. and G. Georgiou, “High Throughput Determination of the Antibody VH:VL Paired Repertoires,” Nature Biotechnol. 31:166-69(2013) PMID: 23334449Needham, B.D., Carroll, S., Giles, D.K., Georgiou, G., Whiteley, M., and Trent, M.S.  “Modulating the Innate Immune Response by Combinatorial Engineering of Endotoxin” Proc. Natl. Acad. Sci. USA 110:1464-69 (2013) PMID: 23297218

Wine, Y., Boutz, D., Lavinder, J.J., Horton, A., Miklos, A.E., Hughes, R.A., Jung, S.T, Murrin E.M., Ellington, A.D., Marcotte, E.M. and G. Georgiou,  “Molecular Deconvolution of the Monoclonal Antibodies that Comprise the Polyclonal Serum Response,” Proc. Natl. Acad. Sci. USA 110: 2993-8(2013) PMID: 23382245Carroll, S. and G. Georgiou,“Antibody-Mediated Inhibition of Human C1s and the Classical Complement Pathway, “Immunobiology doi:pii: S0171-2985(13)00006-5. 10.1016/j.imbio.2013.01.001 (2013).Mittal, J., Yoo, T-H., Georgiou, G. and T.M. Truskett, “Structural Ensemble of an Intrinsically Disordered Polypeptide” J Phys Chem B. 117:118-24 (2013) PMID: 23205890Jung, S. T., Kelton, W., Kang, T. H.; Ng, D., Anderssen, J. T.; Sandlie, I., Sarkar, C., G. Georgiou,
“Effective Phagocytosis of Low Her2 Tumor Cell Lines with Engineered, Aglycosylated IgG Displaying High FcγRIIa Affinity and Selectivity” ACS Chem. Bio. 8:368-75 (2013) PMID: 23030766
[/av_textblock]

2012

Saggy, I., Wine, Y., Georgiou G. and Benhar, I., “Antibody Isolation from Immunized Animals: Comparison of Phage Display and Antibody Discovery via V gene Repertoire Mining” PEDS (2012) PMID: 22988130Stone, E.M., Paley, O., Hu, J., Ekerdt, B.; Cheung, N.-K. and G., George, “De novo Engineering of a Human Methionine-γ-Lyase for Systemic L-Met Depletion Cancer Therapy,” ACS Chem. Bio. (2012) PMID: 22963240

Romero, P.A., Stone, E.M., Lamb, C., Chantranupong, L., Krause, A., Miklos, A.E., Hughes, R.A., Fechtel, B., Ellington, D.A., Arnold, F.H. and G. Georgiou, “SCHEMA-Designed Variants of Human Arginase I and II Reveal Sequence Elements Important to Stability and Catalysis”, ACS Synth. Biol. 1:221-28 (2012) PMID: 22737599Li W., Cantor, J., Yogesha, S.D.,  Yang, S., Chantranupong, L., Liu, J.Q., Agnello, G., G. Georgiou, Stone E.M.,  and Y. Zhang,“Uncoupling Intramolecular Processing and Substrate Hydrolysis in the N-terminal Hydrolase hASRGL1 by Circular Permutation” ACS Chem. Bio. Epub ahead of print (2012) PMID: 22891768Borrok, J.M., Jung, S.T., Kang, T.H. ; Monzingo, A. and G. Georgiou, Revisiting the Role of Glycosylation in the Structure of Human IgG Fc”  ACS Chem. Bio (2012) PMID: 22747430Rani, M., Bolles, M., Donaldson, E.F., Van Blarcom, T.,  Baric, R., Iverson, B.L. and G. Georgiou “Increased Antibody Affinity Confers Broad In Vitro Protection Against Escape Mutants of SARS-CoV”  J. Virology 86:9113-21 (2012)  PMID: 2269665Skretas, G.  Makino, T. Varadarajan N.,  Pogson M. and G. Georgiou,  “Multi-Copy Genes that Enhance the Yield of Mammalian G Protein-Coupled Receptors in Escherichia coli” Metabolic Eng. 14:591-602 (2012) PMID: 22609824Mauldin, J.P., Zeinali, I., Kleypas, K., Woo, J.H., Blackwood, R.S., Jo, C.H., Stone, E.M., Georgiou G., Frankel A. E.,Transl. Oncology 5:26-31 (2012) PMID: 22348173Ippolito, G.C.,  Hoi K.H., Reddy S.T., Carroll S.M., Ge X., Rogosch T., Zemlin M., Shultz L.D., Ellington A.D., Vandenberg C.L., and G. Georgiou, “Antibody Repertoires in Humanized NOD-scid-IL2Rγnull Mice
and Human B Cells Reveals Human-like Diversification and Tolerance Checkpoints in the Mouse” PLoS One7:e35497 (2012) PMID: 2255816Miklos, A.E., Kluwe, C., Der. B.S., Pai, S., Sircar, A., Hughes, R.A., Berrondo, M., Xu, J., Codrea, V., Buckley,
P.E., Calm, A.M., Walsh, H.S., Warner, C.R., Zacharko, M.A., Carney, J.P., Georgiou, G., Kuhlman, B. and A.D. Ellington,  “Structure-Based Design of Supercharged, Highly Thermostable Antibodies,”  Chem. & Biol.  19:449-55 (2012) PMID: 22520751Yoo, T-H., Pogson, M., Iverson, B.L. and G. Georgiou,  “Directed Evolution of Highly Selective Proteases Using a Novel FACS Based Screen that Capitalizes on the p53 Regulator MDM2” ChemBiochem 3:649-53(2012) PMID: 22334509Cantor,  J.R., Panayotou, V., Agnello, G., Stone. E.M. and G. Georgiou, “Engineering Reduced Immuno–genicity Enzymes for Amino Acid Depletion Therapy in Cancer,”  Methods Enzymol. 502:291-312 (2012). PMID:22208990Agrawal, V., Boyer, A.L., Woo, J-H., Mauldin,J.P., Chanhee, J. Stone, E.M., Georgiou, G. and Arthur E. Frankel, ” Cytotoxicity of Human Recombinant Arginase I [Co] PEG5000 in the Presence of L-Citruline is Dependent on Arginosuccinate Synthetase Expression in Human Cells”Anti-Cancer Drugs 23:51-64 (2012)PMID:21955999Stone, E. M., Chantrranupong L., Gonzalez, C., O’Neal, J., Rani, M., VanDenBerg, C. and G. Georgiou, “Strategies for Optimizing the Serum Persistence of Engineered Human Arginase I for Cancer Therapy,” J. Control. Release58L171-9 (2012) PMID 22001609.
[/av_textblock]

Dr. Everett Stone Competes on Forged in Fire on the History Channel

/in /by

Our very own Dr. Everett Stone recently competed on the TV show Forged in Fire on the History Channel. Congratulations Dr. Stone!

The full episode can be viewed here:

https://www.history.com/shows/forged-in-fire/season-6/episode-30

photo credit: Marsha Miller

Dr. Stone was recently featured in the Texas Scientist Magazine. https://cns.utexas.edu/news/the-tool-maker-the-double-life-of-everett-stone

New cancer treatment uses enzymes to boost immune system and fight back

/in /by

In our most recent paper, we show that administration of a pharmacologically optimized enzyme (PEGylated kynureninase; hereafter referred to as PEG-KYNase) that degrades Kyn into immunologically inert, nontoxic and readily cleared metabolites inhibits tumor growth. Enzyme treatment was associated with a marked increase in the tumor infiltration and proliferation of polyfunctional CD8+ lymphocytes. We show that PEG-KYNase administration had substantial therapeutic effects when combined with approved checkpoint inhibitors or with a cancer vaccine for the treatment of large B16-F10 melanoma, 4T1 breast carcinoma or CT26 colon carcinoma tumors. PEG-KYNase mediated prolonged depletion of Kyn in the TME and reversed the modulatory effects of IDO1/TDO upregulation in the TME.

https://www.nature.com/articles/nbt.4180

http://www.worldpharmanews.com/research/4522-new-cancer-treatment-uses-enzymes-to-boost-immune-system-and-fight-back

GRA George Delidakis wins Poster Prize at EMBO Antibodies Conference

/in /by

GRA George Delidakis recently attended the EMBO Antibody and Complement: Effector functions, Therapies and Technologies Conference in Girona, Spain and was awarded one of two Poster Awards.

The Conference was attended by an international group of faculty, students, and scientists from the biotech industry who work on understanding the diverse and complex roles of antibodies in the context of the broader immune system. In his poster, he presented our lab’s work on generating a panel of engineered antibodies which -unlike wildtype antibody- display unique specificity to each member of the Fc gamma receptor family, a family of receptors that are critical in linking the innate and adaptive branches of immunity. For his presentation, he was awarded one of the two Poster Awards, along with a cash prize.

The research was sponsored by the Clayton Foundation for Research.

Congratulations George!